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Effect of herbicide used with years (8 + 1) on soil enzymic activity and microbial population diversity

  • Soils, Sec 4 • Ecotoxicology • Research Article
  • Published:
Journal of Soils and Sediments Aims and scope Submit manuscript

Abstract

Purpose

Soil microbes play important roles in plant nutrition and soil conservation, and the diversity and population of soil microbe are influenced by abiotic and biotic factors associated with different soil managements. However, the information concerning soil microbe diversity and population structure and its relation with soil fertility and enzyme activities are scarce in crop rotation under different soil management system.

Materials and methods

This paper reports the effects of three weeding managements (herbicide (2-chloro-N-(ethoxymethyl)-N-(2-ethyl-6-methylphenyl) acetomide, C14H20ClNO2), manual weeding, and no weeding (CK)), on soil microbial diversity, population structure, and its relationship with soil active organic matter (AOM) and pH, and the activity of soil enzymes like sucrase, catalase, and urease activities from long-term test area in red soil upland field in southeast China, which was set up since 2006. Soil samples at 0–20-cm depths were collected before (8 years) and after (8 + 1 years) weeding management in April 2014.

Results and discussion

Soil enzymes (sucrase, catalase, and urease activity) and soil microbial populations had no significant difference (P > 0.05) under the three weeding treatments. Based on richness of microbial population up to 0.10%, the phyla Proteobacteria and Actinobacteria highly dominated the three soil treatments, averagely accounting for 21.76 and 21.44%. Chloroflexi was the next phylum, about accounting for 6.84%. Firmicutes, Verrucomicrobia, and Planctomycetes phylum accounted for 4.98, 4.78, and 4.23%, respectively. The percentage of Gemmatimonadetes was 2.76%, and that of Bacteroidetes was about 1.45%. Armatimonade and Nitrospira were the lowest, with 0.69 and 0.26%, respectively. Among the 20 phyla, only 5 had significant correlation with some of the soil properties. Twenty-one in 46 classes had significant correlation with some of the soil properties. Armatimonadetes and Fusobacteria had positive correlation with moisture. Acidobacteria_Gp3, Deltaproteobacteria, Chthonomonadetes, Armatimonadetes_gp4, and Euryarchaeota also were positively correlated with moisture. Negative correlation between Armatimonadetes, Chloroflexi, Chthonomonadetes, and Armatimonadetes_gp5 and AOM exists, and Armatimonadetes, Chthonomonadetes, Clostridia, Armatimonadetes, and pH were negatively correlated. Fusobacteria was positively correlated with catalase. Acidobacteria_Gp10 and Armatimonadia were positively correlated with catalase. Chthonomonadetes, Clostridia, and Armatimonadetes_gp5 were correlated with urease. Gammaproteobacteria and Flavobacteria were correlated with sucrase.

Conclusions

For long-term herbicide experiment conducted on the Dongxiang upland site, no significant effect of herbicide on soil microbial community composition and enzyme activities was found. Further work is needed to relate microbial community structure and function in different herbicide systems or season sampling, even to detect herbicide effect on community structure during the growing season.

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Acknowledgements

This research was supported by the National Natural Science Foundation of China (31460147), Innovation fund of Jiangxi Academy of Agricultural Sciences (2012CJJ004), program for Cultivating Youths Scientist of Jiangxi Province (2014BCB23010), National Key Technology R&D Program of China (2011BAC13B03, 2011BAC13B04, 2012BAD15B03-02), and by the Opening Fund of Key Laboratory of Poyang Lake Wetland and Watershed Research (Jiangxi Normal University), Ministry of Education (China) (ZK2013001).

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Authors and Affiliations

  1. Jiangxi Provincial Key Lab of Protection and Utilization of Subtropical Plant Resources/Key Laboratory of Poyang Lake Wetland and Watershed Research of Ministry of Education, Jiangxi Normal University, Nanchang, 330022, People’s Republic of China

    Yumei Jiang, Di Lin, Jinfeng Wang, Guangpan Cao & Du Zhu

  2. Institute of Soil and Fertilizer & Resources and Environment, Jiangxi Academy of Agricultural Sciences/Key Laboratory of Crop Ecophysiology and Farming System for the Middle and Lower Reaches of the Yangtze River, Ministry of Agriculture/National Engineering and Technology Research Center for Red Soil Improvement, Nanchang, 330200, People’s Republic of China

    Xianjiao Guan & Chunrui Peng

  3. Jiangxi Science and Technology Normal University, Nanchang, 330038, People’s Republic of China

    Du Zhu

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  1. Yumei Jiang
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  2. Di Lin
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  3. Xianjiao Guan
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  4. Jinfeng Wang
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  6. Du Zhu
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Correspondence to Du Zhu or Chunrui Peng.

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Responsible editor: Qiaoyun Huang

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Jiang, Y., Lin, D., Guan, X. et al. Effect of herbicide used with years (8 + 1) on soil enzymic activity and microbial population diversity. J Soils Sediments 17, 2490–2499 (2017). https://doi.org/10.1007/s11368-017-1672-x

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  • DOI: https://doi.org/10.1007/s11368-017-1672-x

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