Skip to main content
Log in

An improvised in vitro vegetative propagation technique for Bambusa tulda: influence of season, sterilization and hormones

  • Original Paper
  • Published:
Journal of Forestry Research Aims and scope Submit manuscript

Abstract

Season and concentration of sterilizing agents play a significant role for establishment of aseptic in vitro shoot cultures and sprouting of nodal explants from field growing culms of bamboo species. In the present investigation the nodal segment explants of Bambusa tulda Roxb collected in different seasons and treated with various concentrations of HgCl2 showed significant variation in aseptic culture establishment and bud break. The rainy season (July–August) recorded with highest of 78% aseptic culture establishment whereas autumn recorded with lowest 46%. Summer and winter seasons emerged to be the best period, registering > 60% in vitro bud break. On the other hand, the autumn season had the lowest value for bud break, i.e. 42%. Among different doses of sterilizing agent tried, HgCl2 0.1% found to be suitable for maximum aseptic culture establishment (66%) as well as bud break (59%). However, among the interactions, summer season and the dose of 0.1% HgCl2 exhibited maximum of 73% response for both aseptic culture establishment and bud break. MS medium (liquid) enriched with 5.0 µM BA + 5.0 µM Kn [Kinetin (N6-Furfuryladenine)] with additional supplementation of 100 µM glutamine + 0.1 µM IAA supported a maximum in vitro shoot multiplication of 4.75 fold. The proliferated shoots were successfully rooted on MS medium (liquid) supplemented 40 µM coumarin. The plantlets transferred to the polythene bags showed 98% survival.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

Abbreviations

BA:

6-Benzyladenine

HgCl2 :

Mercuric Chloride

Kn:

Kinetin (N6-Furfuryladenine)

IAA:

Indole-3 acetic acid

MS:

Murashige and Skoog (1962)

References

  • Agnihotri K, Ansari SA (2000) Adventitious rhizogenesis in relation to seasonal variation, size of culm branch cuttings and IAA treatment in bamboos. Indian For 126:971–984

    Google Scholar 

  • Arshad SM, Kumar A, Bhatnagar SK (2005) Micropropagation of Bambusa wamin through shoot proliferation of mature nodal explants. J Biol Res 3:59–66

    CAS  Google Scholar 

  • Arya ID, Arya S (1997) In vitro culture and establishment of exotic bamboo Dendrocalamus asper. Indian J Exp Biol 35:1252–1255

    Google Scholar 

  • Arya ID, Satsangi R, Arya S (2001) Rapid micropropagation of edible bamboo Dendrocalamus asper. J Sustain For 114:103–114

    Article  Google Scholar 

  • Bag N, Chandra S, Palni LMS, Nandi SK (2000) Micropropagation of Dev-ringal. Plant Sci 156:125–135

    Article  PubMed  CAS  Google Scholar 

  • Bisht P, Pant M, Kant A (2010) In vitro propagation of Gigantochloa atroviolaceae Widjaja through nodal explants. J Am Sci 10:1019–1025

    Google Scholar 

  • Chakraborty D (1988) Utilization of bamboos as raw material in the handicraft industries in Tripura. Indian For 114:635–636

    Google Scholar 

  • Das M, Pal A (2005) In vitro regeneration of Bambusa balcooa Roxb.: factors affecting changes of morphogenetic competence in the axillary buds. Plant Cell, Tissue Organ Cult 81:109–112

    Article  CAS  Google Scholar 

  • Gomez KA, Gomez AA (1984) Stastical procedures for agricultural research, 2nd edn. Wiley, Singapore, pp 346–347

    Google Scholar 

  • Kumar R, Sharma K, Agrawal V (2005) In vitro clonal propagation of Holarrhena antidysenterica (l.) wall. through nodal explants from mature trees. In Vitro Cell Dev Biol Plant 41:137–144

    Article  CAS  Google Scholar 

  • Mishra Y, Rana PK, Shirin F, Ansari SA (2001) Augmenting in vitro shoot multiplication by vipul and rhizogenesis by rice bran extract in Dendrocalamus strictus. Indian J Exp Biol 39:165–169

    PubMed  CAS  Google Scholar 

  • Mishra Y, Patel P, Yadav S, Shirin F, Ansari SA (2008) A micropropagation system for cloning of Bambusa tulda Roxb. Sci Hortic 115:315–318

    Article  CAS  Google Scholar 

  • Mohan Ram HY, Harigopal B (1981) Some observations on the flowering of bamboos in Mizoram. Curr Sci 50:708–710

