Abstract
Due to their unique plasmonic and optical properties, gold nanorods (GNR) have shown tremendous potential for nano-based applications extending into a variety of fields including bioimaging, sensor development, electronics, and cancer therapy. These distinctive, shape-specific properties are strongly dependent upon the GNR aspect ratio, thus producing the ability to be targeted for an application by fine-tuning their physical parameters. It is owing to their characteristic spectral signature, which is vastly different from that of a cellular setting, that GNRs are emerging as an ideal candidate for nano-based imaging applications. However, one challenge that has emerged in the field of bioimaging is the need to account for the observed plasmon coupling effect that arises from GNR agglomeration in a physiological environment. In this study, GNRs with aspect ratios of 2.5 and 6.0 were actively identified in an in vitro setting through a hyperspectral imaging (HSI) analysis; which successfully recognized and separated the light scattering pattern of these particles from that of the surrounding cells. Through inclusion of agglomerated GNR spectral patterns in the HSI spectral library, this imaging technique was able to overcome the complication of plasmon coupling, though to varying degrees. These results demonstrate the tremendous potential of GNRs coupled with HSI analysis to advance the field of nano-based sensing and imaging mechanisms.
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References
Daniel MC, Astruc D (2004) Gold nanoparticles: assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology. Chem Rev 104:293–346
Panyala NR, Pena-Mendez EM, Havel J (2009) Gold and nano-gold in medicine: overview, toxicology and perspectives. J Appl Biomed 7:75–91
Wang Y, Xie X, Wang X, Ku G, Gill KL, O’Neal DP, Stoica G, Wang LV (2004) Photoacustic tomography of a nanoshell contrast agent in the in vivo rat brain. Nano Lett 4:1689–1692
Lewinski N, Colvin V, Drezek R (2008) Cytotoxicity of nanoparticles. Small 4:26–49
Dreaden EC, Alkilany AM, Huang X, Murphy CJ, El-Sayed MA (2012) The golden age: gold nanoparticles for biomedicine. Chem Soc Rev 41:2740–2779
Murphy CJ, Gole AM, Stone JW, Sisco PN, Alkilany AM, Goldsmith EC, Baxter SC (2008) Gold nanoparticles in biology: beyond toxicity to cellular imaging. Acc Chem Res 41:1721–1730
Goh D, Gong T, Dinish US, Maiti KK, Fu CY, Yong KT, Olivo M (2012) Pluronic triblock copolymer encapsulated gold nanorods as biocompatible localized plasmon resonance-enhanced scattering probes for dark-field imaging of cancer cells. Plasmonics 7:595–601
Link S, El-Sayed MA (1999) Spectral properties and relaxation dynamics of surface plasmon electronic oscillations in gold and silver nanodots and nanorods. J Phys Chem B 103:8410–8426
Garcia MA (2011) Surface plasmons in metallic nanoparticles: fundamentals and applications. J Phys D Appl Phys 44:283001
Huang X, Neretina S, El-Sayed MA (2009) Gold nanorods: from synthesis and properties to biological and biomedical applications. Adv Mat 21:4880–4910
Huang X, El-Sayed IH, Qian W, El-Sayed MA (2006) Cancer cell imaging and photothermal therapy in the near-infrared region by using gold nanorods. J Am Chem Soc 128:2115–2120
Zhu J, Huang L, Zhao J, Wang Y, Zhao Y, Hao L, Lu Y (2005) Shape dependent resonance light scattering properties of gold nanorods. Mat Sci Eng B 121:199–203
Tabor C, Haute DV, El-Sayed MA (2009) Effect of orientation on plasmonic coupling between gold nanorods. ACS Nano 3:3670–3678
Kumar S, Harrison N, Richards-Kortum R, Sokolov K (2007) Plasmonic nanosensors for imaging intracellular biomarkers in live cells. Nano Lett 7:1338–1343
Curry M, Crow J, Wax A (2008) Molecular imaging of epidermal growth factor receptor in live cells with refractive index sensitivity using dark-field microspectroscopy and immunotargeted nanoparticles. J Biomed Opt 13:014022
Wax A, Sokolov K (2009) Molecular imaging and darkfield microspectroscoy of live cells using gold plasmonic nanoparticles. Laser Photonics Rev 3:146–158
Aaron J, Travis K, Harrison N, Sokolov K (2009) Dynamic imaging of molecular assemblies in live cells based on nanoparticle plasmon resonance coupling. Nano Lett 9:3612–3618
Rocha A, Zho Y, Kundu S, Gonzalex JM, BradleighVinson S, Liang H (2011) In vivo observations of gold nanoparticles in the central nervous system of Blaberus discoidalis. J Nanobiotechnology 9:5
Mukhopadhyay A, Grabinski C, Afrooz AR, Saleh NB, Hussain S (2012) Effect of gold nanosphere surface chemistry on protein adsorption and cell uptake in vitro. Appl Biochem Biotechnol 167:327–337
Altinoğlu EI, Adair JH (2010) Near infrared imaging with nanoparticles. WIREs Nanomed Nanobiotechnol 2:461–477
Jana NR, Gearheart LA, Obare SO, Johnson CJ, Edler KJ, Mann S, Murphy CJ (2002) Liquid crystalline assembilies of ordered gold nanorods. J Mat Chem 12:2909–2912
Park K, Vaia RA (2008) Synthesis of complex Au/Ag nanorods by controlled overgrowth. Adv Mat 20:3882–3886
Alkilany AM, Nagaria PK, Hexel CR, Shaw TJ, Murphy CJ, Wyatt MD (2009) Cellular uptake and cytotoxicity of gold nanorods: molecular origin of cytotoxicity and surface effects. Small 5:701–708
Link S, Mohamed MB, El-Sayed MA (1999) Simulation of the optical absorption spectra of gold nanorods as a function of their aspect ratio and the effect of medium dielectric constant. J Phys Chem B 103:3073–3077
Sethi M, Joung G, Knecht MR (2009) Stability and electrostatic assembly of Au nanorods for use in biological assays. Langmuir 25:317–325
Jain PK, Eustis S, El-Sayed MA (2006) Plasmon coupling in nanorod assemblies: optical absorption, discrete dipole approximation simulation, and exciton-coupling model. J Phys Chem B 110:18243–18253
Yu D, Ganta D, Dale E, Rosenberger AT, Wicksted JP, Kalkan AK (2012) Absorption properties of hybrid composites of gold nanorods and functionalized single-walled carbon nanotubes. J Nanomaterials 2012:154278
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This work was funded in part by the Air Force Surgeon General. BMS and KKC received funding through Oak Ridge Institute for Science and Education.
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Stacy, B.M., Comfort, K.K., Comfort, D.A. et al. In Vitro Identification of Gold Nanorods through Hyperspectral Imaging. Plasmonics 8, 1235–1240 (2013). https://doi.org/10.1007/s11468-013-9538-6
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DOI: https://doi.org/10.1007/s11468-013-9538-6