Skip to main content
Log in

Genetic Diversity of Chinese and CIP Potato (Solanum tuberosum L.) Germplasm Assessed by Amplified Fragment Length Polymorphism (AFLP) Markers

  • Published:
Potato Research Aims and scope Submit manuscript

Abstract

The genetic diversity of 123 potato clones, consisting of 103 clonal accessions from the International Potato Center (CIP) and 20 Chinese cultivars, was assessed using amplified fragment length polymorphism (AFLP) markers. Ten polymorphic primer combinations were selected to analyse and compare the diversity of the two sources of clones. A total of 521 reproducible bands were amplified, of which 488 were polymorphic. These AFLP markers were analysed to estimate the genetic distance (GD) and genetic similarity (GS) coefficient of all clones. The GD between pairs of clones ranged from 0.03 to 0.48. Nei’s gene diversity index was between 0.236 and 0.387, with an average of 0.330. The index of Shannon’s information varied from 0.361 to 0.567 with an average of 0.498. A high degree of polymorphism was observed with an average of 93.6% loci found to be polymorphic. Cluster analysis showed that the CIP accessions were grouped together at the GS 0.59 clustering line, whereas most Chinese cultivars grouped at the GS 0.82 clustering line. The diversity in CIP potato resources was found to be higher than that of the Chinese cultivars, indicating that the genetic base of Chinese potato cultivars is narrow and may benefit from broadening.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Anonymous (1989) The cultivation of Chinese potato. China Agricultural Press, Beijing, pp 5–18

    Google Scholar 

  • Bamberg JB, del Rio AH (2008) Proximity and introgression of other potato species does not explain genetic dissimilarity between Solanum verrucosum populations of northern and southern Mexico. Am J Potato Res 85(3):232–238

    Article  Google Scholar 

  • Bert PF, Charmet G, Sourdille P, Hayward MD, Balfourier F (1999) A high-density molecular map for ryegrass (Lolium perenne) using AFLP markers. Theor Appl Genet 99(3–4):445–452

    Article  PubMed  CAS  Google Scholar 

  • Chen ZG, Zeng XW, Zhang T, Deng HC, Su HK (2009) ISSR markers identification of introduced potato varieties. Seed 28(1):83–85

    CAS  Google Scholar 

  • Di H, Chen YL, Jin LP (2006a) Genetic diversity analysis of some Chinese cultivated potato varieties using AFLP markers. Acta Horticulturae Sinica 33(6):1349–1352

    CAS  Google Scholar 

  • Di H, Chen YL, Jin LP (2006b) Genetic diversity analysis of Chinese main potato cultivars by RAPD and AFLP markers. Acta Agron Sin 32(6):899–904

    CAS  Google Scholar 

  • Duan YF, Liu J, Bian CS, Duan SG, Xu JF, Jin LP (2009) Construction of fingerprinting and analysis of genetic diversity with SSR markers for eighty-eight approved potato cultivars (Solanum tuberosum L.) in China. Acta Agron Sin 35(8):1451–1457

    Article  CAS  Google Scholar 

  • Gawel NJ, Jarret RL (1991) A modified CTAB DNA extraction procedure for Musa and Ipomoea. Plant Mol Biol 9(3):262–266

    Article  CAS  Google Scholar 

  • He BD (1979) Introduction and spread of America crops and its effects on gain production of China. World Agric 6:25–31

    Google Scholar 

  • He FF, Yang ZP, Zhang ZS, Wang GX, Wang JC (2007) Genetic diversity analysis of potato germplasm by SRAP markers. J Agric Biotechnol 15(6):1001–1005

    CAS  Google Scholar 

  • Jansky SH, Jin LP, Xie KY, Xie CH, Spooner DM (2009) Potato production and breeding in China. Potato Res 52:57–65

    Article  Google Scholar 

  • Jin LP, Qu DY, Xie KY, Bian CS, Duan SG (2003) Research progress of germplasm resources and breeding of Chinese potato. Seed 5:98–100

    Google Scholar 

  • Kardolus JP, Eck HJ, Berg RG (1998) The potential of AFLPs biosystematics: a first application in Solanum taxonomy (Solanceae). Plant Syst Evol 210:87–103

