Abstract
An efficient system was established for a higher frequency of protocorm-like body (PLB) formation from the callus of Dendrobium candidum Wall ex Lindl. The calluses were induced from longitudinally bisected segments of protocorms and subcultured two times every 40d on Murashige and Skoog medium with macronutrients at half strength, micronutrients at full strength, 2% sucrose, and with 8.8μM 6-Benzylaminopurine. PLB formation was achieved when calluses were transferred onto the same basal medium without any plant growth regulators. PLBs developed into intact plantlets about 2cm in height and with four roots when on basal medium with 2.7μM 1-naphthaleneacetic acid. Plantlets were transplanted into vermiculite with a 95% survival rate in a greenhouse. Histological observations proved that globular somatic embryos could be produced from the inside and surface of the embryogenic callus during PLB formation.
Similar content being viewed by others
References
Arditti, J.; Ernst, R. Micropropagation of orchids. New York: Wiley; 1993.
Bagde, P.; Sharon, M. In vitro regeneration of Oncidium ‘Gower Ramsey’ by high frequency protocorm like bodies proliferation. J Plant Physiol 2: 10–14; 1997.
Begum, A.A.; Tamaki, M.; Tahara, M.; Kato, S. Somatic embryogenesis in Cymbidium through in vitro culture of inner tissue of protocorm-like bodies. J Jpn Soc Hortic Sci 63: 419–427; 1994.
Chang, C.; Chang, W.C. Plant regeneration from callus culture of Cymbidium ensifolium var. misericors. Plant Cell Rep 17: 251–255; 1998.
Chen, J.T.; Chang, C.; Chang, W.C. Direct somatic embryogenesis on leaf explants of Oncidium ‘Gower Ramsey’ and subsequent plant regeneration. Plant Cell Rep 19: 143–149; 1999.
Chen, J.T.; Chang, W.C. Efficient plant regeneration through somatic embryogenesis from callus of Oncidium (Orchidaceae). Plant Sci 160: 87–93; 2000.
Chen, J.T.; Chang, W.C. Effects of tissue culture conditions and explant characteristics on direct somatic embryogenesis in Oncidium ‘Gower Ramsey’. Plant Cell Tissue Organ Cult 69: 41–44; 2002.
Chen, T.Y.; Chang, W.C. Plant regeneration through direct shoot bud formation from leaf cultures of Paphiopedilum orchids. Plant Cell Tissue Organ Cult 76: 11–15; 2004.
Chen, W.; Cun, S.X. In vitro rapid propagation of stems of Dendrobium candidum Plant Physiol Commun 38: 145; 2002 (In Chinese).
Chengalrayan, K.; Hazra. S.; Gallo-Meagher, M. Histological analysis of somatic embryogenesis and organogenesis induced from mature zygotic embryo-derived leaflets of peanut (Arachis hypogaea. L.). Plant Sci 161: 415–421; 2001.
Chung, H.H.; Chen, J.T.; Chang, W.C. Cytokinins induce direct somatic embryogenesis of Dendrobium Chiendmai Pink and subsequent plant regeneration. In Vitro Cell Dev Biol Plant 41: 765–769; 2005.
Chung, J.D.; Chun, C.K.; Choi, S.O. Asymbiotic germination of Cymbidium ensifolium. J Korean Soc Hort Sci 26: 182–196; 1985.
Colli, S.; Kerbauy, G.B. Direct root tip conversion of Catasetun into protocorm-like bodies: effect of auxin and cytokinin. Plant Cell Tissue Organ Cult 33: 39–44; 1993.
Eady, C.C.; Butler, R.C.; Suo, Y. Somatic embryogenesis and plant regeneration from immature embryo cultures of onion (Allium cepa L.). Plant Cell Rep 18: 111–116; 1998.
He, J.B.; Zheng, C.Z.; Wang, S.L. Multiplication of protocorm of Dendrobium candidum. Acta Botanica Yunnanica 4: 211–212; 1982 (In Chinese).
Hou, P.Y.; Guo, S.X. Studies on transplanting suspension-cultured protocorms of Dendrobium candidum onto solid culture medium. China J Chinese Materia Medica 30: 729–732; 2005.
Huan, L.V.T.; Takamura. T.; Tanaka, M. Callus formation and plant regeneration from callus through somatic embryo structures in Cymbidium orchid. Plant Sci 166: 1443–1449; 2004.
Huang, L.T.; Baiocco, M.; Huy, B.P.; Mezzetti, B.; Santilocchi, R.; Rosati, P. Somatic embryogenesis in Canary Island date palm. Plant Cell Tissue Organ Cult 56: 1–7; 1999.
Ishii, Y.; Takamura, T.; Goi, M.; Tanaka, M. Callus induction and somatic embryogenesis of Phalaenopsis. Plant Cell Rep 17: 446–450; 1998.
Kerbauy, G.B. Plant regeneration of Oncidium varicosum (Orchidaceae) by means of root tip culture. Plant Cell Rep 3: 27–29; 1984.
Lee, Y.I.; Lee, N. Plant regeneration from protocorm-derived callus of Cypripedium formosanum. In Vitro Cell Dev Biol Plant 39: 475–479; 2003.
Li, S.; Shen, Z.H.; Qin, Z.; Wang, Y.F. Uptake rate of tracer elements by Lycium barbarum L. in somatic embryogenesis. J Radioanal Nucl Chem 250: 593–597; 2001.
Lim-Ho, C.L.; Lee, G.C. Clonal propagation of Oncidium from dormant buds on flower stalk. Malay Orchid Rev 22: 48–52; 1987.
