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Shoot proliferation and callus regeneration from nodular buds of Drepanostachyum luodianense

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Abstract

This research reports on an efficient shoot proliferation and callus regeneration system for bamboo. Young, semi-lignified branches with one lateral bud from Drepanostachyum luodianense (Yi et R. S. Wang) Keng f. were used as explants. Disinfection with 0.1% HgCl2 for 8 min was the optimum treatment and the best medium for bud initiation was Murashige and Skoog (MS) medium containing 3.0 mg L−1 6-benzyladenine (BA). Multiple shoots were induced from nodal shoot segments on MS medium containing 5.0 mg L−1 BA, 0.5 mg L−1 kinetin (Kin), and 1.0 mg L−1 naphthaleneacetic acid (NAA). The highest frequency of callus formation (65.6%) was on MS medium containing 4.0 mg L−1 2,4-dichlorophenoxyacetic acid (2, 4-D), 0.5 mg L−1 NAA, and 0.1 mg L−1 thidiazuron (TDZ). The optimum medium for callus proliferation was MS medium with 4 mg L−1 2,4-D, 0.5 mg L−1 TDZ and 0.5 mg L−1 NAA, and the optimum hormone combination was 4 mg L−1 BA + 0.5 mg L−1 NAA for callus redifferentiation (up to 85.6%). A 100% rooting was achieved on MS medium supplemented with 2.0 mg L−1 NAA and 0.5 mg L−1 3-indole butyric acid (IBA). Rooted plantlets were acclimatized in a greenhouse in humus soil + perlite (1:1) substrate. These micropropagated callus induction and regeneration systems for bamboo will be useful for genetic engineering and multiplication.

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References

  • Agnihotri RK, Nandi SK (2009) In vitro shoot cut: a high frequency multiplication and rooting method in the bamboo Dendrocalamus hamiltonii. Biotechnolgy 8:259–263

    Google Scholar 

  • Arya S, Kant A, Sharma D, Arya ID (2008a) Micropropagation of two economically important bamboos: Drepanostachyum falcatum (Nees) Keng and Bambusa balcooa Roxb. Ind For 134:1211–1221

    Google Scholar 

  • Arya S, Satsangi R, Arya ID (2008b) Large scale plant production of edible bamboo Dendrocalamus asper through somatic embryogenesis. Bamboo Sci Cult J Am Bamboo Soc 21:21–31

    Google Scholar 

  • Banik RL (1995) A manual for vegetative propagation of bamboos. INBAR Technical Report No. 6. IDRC, Canada/FAO—FORTIP/BFRI, 66p

  • Chang WC (1995) Somatic embryogenesis of Bambusa oldhamii, Bambsa beecheyana and Sinocalamus latiflora. Somatic embryogenesis in woods plants. Series: Forestry Sciences, vol 44–46. Springer, Boston, pp 53–65. https://doi.org/10.1007/978-94-011-0491-3_4

    Book  Google Scholar 

  • Cheah KT, Chaille LC (2011) Somatic embryogenesis from mature Bambusa ventricosa. Biotechnolgy 11:1–5

    Google Scholar 

  • Das M, Pal A (2005) In vitro, regeneration of Bambusa balcooa Roxb: factors affecting changes of morphogenetic competence in the axillary buds. Plant Cell Tissue Org Cult 81:109–112

    Article  CAS  Google Scholar 

  • Eiman EE, Mohamed SE (2008) In Vitro Morphogenesis and Plant Regeneration of Bamboos (Oxytenanthera abyssinica A. Rich. Munro). Int J Sustain Crop Prod 3:72–79

    Google Scholar 

  • Gielis J, Peeters H, Gillis K, Oprins J, Debergh PC (2001) Tissue culture strategies for genetic improvement of bamboo. Acta Hort 552:195–203

    Article  Google Scholar 

  • Gillis K, Gielis J, Peeters H, Dhooghe E, Oprins J (2007) Somatic embryogenesis from mature Bambusa balcooa Roxburgh as basis for mass production of elite forestry bamboos. Plant Cell Tissue Org Cult 91:115–123

    Article  Google Scholar 

  • Hassan AAE, Debergh P (1987) Embryogenesis and plantlet development in the bamboo Phyllostachys viridis (Young) McClure. Plant Cell Tissue Org Cult 10:73–77

