Abstract
Adult T-cell leukemia/lymphoma (ATL) is a malignancy of mature peripheral T lymphocytes caused by human T-cell lymphotropic virus type I (HTLV-I). In our previous paper, 214 extracts from 162 plants were screened to elucidate the anti-proliferative principles against HTLV-I-infected T-cell lines. In this study, 245 extracts from 182 plants belonging to 61 families were further tested against two HTLV-I-infected T-cell lines (MT-1 and MT-2). Potent anti-proliferative effects were exhibited against MT-1 and MT-2 cells by 52 and 60 of the 245 extracts tested, respectively. Of these, two extracts showed strong inhibitory activity (EC50 values 0.1–1 μg/mL; +++) against both cells, 7 extracts showed moderate inhibitory activity (EC50 values 1–10 μg/mL; ++), and 43 extracts showed weak inhibitory activity (EC50 values 10–100 μg/mL; +), whereas the remaining extracts did not show any activity (EC50 values >100 μg/mL; –) against MT-1 cells. On the other hand, 10 extracts showed moderate inhibitory activit and, 48 extracts showed weak inhibitory activity, whereas the remaining extracts did not show any activity against MT-2 cells. Extracts from the aerial parts of Annona reticulata and A. squamosa showed the most potent inhibitory activity and three aporphine alkaloids were isolated from their extracts as the active principles by activity-guided fractionation.
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Acknowledgments
This work was supported by funds (No.: 106004) from the Central Research Institute of Fukuoka University. We thank Togokusan Fruit Park (Nagoya, Japan) and Hamamatsu Fruit Park (Hamamatsu, Japan) for providing the leaves of the annonaceous plants.
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Nakano, D., Ishitsuka, K., Kamikawa, M. et al. Screening of promising chemotherapeutic candidates from plants against human adult T-cell leukemia/lymphoma (III). J Nat Med 67, 894–903 (2013). https://doi.org/10.1007/s11418-013-0747-2
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DOI: https://doi.org/10.1007/s11418-013-0747-2