Abstract
Seedlings of durum wheat [Triticum turgidum subsp. durum (Desf.) Husn] were exposed to zinc nutrition and to ozone (O3) in a factorial combination: adequate (+Zn treatment) or no Zn (−Zn) in the nutrient solution, followed by exposure to either ozone-free air (filtered air, FA) or to 150 nL L−1 ozone (O3) for 4 h. Although omitting Zn from the nutrient solution failed to impose a genuine Zn deficiency, −Zn*FA durum wheat seedlings showed a typical deficiency behaviour, i.e. Zn mobilisation from root to shoot. Such inter-organ Zn redistribution, however, did not occur in −Zn*O3 plants. Exposure to each stress singly decreased the activity and the protein amount of foliar plasma membrane H+-ATPase, but not stress combination, which even increased the H+-ATPase expression with respect to control. In the −Zn*O3 plants, moreover, the foliar activities of the plasma membrane-bound NAD(P)H-dependent superoxide synthase and of Cu,Zn-superoxide dismutase, and the transcripts abundance of the luminal binding protein and of the protein disulphide isomerase, were also stimulated. It is proposed that, even in the absence of actual Zn starvation, the perception of deficiency conditions could trigger changes in redox homoeostasis at the plasma membrane level, helpful in compensating an O3-dependent oxidative damage.
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Acknowledgments
The authors are greatly indebted to M. I. de Michelis and C. Olivari (anti-H+-ATPase antiserum.), M. L. Guerinot (pZIP cDNA clone), R. Serrano (pMHA2 cDNA clone), L. Leonardo (proteins gel blot analysis), C. Perani (atomic absorption spectroscopy). The University of Tuscia (Intramural Research Grants to MGDB) and the Italian Ministry for University and Research (PRIN Projects Funding No. 2005075991 to MB) are acknowledged for financial support.
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De Biasi, M.G., Marabottini, R., Paolacci, A.R. et al. On the interactions among zinc availability and responses to ozone stress in durum wheat seedlings. Environ Sci Pollut Res 25, 8181–8189 (2018). https://doi.org/10.1007/s11356-017-0062-7
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DOI: https://doi.org/10.1007/s11356-017-0062-7