Skip to main content
SpringerLink
Log in

A conductive and biodegradable hydrogel for minimally delivering adipose-derived stem cells

  • Article
  • Published:
Science China Technological Sciences Aims and scope Submit manuscript

Abstract

Injectable hydrogel is one of the most important biomaterials for tissue engineering and drug delivery. However, it is still a challenge to obtain an injectable hydrogel with conductive property on account of the poor water solubility of conductive polymers Here, a conductive hydrogel with controllable biodegradability was constructed for minimally delivering adipose mesenchymal stem cells (ADSCs). Firstly, a disulfide containing and hyperbranched polymer structure poly(β-amino ester) (PBAE) with multi-acrylate end groups was synthesized by poly(ethylene glycol) diacrylate(PEGDA) and cystamine, and then tetraaniline (TA) was grafted on the PBAE chain to obtain a conductive PBAE-TA polymer. PBAE-TA shows a good water solubility, which can be crosslinked by thiol-modified hyaluronic acid (HA-SH) due to the click reaction between acrylate and thiol to in situ form a hydrogel within 1 min. The hydrogel illustrates a good electrical conductivity of 9.6×10−3 S/cm and a controllable biodegradable behavior in dithiothreotol (DTT) solution. PBAE-TA HA-SH hydrogel was subcutaneously injected for delivering ADSCs. The gene expression of Cx43 and TGF-β1 were up-regulated by PBAE-TA/HA-SH hydrogel, suggesting an enhancement in the electrical coupling and an anti-inflammatory property. This injectable, biodegradable, and conductive hydrogel can effectively deliver stem cells, which might be used in skin, muscle, and myocardium regeneration.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Zhao Y J, Zhao K Y, Li Y, et al. Enrichment of Cd2+ from water with a calcium alginate hydrogel filtration membrane. Sci China Tech Sci, 2018, 61: 438–445

    Article  Google Scholar 

  2. Wang Q, Zhang Y Y, Dai X Y, et al. A high strength pH responsive supramolecular copolymer hydrogel. Sci China Tech Sci, 2017, 60: 78–83

    Article  Google Scholar 

  3. Wang H B, Li H F, Wu Y H, et al. A high strength, anti-fouling, self-healable, and thermoplastic supramolecular polymer hydrogel with low fibrotic response. Sci China Tech Sci, 2019, 62: 569–577

    Article  Google Scholar 

  4. Lee J Y, Bashur C A, Goldstein A S, et al. Polypyrrole-coated electrospun PLGA nanofibers for neural tissue applications. Biomaterials, 2009, 30: 4325–4335

    Article  Google Scholar 

  5. Wu Y, Wang L, Guo B, et al. Electroactive biodegradable polyurethane significantly enhanced Schwann cells myelin gene expression and neurotrophin secretion for peripheral nerve tissue engineering. Biomaterials, 2016, 87: 18–31

    Article  Google Scholar 

  6. Deng Z, Guo Y, Zhao X, et al. Multifunctional stimuli-responsive hydrogels with self-healing, high conductivity, and rapid recovery through host-guest interactions. Chem Mater, 2018, 30: 1729–1742

    Article  Google Scholar 

  7. Li X, Rafie A, Smolin Y Y, et al. Engineering conformal nanoporous polyaniline via oxidative chemical vapor deposition and its potential application in supercapacitors. Chem Eng Sci, 2019, 194: 156–164

    Article  Google Scholar 

  8. Wang W, Liang S, Liu W G. Opinion on the recent development of injectable biomaterials for treating myocardial infarction. Sci China Tech Sci, 2017, 60: 1278–1280

    Article  Google Scholar 

  9. Mihardja S S, Sievers R E, Lee R J. The effect of polypyrrole on arteriogenesis in an acute rat infarct model. Biomaterials, 2008, 29: 4205–4210

    Article  Google Scholar 

  10. Green R A, Lovell N H, Poole-Warren L A. Cell attachment functionality of bioactive conducting polymers for neural interfaces. Biomaterials, 2009, 30: 3637–3644

    Article  Google Scholar 

  11. Wang W, Tan B, Chen J, et al. An injectable conductive hydrogel encapsulating plasmid DNA-eNOs and ADSCs for treating myocardial infarction. Biomaterials, 2018, 160: 69–81