    Google Scholar 

  • Moutia M, Dookun A (1999) Evaluation of surface sterilization and hot water treatments on bacterial contaminations in bud culture of sugarcane. Exp Agric 35:265–274

    Article  Google Scholar 

  • Mudoi KD, Borthakur M (2009) In vitro micropropagation of Bambusa balcooa Roxb. through nodal explants from field-grown culms and scope for upscaling. Curr Sci 7:962–966

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Negi D, Saxena S (2011) Micropropagation of Bambusa balcooa Roxb. through axillary shoot proliferation. In Vitro Cell Dev Biol Plant 47:604–610

    Article  CAS  Google Scholar 

  • Ogita S (2005) Callus and cell suspension culture of bamboo plant Phyllostachys nigra. Plant Biotechnol 22:119–125

    Article  CAS  Google Scholar 

  • Pollock BM, Goodwin RH, Greene S (1954) Studies on roots. II. Effects of coumarin, scopoletin and other substances on growth. Am J Bot 4:521–529

    Article  Google Scholar 

  • Ramanayake SMSD, Yakandawala K (1997) Micropropagation of the giant bamboo (Dendrocalamus giganteus Munro) from nodal explants of field grown culms. Plant Sci 129:213–223

    Article  CAS  Google Scholar 

  • Sanjaya, Rathore TS, Ravishankar Rai V (2005) Micropropagation of Pseudoxytenanthera stocksii Munro. In Vitro Cell Dev Biol Plant 41(3):333–337

    Article  CAS  Google Scholar 

  • Saxena S (1990) In vitro propagation of the bamboo (Bambusa tulda Roxb.) through shoot proliferation. Plant Cell Rep 9:431–434

    Article  PubMed  CAS  Google Scholar 

  • Saxena S, Bhojwani SS (1991) Towards regeneration and mass propagation of bamboos through tissue culture, In: Bamboo in Asia and the Pacific, proceedings 4th international bamboo workshop, International Development Research Centre FAO-UNDP, Chingmai, Tha land, pp 157–164

  • Saxena S, Bhojwani SS (1993) In vitro clonal multiplication of 4 year old plants of bamboo, Dendrocalamus longispathus Kurz. In vitro Cell Dev Biol Plant 29:135–142

    Article  Google Scholar 

  • Sharma P, Sarma KP (2013) In vitro propagation of Bambusa tulda: an important plant for better environment. J Environ Res Dev 7:1216–1223

    Google Scholar 

  • Srivastava RC (1990) Bamboo new raw material for phytosterols. Curr Sci 59:1311–1334

    Google Scholar 

  • Tartoura K, Andrea DR, Youssef S (2004) Synergistic interaction between coumarin 1,2-benzopyrone and indole-3-butyric acid in stimulating adventitious root formation in Vigna radiata (L.) Wilczek cuttings: I. Endogenous free and conjugated IAA and basic isoperoxidases. Plant Growth Regul 42:253–262

    Article  CAS  Google Scholar 

  • Tewari DN (1992) A monograph on bamboo, International Book Distributors. Valley Offset Printers and Publishers, Dehra Dun, p 498

    Google Scholar 

  • Uppin SF (1980) High yielding bamboos for artificial regeneration. Myforest 16:55–62

    Google Scholar 

  • Upreti TC, Sundriyal RC (2001) Bamboo and cane resources of Arunachal Pradesh: utilization pattern and implications for management. Bamboo Sci Cult 15:20–34

    Google Scholar 

  • Woods SH, Woods JE, Phillips GC, Collins GB (1991) Somatic embryogenesis and plant regeneration from Mexican Weeping Bamboo Otatea acuminata aztecorum. In: Bamboo in Asia and the Pacific, proceedings 4th international bamboo workshop. Thailand: International Development Research Centre FAO-UNDP, Chingmai, pp 153–156

  • Yadav S, Patel P, Shirin F, Mishra Y, Ansari SA (2008) In vitro clonal propagation of 10-year-old clumps of Bambusa nutans. J Bamboo Rattan 7(3&4):201–210

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Yogeshwar Mishra.

Additional information

Project funding: This study was supported by Project of Commercial production of quality planting material of bamboo species (No. 213/2014/Gen-1 (NBM) (3).

The online version is available at http://www.springerlink.com

Corresponding editor: Zhu Hong.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Bhadrawale, D., Mishra, J.P. & Mishra, Y. An improvised in vitro vegetative propagation technique for Bambusa tulda: influence of season, sterilization and hormones. J. For. Res. 29, 1069–1074 (2018). https://doi.org/10.1007/s11676-017-0569-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11676-017-0569-2

Keywords

Navigation