    Article  Google Scholar 

  • Karp A (2002) The new genetic era: will it help us in managing genetic diversity? In: Engels JMM, Rao V, Brown AHD, Jackson MT (eds) Managing plant genetic diversity. International Plant Genetic Resources Institute, Rome, pp 43–56

    Google Scholar 

  • Kong FL (2007) Quantitative genetics in plants. China Agricultural University Press, Beijing, China

    Google Scholar 

  • Li FD, Wang J, Wang F, Yang YZ, Zhou Y, Sun HH, Ye GJ (2010) Optimization of AFLP reaction system and primer screening for analysis of genetic diversity of potato germplasm. Mol Plant Breed 8(1):179–185

    CAS  Google Scholar 

  • Liang HW, Wang CZ, Li Z, Luo XZ, Zhou GW (2008) Improvement of the silver-stained technique of polyacrylamide gel electrophoresis. Genetic 30(10):1379–1382

    CAS  Google Scholar 

  • Liu FC, Tan XL, Guo HC (2004) Analysis of potato germplasm resources in Yunnan Province by RAPD. Southwest China J Agric Sci 17(2):200–204

    Google Scholar 

  • Ma DC, Yin WL, Luo YQ, Chen M (2008) Genetic diversity of geographic populations of Seabuckthorn carpenter moth: Holcocerus hippophaecolus (Lepidoptera: Cossidae). Beijing Forestry University, Beijing

    Google Scholar 

  • McGregor CE, Van Treuren R, Hoekstra R, Van Hintum TJL (2002) Analysis of the wild potato germplasm of the series Acaulia with AFLPs: implication for ex situ conservation. Theor Appl Genet 104(1):146–156

    Article  PubMed  CAS  Google Scholar 

  • Nei M, Li WH (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci (USA) 76:5269–5273

    Article  CAS  Google Scholar 

  • Prevost A, Wilkinson MJ (1999) A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theor Appl Gen 98(1):107–112

    Article  CAS  Google Scholar 

  • Rohlf FJ (1999) NTSYS-Pc: numerical taxonomy and multivariate analysis system. Version 2.1 Exeter Software, Setauket, NY

  • Teng CC, Zhang YC, Zhang FJ (2009) Qinghai main cultivars of potato genetic diversity of SSR. Mol Plant Breed 7(3):555–561

    CAS  Google Scholar 

  • Tian GK, Li FY, Liu ZJ, Li QQ, Wang LC, Sheng WM, Niu ZM (2007) Genetic diversity analysis of Kexin series potato cultivars by RAPD markers. Chin Agric Sci Bull 23(12):81–84

    CAS  Google Scholar 

  • Tian DC, Zhu HB, Guo JF, Huang JT (2010) Genetic diversity analysis based on potato ISSR. Chin Potato J 24(1):1–5

    Google Scholar 

  • Vos P, Hogers R, Bleeker M et al (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414

    Article  PubMed  CAS  Google Scholar 

  • Xia P (2000) Application of foreign germplasm resources in Chinese potato production. Potato J 14(1):41–43

    Google Scholar 

  • Xiang N (2000) The AFLP technique and its applications for plant study. Plant Physiol Commun 36(3):236–240

    CAS  Google Scholar 

  • Xie KY, Wang Y (2001) CIP potato late blight research in China. J Agric Univ Hebei 24(2):44–48

    CAS  Google Scholar 

  • Xu M (2007) Pedigree and diversity of approved potato cultivars (Solanum tuberosum L.) in China, Thesis for M S, Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Supervisor: Jin L.P

  • Yang ZP, He FF (2007) Genetic diversity of potato (S. tuberosum L.) germplasm resources. Hunan Agricultural University: Hunan

  • Zhai QX (2001) The preliminary research on age of potato introduced into China. Agric Hist China 91–93

  • Zhang LH, Ozias-Akins P, Kochert G, Kochert S, Kresovich S, Dean R, Hanna W (1999) Differentiation of bermuda grass (Cynodon spp.) genotypes by AFLP analyses. Theor Appl Genet 98:895–902

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Jian Wang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wang, F., Li, Fd. & Wang, J. Genetic Diversity of Chinese and CIP Potato (Solanum tuberosum L.) Germplasm Assessed by Amplified Fragment Length Polymorphism (AFLP) Markers. Potato Res. 56, 167–178 (2013). https://doi.org/10.1007/s11540-013-9238-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11540-013-9238-9

Keywords

Navigation