Lu, M.C. High frequency plant regeneration from callus culture of Pleione formosana Hayata. Plant Cell Tissue Organ Cult 78: 93–96; 2004.
Luo, J.P.; Jia, J.F.; Gu, Y.H.; Liu, J. High frequency somatic embryogenesis and plant regeneration in callus cultures of Astragalus adsurgens Pall. Plant Sci 143: 93–99; 1999.
Malabadi, R.B.; Mulgund, G.S.; Kallappa, N. Micropropagation of Dendrobium nobile from shoot tip sections. J Plant Physiol 162: 473–478; 2005.
Malabadi, R.B.; Mulgund, G.S.; Nataraja, K. Efficient regeneration of Vanda coerulea, an endangered orchid using thidiazuron. Plant Cell Tissue Organ Cult 76: 289–293; 2004.
Morel, G.M. Proding virus-free Cymbidium. Am Orchid Soc Bull 29: 495–497; 1960.
Nayak, N.R.; Sahoo, S.; Patnaik, S.; Rath, S.P. Establishment of thin cross section (TCS) culture method for rapid micropropagation of Cymbidium aloifolium (L.) Sw. and Dendrobium nobile Lindl. (Orchidaceae). Sci Hortic 94: 107–116; 2002.
Nikam, T.D.; Bansude. G.M.; Aneesh Kumar, K.C. Somatic embryogenesis in sisal (Agave sisalana Perr. ex. Engelm). Plant Cell Rep 22: 188–194; 2003.
Roy, J.; Banerjee, N. Induction of callus and plant regeneration from shoot-tip explants of Dendrobium firmbriatum Lindl. var. oculatum Hk.f. Sci Hortic 97: 333–340; 2003.
Sagawa, Y.; Kunisaki, J. T. Clonal propagation of orchids by tissue culture. Proceedings of 5th congress, Plant tissue and cell culture, pp 683–684; 1982.
Sheelavanthmath, S.S., Murthy, H.N., Hema, B.P., Hahn, E.J., Paek, K.Y. High frequency of protocorm like bodies (PLBs) induction and plant regeneration from protocorm and leaf sections of Aerides crispum. Sci Hortic 106: 395–401; 2005.
Sheelavantmath, S.S.; Murthy, H.N.; Pyati, A.N.; Kumar, H.G..A.; Ravishankar, B.V. In vitro propagation of the endangered orchid, Geodorum densiflorum (Lam.) Schltr. through rhizome section culture. Plant Cell Tissue Organ Cult 60: 151–154; 2000.
Shiau, Y.J.; Nalawade, S.M.; Hsia, C.N.; Mulabacal, V.; Tsay, H.S. In vitro propagation of the Chinese medicinal plant, Dendrobium candidum Wall. Ex Lindl., from axenic nodal segments. In Vitro Cell Dev Biol Plant 41: 666–670; 2005.
Shimura, H.; Koda, Y. Micropropagation of Cypripedium macranthos var. rebunense through protocorm-like bodies derived from mature seeds. Plant Cell Tissue Organ Cult 78: 273–276; 2004.
Smith, D.L.; Krikorian, A.D. Low external pH replaces 2,4-D in maintaining and multiplying 2,4-D initiated embryogenic cells of carrot. Plant Physiol 72: 329–336; 1990.
Steward, F.C.; Mapes, M.O. Morphogenesis in aseptic cell culture of Cymbidium. Bot Gaz 132: 65–70; 1971.
Van Le, B.; Hang Phuong, N.T.; Anh Hong, L.T.; Tran Thanh Van, K. High frequency shoot regeneration from Rhynchostylis gigantean (orchidaceae) using thin cell layers. Plant Growth Regul 28: 179–185, 1999.
Wimber, D.E. Clonal multiplication of Cymbidium through tissue culture of the shoot meristem. Am Orchid Soc Bull 32: 105–107; 1963.
Ye, X.L.; Cheng, S.J.; Wang, F.X.; Qian, N.F. Morphology of immature seeds and development in vitro of Dendrobium candidum. Acta Bot Yunnanica 10: 285–290; 1988 (In Chinese).
Young, P.S.; Murthy, H.N.; Yoeup, P.K. Mass multiplication of protocorm-like bodies using bioreactor system and subsequent plant regeneration in Phalaenopsis. Plant Cell Tissue Organ Cult 63: 67–72; 2000.
Zhang, Q.X .; Fang, Y.M. Tissue culture and in vitro seedling and protocorm-like body examination of Dendrobium candidum. Acta Bot Boreali-Occidentalia Sinica 25: 1761–1765; 2005 (In Chinese).
Zhao, P.; Wang, W.; Feng, F.S.; Wu, F.; Yang, Z.Q.; High frequency shoot regeneration through transverse thin cell layer culture in Dendrobium candidum Wall ex Lindl. Plant Cell Tissue Organ Cult 90: 131–139; 2007.
Acknowledgments
We thank Emily King for critical reading of the manuscript. This work was supported from the science foundation of Southwest Jiaotong University (X1200511130101).
Author information
Authors and Affiliations
Corresponding author
Additional information
Editor: E. Bunn
Rights and permissions
About this article
Cite this article
Zhao, P., Wu, F., Feng, FS. et al. Protocorm-like body (PLB) formation and plant regeneration from the callus culture of Dendrobium candidum Wall ex Lindl.. In Vitro Cell.Dev.Biol.-Plant 44, 178–185 (2008). https://doi.org/10.1007/s11627-007-9101-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11627-007-9101-2