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A filter paper-based liquid culture system for citrus shoot organogenesis—a mixture-amount plant growth regulator experiment

  • Plant Tissue Culture
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Abstract

This study determined the effects of a static liquid culture system on shoot regeneration from citrus epicotyl explants. A mixture-amount experiment was used to determine the effects of zeatin riboside (ZR), 6-benzylaminopurine (BA), and indole-3-acetic acid (IAA) on two citrus types—citrange (Citrus sinensis ‘Washington’ L. Osbeck. × Poncirus trifoliata L. Raf var. Carrizo) and sweet orange (Citrus sinensis L. Osbeck var. ‘Ridge Pineapple’). A liquid culture system comprising a Petri dish, cellulose filter paper, and liquid culture medium was used. Shoot regeneration experiments were conducted over 6 wk that included 2 wk in the dark followed by 4 wk in the light. Three responses were measured: (1) number of explants forming buds and/or shoots, (2) number of explants with shoots > 2 mm, and (3) overall explant and shoot quality. The effects of paper disc number, liquid medium volume, and explant size on shoot regeneration were determined. High-quality shoots were produced from explants cultured in 5.25 to 12 mL medium volume and explant sizes ranging from 2 to 15 mm. The effects of the plant growth regulators were similar for the two citrus types and were as follows: (1) use of ZR or BA resulted in high-quality shoot production; (2) ZR and BA were not synergistic; (3) culture in 20 μM ZR resulted in the highest shoot production; (4) BA and IAA were strongly synergistic, with the greatest production with BA when IAA was included in the mixture; and (5) ZR and IAA were antagonistic, particularly with Ridge Pineapple.

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Acknowledgments

We thank Eldridge Wynn for his careful preparation of the media formulations, assistance with the growth of the plant cultures, and collection of the data.

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  1. United States Department of Agriculture, Agricultural Research Service, U.S. Horticultural Research Laboratory, Fort Pierce, FL, 34945, USA

    Randall P. Niedz & Mizuri Marutani-Hert

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  1. Randall P. Niedz
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  2. Mizuri Marutani-Hert
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Correspondence to Randall P. Niedz.

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Editor: Pamela Weathers

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Niedz, R.P., Marutani-Hert, M. A filter paper-based liquid culture system for citrus shoot organogenesis—a mixture-amount plant growth regulator experiment. In Vitro Cell.Dev.Biol.-Plant 54, 658–671 (2018). https://doi.org/10.1007/s11627-018-9940-z

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