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Cytotoxicity and oxidative stress responses of silica-coated iron oxide nanoparticles in CHSE-214 cells

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Abstract

The present study aimed at investigating cytotoxicity and oxidative stress induced by silica-coated iron oxide nanoparticles functionalized with dithiocarbamate (Fe3O4 NPs) in Chinook salmon cells (CHSE-214) derived from Oncorhynchus tshawytscha embryos. A significant reduction in cell viability was evident in response to Fe3O4 NPs as revealed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay after 24 h of exposure. Out of the tested concentrations (10, 20, and 30 μg/ml), the highest concentration has shown significant decrease in the viability of cells after 24 h of exposure. Alterations in the morphology of CHSE-214 cells was also evident at 10 μg/ml concentration of Fe3O4 NPs after 24 h. Fe3O4 NPs elicited a significant dose-dependent reduction in total glutathione content (TGSH), catalase (CAT), glutathione reductase (GR) with a concomitant increase in lipid peroxidation (LPO), and protein carbonyl (PC) at highest concentration (30 μg/ml) after 24 h of exposure. In conclusion, our data demonstrated that Fe3O4 NPs have potential to induce cytotoxicity in CHSE-214 cells, which is likely to be mediated through reactive oxygen species generation and oxidative stress.

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Acknowledgments

The authors are grateful to the Portuguese Foundation for Science and Technology (FCT) for post-doctoral grants to KS (SFRH/BPD/79490/2011) and to the Aveiro University Research Institute/CESAM.

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Authors and Affiliations

  1. CESAM-Centre for Environmental and Marine Studies, Department of Chemistry, University of Aveiro, 3810-193, Aveiro, Portugal

    K. Srikanth, Tito Trindade, A. C. Duarte & E. Pereira

  2. Department of Chemistry, CICECO and CESAM, Aveiro Institute of Nanotechnology, University of Aveiro, 3810-193, Aveiro, Portugal

    Tito Trindade

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  1. K. Srikanth
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Correspondence to K. Srikanth.

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Srikanth, K., Trindade, T., Duarte, A.C. et al. Cytotoxicity and oxidative stress responses of silica-coated iron oxide nanoparticles in CHSE-214 cells. Environ Sci Pollut Res 24, 2055–2064 (2017). https://doi.org/10.1007/s11356-016-7870-z

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  • DOI: https://doi.org/10.1007/s11356-016-7870-z

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