    Article  Google Scholar 

  12. Xie M, Wang L, Ge J, et al. Strong electroactive biodegradable shape memory polymer networks based on star-shaped polylactide and aniline trimer for bone tissue engineering. ACS Appl Mater Interfaces, 2015, 7: 6772–6781

    Article  Google Scholar 

  13. Liu Y D, Cui H T, Zhuang X L, et al. Electrospinning of aniline pentamer-graft-gelatin/PLLA nanofibers for bone tissue engineering. Acta Biomater, 2014, 10: 5074–5080

    Article  Google Scholar 

  14. Bao R, Tan B, Liang S, et al. A π-π conjugation-containing soft and conductive injectable polymer hydrogel highly efficiently rebuilds cardiac function after myocardial infarction. Biomaterials, 2017, 122: 63–71

    Article  Google Scholar 

  15. Al Thaher Y, Latanza S, Perni S, et al. Role of poly-beta-amino-esters hydrolysis and electrostatic attraction in gentamicin release from layer-by-layer coatings. J Colloid Interface Sci, 2018, 526: 35–42

    Article  Google Scholar 

  16. Dosta P, Ramos V, Borrös S. Stable and efficient generation of poly(β-amino ester)s for RNAi delivery. Mol Syst Des Eng, 2018, 3: 677–689

    Article  Google Scholar 

  17. Talavera-Pech W A, Esparza-Ruiz A, Quintana-Owen P, et al. Synthesis of pH-sensitive poly(β-amino ester)-coated mesoporous silica nanoparticles for the controlled release of drugs. Appl Nanosci, 2018, 8: 853–866

    Article  Google Scholar 

  18. Xu Q, Guo L, A S, et al. Injectable hyperbranched poly(β-amino ester) hydrogels with on-demand degradation profiles to match wound healing processes. Chem Sci, 2018, 9: 2179–2187

    Article  Google Scholar 

  19. Liu Y, Hu J, Zhuang X, et al. Synthesis and characterization of novel biodegradable and electroactive hydrogel based on aniline oligomer and gelatin. Macromol Biosci, 2012, 12: 241–250

    Article  Google Scholar 

  20. Anderson D G, Akinc A, Hossain N, et al. Structure/property studies of polymeric gene delivery using a library of poly(β-amino esters). Mol Ther, 2005, 11: 426–434

    Article  Google Scholar 

  21. Hawkins A M, Tolbert M E, Newton B, et al. Tuning biodegradable hydrogel properties via synthesis procedure. Polymer, 2013, 54: 4422–4426

    Article  Google Scholar 

  22. Ito T, Yeo Y, Highley C B, et al. The prevention of peritoneal adhesions by in situ cross-linking hydrogels of hyaluronic acid and cellulose derivatives. Biomaterials, 2007, 28: 975–983

    Article  Google Scholar 

  23. Zhao X, Wu H, Guo B, et al. Antibacterial anti-oxidant electroactive injectable hydrogel as self-healing wound dressing with hemostasis and adhesiveness for cutaneous wound healing. Biomaterials, 2017, 122: 34–47

    Article  Google Scholar 

  24. Wang W, Chen J, Li M, et al. Rebuilding postinfarcted cardiac functions by injecting TIIA@PDA nanoparticle-cross-linked ROS-sensitive hydrogels. ACS Appl Mater Interfaces, 2019, 11: 2880–2890

    Article  Google Scholar 

Download references

Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant Nos. 31822020, 51473117, 31771030 & 31870965).

Author information

Authors and Affiliations

  1. Tianjin Key Laboratory of Composite and Functional Materials, School of Materials Science and Engineering, Tianjin University, Tianjin, 300350, China

    YingYing Shang, Wei Liang, BaoYu Tan, Meng Xiao, Yang Zou, WenGuang Liu & Wei Wang

Authors
  1. YingYing Shang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Wei Liang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. BaoYu Tan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Meng Xiao
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yang Zou
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. WenGuang Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Wei Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to WenGuang Liu or Wei Wang.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Shang, Y., Liang, W., Tan, B. et al. A conductive and biodegradable hydrogel for minimally delivering adipose-derived stem cells. Sci. China Technol. Sci. 62, 1747–1754 (2019). https://doi.org/10.1007/s11431-019-9522-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11431-019-9522-4

Keywords

Search

Navigation