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Optimization of bioactive compounds extraction assisted by microwave parameters from Kappaphycus alvarezii using RSM and ANFIS modeling

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Abstract

The main focus of present study is to optimize the extraction parameters for maximum yield of biomass, β-carotene and chlorophyll content and their antioxidants from Kappaphycus alvarezii by response surface methodology (RSM) along with adaptive neuro-fuzzy inference system (ANFIS). Central composite rotatable design of RSM was applied to optimize the effect of microwave-assisted extraction (MAE) parameters. In this way, four parameters, such as methanol concentration (X1: 65–80%), microwave power (X2: 15–20%), radiation induced temperature (X3: 45–60 °C) and radiation exposure time (X4: 7.5–15 min) at five levels were selected. Optimal conditions were found as X1 = 80%, X2 = 20%, X3 = 45 °C and X4 = 12.5–14.5 min, under this condition, % yield of biomass (y1) = 86.231–87.212%, β-carotene and chlorophyll content (y2) = 77.348–82.841 mg/gm, %DPPHsc (y3) = 69.362–72.768%, %ABTSsc (y4) = 66.282–70.621%, FRAP (y5) = 62.654–64.381 μg mol (Fe(II))/g has been obtained. Also, optimized values of MAE parameters were compared and verified with ANFIS. Further, the major bioactive compounds present in the optimally obtained extract of K. alvarezii was identified through FTIR, gas chromatography-mass spectrum and HPLC.

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Acknowledgements

The authors are grateful to the management of Kalasalingam Academy of Research and Education, Krishnankoil, India for research fellowships and utilizing research facilities. We also thank Prof. Z. Maciej Gliwicz, Ms. Ewa Babkiwicz, and Dr. Piotr Maszczyk, Department of Hydrobiology, Faculty of Biology, University of Warsaw, Warszawa, Poland, for timely help and support.

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Authors and Affiliations

  1. Department of Biotechnology, Kalasalingam Academy of Research and Education, Krishnankoil, 626126, India

    Suraj Baskararaj, Sankarganesh Arunachalam, Uma Priya Mohan & Selvaraj Kunjiappan

  2. Department of Research and Development, Saraswathi Institute of Medical Sciences, NH-24, Anwarpur, Pilkhuwa, Hapur, 245304, India

    Panneerselvam Theivendren

  3. School of Mechanical Engineering, Vellore Institute of Technology, Vellore, 632014, India

    Ponnusamy Palanisamy

  4. National Cyber Defence Research Centre, Kalasalingam Academy of Research and Education, Krishnankoil, 626126, India

    Suthendran Kannan

  5. Department of Pharmaceutical Chemistry, Faculty of Pharmacy, M S Ramaiah University of Applied Sciences, Gnanagangothri Campus, Bengaluru, 560054, India

    Parasuraman Pavadai

  6. Department of Pharmacy, Birla Institute of Technology and Science, Pilani, 333031, India

    Murugesan Sankaranarayanan

  7. School of Computer Science and Engineering, Vellore Institute of Technology, Vellore, 632014, India

    Lokeshkumar Ramasamy

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  1. Suraj Baskararaj
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  2. Panneerselvam Theivendren
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Correspondence to Selvaraj Kunjiappan.

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Baskararaj, S., Theivendren, P., Palanisamy, P. et al. Optimization of bioactive compounds extraction assisted by microwave parameters from Kappaphycus alvarezii using RSM and ANFIS modeling. Food Measure 13, 2773–2789 (2019). https://doi.org/10.1007/s11694-019-00198-1

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  • DOI: https://doi.org/10.1007/s11694-019-00198-1

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