    Article  Google Scholar 

  • Huang LC, Huang BL (1995) Loss of species distinguishing trait among regenerated Bambusa ventricosa McClure plants. Plant Cell Tissue Org Cult 42:109–111

    Article  Google Scholar 

  • Huetteman CA, Preece JE (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tissue Org Cult 33:105–119

    Article  CAS  Google Scholar 

  • Islam N, Rahman M (2005) Micro-cloning in commercially important six bamboo species for mass propagation and at a large scale cultivation. Plant Tissue Cult Biotechnol 15:103–111

    Google Scholar 

  • Jiménez VM, Castillo J, Tavares E, Guevara E, Montiel M (2006) In vitro propagation of the neotropical giant bamboo, Guadua angustifolia kunth, through axillary shoot proliferation. Plant Cell Tissue Org Cult 86:389–395

    Article  Google Scholar 

  • Jullien F, Van KTT (1994) Micropropagation and embryoid formation from young leaves of Bambusa glaucescens ‘Golden Goddess’. Plant Sci 98:199–207

    Article  CAS  Google Scholar 

  • Komatsu YH, Batagin-Piotto KD, Brondani GE, Gonçalves AN, Almeida MD (2011) In vitro morphogenic response of leaf sheath of Phyllostachys bambusoides. J For Res 22:209–215

    Article  CAS  Google Scholar 

  • Liese W, Köhl M (2015) Bamboo: the plant and its uses. Springer, Heidelberg. https://doi.org/10.1007/2F978-3-319-14133-6

    Book  Google Scholar 

  • Lin CC, Lin CS, Chang WC (2003) In vitro flowering of Bambusa edulis and subsequent plantlet survival. Plant Cell Tissue Org Cult 72:71–78

    Article  CAS  Google Scholar 

  • Lin CS, Lin CC, Chang WC (2004) Effect of thidiazuron on vegetative tissue-derived somatic embryogenesis and flowering of bamboo Bambusa edulis. Plant Cell Tissue Org Cult 76:75–82

    Article  CAS  Google Scholar 

  • Lin CS, Liang C, Hsaio H, Lin M, Chang WC (2007) In vitro flowering of green and albino Dendrocalamus latiflorus. New For 34:177–186

    Article  Google Scholar 

  • Mehta R, Sharma V, Sood A, Sharma M, Sharma RK (2011) Induction of somatic embryogenesis and analysis of genetic fidelity of in vitro derived plantlets of Bambusa nutans Wall using AFLP markers. Eur J For Res 130:729–736

    Article  Google Scholar 

  • Mudoi KD, Borthakur M (2009) In vitro micropropagation of Bambusa balcooa Roxb. through nodal explants from field grown culms and scope for upscaling. Curr Sci 96:962–966

    Google Scholar 

  • Mudoi KD, Saikia SP, Goswami A, Bora D, Borthakur M (2009) Micropropagation of important bamboos: a review. Afr J Biotechnol 12:2770–2785

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Nielsen JM, Brandt K, Hansen J (1993) Long-term effects of thidiazuron are intermediate between benzyladenine, kinetin or isopentenyladenine in Miscanthus sinensis. Plant Cell Tissue Org Cult 35:173–179

    Article  CAS  Google Scholar 

  • Ogita S (2005) Callus and cell suspension culture of bamboo plant, Phyllostachys nigra. Plant Biotechnol 22:119–125

    Article  CAS  Google Scholar 

  • Ogita S, Kikuchi N, Nomura T, Kato Y (2011) A practical protocol for particle bombardment-mediated transformation of Phyllostachys bamboo suspension cells. Plant Biotechnol 28(1):43–50

    Article  Google Scholar 

  • Ramanayake SMSD, Maddegoda KMMN, Vitharana MC, Chaturani GDG (2008) Root induction in three species of bamboo with different rooting abilities. Sci Hortic 118:270–273

    Article  CAS  Google Scholar 

  • Rout GR, Das P (1994) Somatic embryogenesis and in vitro flowering of 3 species of bamboo. Plant Cell Rep 13(12):683–686

    Article  CAS  Google Scholar 

  • Rout GR, Das P (1997) In vitro plant regeneration via callogenesis and organogenesis in Bambusa vulgaris. Biol Plant 39:515–522

    Article  CAS  Google Scholar 

  • Saini H, Arya ID, Arya S, Sharma R (2016) In vitro micropropagation of himalayan weeping bamboo, Drepanostachyum falcatum. Am J Plant Sci 7:1317–1324

