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Comparative analysis of the gut microbiota of grass carp fed with chicken faeces

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Abstract

The gut microbiota is closely related to health and disease. Grass carp (Ctenopharyngodon idella) is an important food fish in China. We aimed to investigate the effect of a chicken faeces diet on the gut microbiota composition of grass carp reared in an integrated farming system in China. Gut microbiota compositions of grass carps fed chicken faeces, a commercial diet, and grass were compared based on 16S rRNA gene sequencing. The major intestinal phyla in grass carps fed chicken faeces were Firmicutes, Proteobacteria, and Actinobacteria. The untreated chicken faeces diet altered the gut microbiota composition and increased the number of potential pathogens and antibiotic-resistant bacteria in the gut to varying degrees. To reduce the risk of diseases, it is necessary to remove residual antibiotics and antibiotic-resistant bacteria in chicken faeces by fermentation or other techniques, before it can be used as a fish feed for grass carp.

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Funding

This work was supported by the Scientific Research Foundation for Talent Introduction of Southwest Medical University (0903-00040071) and Science Fund Project of Southwest Medical University (0903-00031532).

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Author notes

  1. Famin Ke and Yanping Gao contributed equally to this work.

Authors and Affiliations

  1. Department of Microbial and Biochemical Pharmacy, School of Pharmacy, Southwest Medical University, No. 319 Section 3, Zhongshan Road, Luzhou, 646000, Sichuan, China

    Famin Ke, Yanping Gao, Li Liu, Chun Zhang, Qin Wang & Xiaowei Gao

  2. State Key Laboratory of New Drug and Pharmaceutical Process, Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, Shanghai, 200437, China

    Li Liu

  3. Department of Medicinal Chemistry, School of Pharmacy, Southwest Medical University, No. 319 Section 3, Zhongshan Road, Luzhou, 646000, Sichuan, China

    Qin Wang

  4. Key Laboratory of Medical Electrophysiology, Ministry of Education, Institute of Cardiovascular Research of Southwest Medical University, Luzhou, 646000, China

    Xiaowei Gao

Authors
  1. Famin Ke
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  2. Yanping Gao
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  3. Li Liu
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  4. Chun Zhang
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  5. Qin Wang
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  6. Xiaowei Gao
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Contributions

XG, CZ, and LL designed the experiments. KF, YG, XG conducted the experiments. XG and QW wrote the manuscript.

Corresponding authors

Correspondence to Qin Wang or Xiaowei Gao.

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The authors declare that they have no conflicts of interest.

Ethical approval

No protected species were involved in this study, and thus, this study did not require specific permissions. The Southwest Medical University Ethics Committee reviewed and approved this study.

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Responsible Editor: Philippe Garrigues

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Ke, F., Gao, Y., Liu, L. et al. Comparative analysis of the gut microbiota of grass carp fed with chicken faeces. Environ Sci Pollut Res 27, 32888–32898 (2020). https://doi.org/10.1007/s11356-020-09012-8

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