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Qufeng Tongluo Prescription (祛风通络方) inhibits mesangial cell proliferation and promotes apoptosis through regulating cell cycle progression

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Abstract

Objective

To study the effects and possible underlying mechanism of Qufeng Tongluo Prescription (祛风通络方, QFTL) on the regulation of mesangial cells (MCs) proliferation and apoptosis.

Methods

The MCs used in this experiment have undergone five passages induced by lipopolysaccharide (LPS). Changes in the proliferation, apoptosis, cell cycle regulatory proteins and mRNA expression levels of the MCs after administration of Benazepril or QFTL were measured by methyl thiazolyl tetrazolium (MTT) reduction assay, flow cytometry, Western blot and quantitative real-time polymerase chain reaction (qRT-PCR), respectively.

Results

The addition of Benazepril or QFTL serum inhibited LPS-induced MC proliferation after treatment for 24, 48 and 72 h, respectively (P<0.05 or P<0.01). Moreover, the inhibitory effect is more significant in the QFTL group at 48 h (P<0.05). Compared with the control group, LPS-induced cell proliferation decreased the number of cells in G1 phase versus cells in S and G2/M phases, while the addition of QFTL and Benazepril serum increased the ratio of cells at G1 phase (P<0.05 or P<0.01) to cells at S phase (P<0.01), implicating the cell cycle inhibition effect exerted by QFTL. LPS decreased the level of MC apoptosis, compared with the control group (P<0.05), while QFTL and Benazepril serum increased the level of MC apoptosis (P<0.01). Moreover, the difference between the QFTL group and the Benazepril group was statistically significant (P<0.01). Compared with the control group, the protein and mRNA expression levels of cylinD1, cyclin dependent kinase 2 (CDK2) and p21 were significantly increased (P<0.05 or P<0.01), p27 was decreased but with no statistical significance (P>0.05); After being treated with QFTL and Benazepril serum, the protein and mRNA expression levels of cylinD1, CDK2, p21 were decreased and p27 increased significantly (P<0.05 or P<0.01); Compared with the Benazepril group, QFTL show better effects on protein and mRNA expression levels of cylinD1, CDK2 (P<0.05 or P<0.01) and p21 protein expression (P<0.05).

Conclusion

QFTL inhibits MCs proliferation, promotes MCs apoptosis through an underlying mechanism of down-regulating the protein and mRNA expression levels of cylinD1, CDK2, p21 and up-regulation of the expression level of p27.

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Authors and Affiliations

  1. Department of Integrated Traditional Chinese and Western Medicine, Second Affiliated Hospital of Medical School of Xi’an Jiaotong University, Xi’an, 710004, China

    Xi-li Wu  (吴喜利), Peng An  (安 鹏), Bing-yu Ye  (叶冰玉), Wan-sen Sun  (孙万森), Zhu Wang  (王 竹) & Cheng-lin Qiao  (乔成林)

  2. School of Public Health of Xi’an Jiaotong University, Xi’an, 710061, China

    Xing-min Shi  (石兴民)

  3. Department of Nephrology, Second Affiliated Hospital of Medical School of Xi’an Jiaotong University, Xi’an, 710004, China

    Rong-guo Fu  (付荣国)

Authors
  1. Xi-li Wu  (吴喜利)
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  2. Peng An  (安 鹏)
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  3. Bing-yu Ye  (叶冰玉)
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  4. Xing-min Shi  (石兴民)
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  5. Wan-sen Sun  (孙万森)
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  6. Rong-guo Fu  (付荣国)
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  7. Zhu Wang  (王 竹)
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  8. Cheng-lin Qiao  (乔成林)
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Corresponding author

Correspondence to Xi-li Wu  (吴喜利).

Additional information

Supported by the National Natural Science Foundation of China (No. 30801504, No. 30901926, No. 81100530 and No. 81070590)

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Wu, Xl., An, P., Ye, By. et al. Qufeng Tongluo Prescription (祛风通络方) inhibits mesangial cell proliferation and promotes apoptosis through regulating cell cycle progression. Chin. J. Integr. Med. 19, 927–934 (2013). https://doi.org/10.1007/s11655-013-1655-8

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  • DOI: https://doi.org/10.1007/s11655-013-1655-8

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