    Article  CAS  Google Scholar 

  • Saxena S (1990) In vitro propagation of the bamboo (Bambusa tulda Roxb.) through shoot proliferation. Plant Cell Rep 9:431–434

    Article  CAS  Google Scholar 

  • Saxena S, Bhojwani SS (1993) In vitro clonal multiplication of 4-year-old plants of the bamboo, Dendrocalamus longispathus Kurz. In Vitro Cell Dev Plant 29:135–142

    Article  Google Scholar 

  • Saxena S, Dhawan V (1999) Regeneration and large-scale propagation of bamboo (Dendrocalamus strictus Nees) through somatic embryogenesis. Plant Cell Rep 18:438–443

    Article  CAS  Google Scholar 

  • Singh M, Jaiswal U, Jaiswal VS (2000) Thidiazuron-induced in vitro flowering in Dendrocalamus strictus Nees. Curr Sci 79:1529–1530

    CAS  Google Scholar 

  • Sood A, Ahuja PS, Sharma M, Sharma OP, Godbole S (2002) In vitro protocols and field performance of elites of an important bamboo Dendrocalamus hamiltonii Nees et Arn. Ex Munro. Plant Cell Tissue Org Cult 71:55–63

    Article  CAS  Google Scholar 

  • Wei Q, Cao J, Qian W, Xu M, Li Z, Ding Y (2015) Establishment of an efficient micropropagation and callus regeneration system from the axillary buds of Bambusa ventricosa. Plant Cell Tissue Org Cult 122:1–8

    Article  Google Scholar 

  • Woods SH, Phillips GC, Woods JE, Collins GB (1992) Somatic embryogenesis and plant regeneration from zygotic embryo explants in mexican weeping bamboo, Otatea acuminata aztecorum. Plant Cell Rep 11:257–261

    CAS  PubMed  Google Scholar 

  • Yasodha R, Kamala S, Kumar SPA, Kumar PD, Kalaiarasi K (2008) Effect of glucose on in vitro rooting of mature plants of Bambusa nutans. Sci Hortic 116:113–116

    Article  CAS  Google Scholar 

  • Yeh ML, Chang WC (1986) Plant regeneration through somatic embryogenesis in callus culture of green bamboo (Bambusa oldhamii Munro). Theor Appl Genet 73:161–163

    Article  CAS  Google Scholar 

  • Zhang N, Fang W, Shi Y, Liu Q, Yang H, Gui R, Lin X (2010) Somatic embryogenesis and organogenesis in Dendrocalamus hamiltonii. Plant Cell Tissue Org Cult 103:325–332

    Article  CAS  Google Scholar 

Download references

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Author notes

  1. Shuyan Lin, Guohua Liu and Tingting Guo have contributed equally to this work.

Authors and Affiliations

  1. Co-Innovation Center for Sustainable Forestry in Southern China, Bamboo Research Institute, College of Biology and the Environment, Nanjing Forestry University, Nanjing, 210037, Jiangsu, People’s Republic of China

    Shuyan Lin, Guohua Liu, Tingting Guo, Li Zhang & Yulong Ding

  2. College of Life Science, Southwest Forestry University, Kunming, 650224, People’s Republic of China

    Shuguang Wang

Authors
  1. Shuyan Lin
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  2. Guohua Liu
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  3. Tingting Guo
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  4. Li Zhang
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  5. Shuguang Wang
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  6. Yulong Ding
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Corresponding authors

Correspondence to Shuguang Wang or Yulong Ding.

Additional information

Project funding: This work was supported by the National Key Research and Development Program of China under Grant 2016YFD0600901; Jiangsu Province “SANXIN” Support Project under Grant LYSX [2016]04; National Natural Science Foundation for Young Scholars of China under Grant 31000294; and Priority Academic Program Development of Jiangsu Higher Education Institutions.

The online version is available at http://www.springerlink.com

Corresponding editor: Zhu Hong.

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Lin, S., Liu, G., Guo, T. et al. Shoot proliferation and callus regeneration from nodular buds of Drepanostachyum luodianense. J. For. Res. 30, 1997–2005 (2019). https://doi.org/10.1007/s11676-018-0772-9

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  • DOI: https://doi.org/10.1007/s11676-018-0772-9

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