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Disposable paper-based bipolar electrode array for multiplexed electrochemiluminescence detection of pathogenic DNAs

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  • Special Topic Analytical Sciences at the Nano-Bio Interface
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Abstract

A novel disposable paper-based bipolar electrode (BPE) array is fabricated for multiplexed electrochemiluminescence (ECL) detection of pathogenic DNAs. This proposed BPE array device consists of 15 units, each consisting of six sensing cells and two reporting cells patterned using hydrophobic wax. A hairpin structure DNA assembled on the cathodes of BPEs hybridizes with Pt nanoparticles (NPs) labeled probe DNA in the presence of complementary target DNA. The introduction of Pt NPs catalyzes the reduction of dissolved O2 at cathodes and induces an enhanced ECL signal from Ru(bpy)3 2+/tripropylamine (TPrA) at the anodes of BPEs. The dissolved O2 lost in reduction reaction could be promptly replenished due to the relatively large contact area of the paper-based cells with air, which ensures the stability of ECL signal. This obtained paper-based BPE array sensor showed excellent performances for the multiplexed analysis of the syphilis (Treponema pallidum) gene, the immunodeficiency virus gene (HIV) and hepatitis B virus gene (HBV).

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Authors and Affiliations

  1. State Key Laboratory of Analytical Chemistry for Life Science; School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, China

    Qiumei Feng, Hongyuan Chen & Jingjuan Xu

Authors
  1. Qiumei Feng
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  2. Hongyuan Chen
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  3. Jingjuan Xu
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Correspondence to Jingjuan Xu.

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Feng, Q., Chen, H. & Xu, J. Disposable paper-based bipolar electrode array for multiplexed electrochemiluminescence detection of pathogenic DNAs. Sci. China Chem. 58, 810–818 (2015). https://doi.org/10.1007/s11426-014-5295-4

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  • DOI: https://doi.org/10.1007/s11426-014-5295-4

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