Skip to main content
SpringerLink
Log in

Induction medium osmolality improves microspore embryogenesis in wheat and triticale

  • Plant Tissue Culture
  • Published:
In Vitro Cellular & Developmental Biology - Plant Aims and scope Submit manuscript

Abstract

The objective of this study was to determine the effect of induction medium osmolality on embryogenesis and green plant production in wheat and triticale. Isolated microspores of wheat and triticale were subjected to a range of osmolality (300–500 mOsm kg−1) using mannitol. In both species, the maximum number of embryo-like structures (ELS) and green plants were obtained at 350 mOsm kg−1 when the induction medium was supplemented with 9.1 g L−1 of mannitol. A sharp decline in microspore response was observed at higher osmolality. These results demonstrate the effect of osmolality on induction of ELS and production of green plants indicating that the process of microspore embryogenesis can be improved in wheat and triticale by increasing osmolality of the induction medium to 350 mOsm kg−1.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1.
Figure 2.
Figure 3.
Figure 4.

Similar content being viewed by others

References

  • Attree SM, Fowke LC (1993) Embryogeny of gymnosperms—advances in synthetic seed technology of conifers. Plant Cell Tissue Organ Cult 35:1–35

    Article  CAS  Google Scholar 

  • Ayed OS, Buyser JD, Picard E, Trifa Y, Amara HS (2010) Effect of pretreatment on isolated microspores culture ability in durum wheat (Triticum turgidum subsp. durum Desf.). J Plant Breed Crop Sci 2:30–38

    Google Scholar 

  • Cistue L, Romagosa I, Batlle F, Echavarri B (2009) Improvements in the production of doubled haploids in durum wheat (Triticum turgidum L.) through isolated microspore culture. Plant Cell Rep 28:727–735

    Article  CAS  PubMed  Google Scholar 

  • Cistue L, Soriano M, Castillo AM, Valles MP, Sanz JM, Echavarri B (2006) Production of doubled haploids in durum wheat (Triticum turgidum L.) through isolated microspore culture. Plant Cell Rep 25:257–264

    Article  CAS  PubMed  Google Scholar 

  • Cram WJ (1984) Mannitol transport and suitability as an osmoticum in root-cells. Physiol Plant 61:396–404

    Article  CAS  Google Scholar 

  • Custers JBM (2003) Microspore culture in rapeseed (Brassica napus L.). In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Doubled haploid production in crop plants: a manual, 1st edn. Kluwer, Dordrecht Netherlands, pp 185–194

    Chapter  Google Scholar 

  • Delaitre C, Ochatt S, Deleury E (2001) Electroporation modulates the embryogenic responses of asparagus (Asparagus officinalis L.) microspores. Protoplasma 216:39–46

    Article  CAS  PubMed  Google Scholar 

  • Diamond JM, Wright EM (1969) Biological membranes—physical basis of ion and nonelectrolyte selectivity. Annu Rev Physiol 31:581–646

    Article  CAS  PubMed  Google Scholar 

  • Eudes F, Acharya S, Laroche A, Selinger LB, Cheng KJ (2003) A novel method to induce direct somatic embryogenesis, secondary embryogenesis and regeneration of fertile green cereal plants. Plant Cell Tissue Organ Cult 73:147–157

    Article  CAS  Google Scholar 

  • Ferrie AMR (2003) Microspore culture of Brassica species. In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Doubled haploid production in crop plants: a manual, 1st edn. Kluwer, Dordrecht Netherlands, pp 205–215

    Chapter  Google Scholar 

  • Garrido D, Chibi F, Matilla A (1995) Polyamines in the induction of Nicotiana tabacum pollen embryogenesis by starvation. J Plant Physiol 145:731–735

    Article  CAS  Google Scholar 

  • Grewal RK, Lulsdorf M, Croser J, Ochatt S, Vandenberg A, Warkentin TD (2009) Doubled-haploid production in chickpea (Cicer arietinum L.): role of stress treatments. Plant Cell Rep 28:1289–1299

    Article  CAS  PubMed  Google Scholar 

  • Guo YD, Pulli S (2000) Isolated microspore culture and plant regeneration in rye (Secale cereale L.). Plant Cell Rep 19:875–880

    Article  CAS  Google Scholar 

  • Hoekstra S, Vanzijderveld MH, Heidekamp F, Vandermark F (1993) Microspore culture of Hordeum vulgare L.—the influence of density and osmolality. Plant Cell Rep 12:661–665

    Article  CAS  PubMed  Google Scholar 

  • Hu TC, Kasha KJ (1999) A cytological study of pretreatments used to improve isolated microspore cultures of wheat (Triticum aestivum L.) cv. Chris. Genome 42:432–441

    Article  Google Scholar 

  • Immonen S, Robinson J (2000) Stress treatments and ficoll for improving green plant regeneration in triticale anther culture. Plant Sci 150:77–84

    Article  CAS  Google Scholar 

  • Kaur P, Bhalla JK (1998) Regeneration of haploid plants from microspore culture of pigeon pea (Cajanus cajan L.). Indian J Exp Biol 36:736–738

