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Optimization of biotin and thiamine requirements for somatic embryogenesis of date palm (Phoenix dactylifera L.)

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Summary

This study was conducted to examine the effect of biotin and thiamine concentrations on callus growth and somatic embryogenesis of date palm (Phoenix dactylifera L.). Embryogenic callus derived from offshoot tip explants was cultured on hormone-free MS medium containing biotin at 0, 0.1, 1, or 2 mg l−1 combined with thiamine at 0.1, 0.5, 2, or 5 mg l−1. Embryogenic callus weight, number of resultant embryos, and embryo length were significantly influenced by thiamine and biotin concentration. The optimum callus growth treatment consisted of 0.5 mg l−1 thiamine and 2 mg l−1 biotin. This treatment also gave the highest number of embryos. Embryo elongation was greatest at 0.5 or 2 mg l−1 thiamine combined with 1 mg l−1 biotin. Embryos from all treatments germinated and regenerants exhibited normal growth in soil. This study provides an insight into the importance of optimizing various culture medium components to overcome in vitro recalcitrace of date palm.

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  1. Date Palm Research Center, College of Agriculture and Food Sciences, King Faisal University, P.O. Box 400, 31982, Al-Hassa, Saudi Arabia

    Jameel M. Al-Khayri

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  1. Jameel M. Al-Khayri
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Correspondence to Jameel M. Al-Khayri.

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Al-Khayri, J.M. Optimization of biotin and thiamine requirements for somatic embryogenesis of date palm (Phoenix dactylifera L.). In Vitro Cell.Dev.Biol.-Plant 37, 453–456 (2001). https://doi.org/10.1007/s11627-001-0079-x

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  • DOI: https://doi.org/10.1007/s11627-001-0079-x

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