    Google Scholar 

  • Konzak CF, Polle EA, Liu W, Zheng Y (1999) Methods for generating doubled-haploid plants. US Patent No. US6362393

  • Kumlehn J, Serazetdinova L, Hensel G, Becker D, Loerz H (2006) Genetic transformation of barley (Hordeum vulgare L.) via infection of androgenetic pollen cultures with Agrobacterium tumefaciens. Plant Biotechnol J 4:251–261

    Article  CAS  PubMed  Google Scholar 

  • Levene H (1960) Robust tests for equality of variances. In: Olkin I, Hotelling H (eds) Contributions to probability and statistics. Stanford University Press, Palo Alto, CA, pp 278–292

    Google Scholar 

  • Lichter R (1981) Anther culture of Brassica napus in a liquid culture-medium. Zeitschrift Fur Pflanzenphysiologie 103:229–237

    Article  CAS  Google Scholar 

  • Lionneton E, Beuret W, Delaitre C, Ochatt S, Rancillac M (2001) Improved microspore culture and doubled-haploid plant regeneration in the brown condiment mustard (Brassica juncea). Plant Cell Rep 20:126–130

    Article  CAS  Google Scholar 

  • Massey FJ (1951) The Kolmogorov–Smirnov test for goodness of fit. J Am Stat Assoc 46:68–78

    Article  Google Scholar 

  • Mix G, Wang HM (1988) Invitro production of haploid cowpea plants (Vigna unguiculata L). Landbauforsch Volk 38:305–309

    Google Scholar 

  • Ochatt S, Pech C, Grewal R, Conreux C, Lulsdorf M, Jacas L (2009) Abiotic stress enhances androgenesis from isolated microspores of some legume species (Fabaceae). J Plant Physiol 166:1314–1328

    Article  CAS  PubMed  Google Scholar 

  • Roberts OSL, Dunwell JM (1990) Barley anther culture: pretreatment on mannitol stimulates production of microspore-derived embryos. Plant Cell Tiss Organ Cult 20:235–240

    Google Scholar 

  • SAS (2003) Release 9.2. SAS Institute Inc, Cary, NC, USA

    Google Scholar 

  • Steel RGD, Torrie JH, Dickey DA (1997) Principles and procedures of statistics: a biometrical approach. McGraw Hill, New York, USA

    Google Scholar 

  • Trip P, Krotkov G, Nelson CD (1964) Metabolism of mannitol in higher plants. Am J Bot 51:828–835

    Article  CAS  Google Scholar 

  • Wojnarowiez G, Caredda S, Devaux P, Sangwan R, Clement C (2004) Barley anther culture: assessment of carbohydrate effects on embryo yield, green plant production and differential plastid development in relation with albinism. J Plant Physiol 161:747–755

    Article  CAS  PubMed  Google Scholar 

  • Xie JH, Gao MW, Cai QH, Liang ZQ, Xue QZ (1996) Effects of donor plant growth status and preculture temperature on isolated microspore culture ability in japonica rice (Oryza sativa L). Cereal Res Commun 24:133–138

    Google Scholar 

  • Xu N, Coulter KM, Bewley JD (1990) Abscisic-acid and osmoticum prevent germination of developing alfalfa embryos, but only osmoticum maintains the synthesis of developmental proteins. Planta 182:382–390

    Article  CAS  PubMed  Google Scholar 

  • Zhao GL, Liu YZ, Yin AP, Li JB (1998) Germination of embryo in soybean anther culture. Chin Sci Bull 43:1991–1995

    Article  CAS  Google Scholar 

  • Zheng MY, Weng Y, Sahibzada R, Konzak CF (2003) Isolated microspore culture in maize (Zea mays L.) production of doubled-haploid via induced androgenesis. In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Doubled haploid production in crop plants: a manual, 1st edn. Kluwer, Dordrecht, pp 95–102

    Chapter  Google Scholar 

  • Zhou H, Zheng Y, Konzak CF (1991) Osmotic potential of media affecting green plant percentage in wheat anther culture. Plant Cell Rep 10:63–66

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

The research reported in this paper was supported by Western Grains Research Foundation, Canadian Wheat Board and Alberta Crop Industry Development Fund.

Author information

Authors and Affiliations

  1. Agriculture and Agri-Food Canada, 5403-1st Avenue South, Lethbridge, AB T1J 4B1, Canada

    Muhammad Asif, François Eudes, Harpinder Randhawa & Eric Amundsen

  2. Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5, Canada

    Muhammad Asif & Dean Spaner

Authors
  1. Muhammad Asif
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. François Eudes
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Harpinder Randhawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Eric Amundsen
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Dean Spaner
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to François Eudes.

Additional information

Editor: J. Forster

Rights and permissions

Reprints and permissions

About this article

Cite this article

Asif, M., Eudes, F., Randhawa, H. et al. Induction medium osmolality improves microspore embryogenesis in wheat and triticale. In Vitro Cell.Dev.Biol.-Plant 50, 121–126 (2014). https://doi.org/10.1007/s11627-013-9545-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11627-013-9545-5

Keywords

Search

Navigation