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Antibiofouling potential of quercetin compound from marine-derived actinobacterium, Streptomyces fradiae PE7 and its characterization

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Abstract

An attempt has been made to isolate, purify and characterize antifouling compound from Streptomyces fradiae PE7 isolated from Vellar estuarine sediment, Parangipettai, South India. The microbial identification was done at species level based on its phenotypic, cell wall and molecular characteristics. Strain PE7 produced high quantity of antifouling compounds in agar surface fermentation when compared to submerged fermentation. In fermentation optimization, wide range of sugars, amino acids, minerals, pH, temperature and NaCl concentration was found to influence the antifouling compound production from the strain PE7. Antifouling compound PE7-C was purified from the crude extract by preparative TLC, and its activity against biofouling bacteria was confirmed by bioautography. Based on the physico-chemical characteristics, the chemical structure of the antifouling compound PE7-C was identified as quercetin (C15H10O7), a flavonoid class of compound with the molecular weight 302.23 g/mol. The purified quercetin was active against 18 biofouling bacteria with MIC range between 1.6 and 25 μg/ml, algal spore germination and mollusc foot adherence found at 100 μg/ml and 306 ± 19.6 μg ml−1 respectively. The present study, for the first time, reported quercetin from marine-derived Streptomyces sp. PE7 with antifouling activity. This also leads to the repurposing of quercetin for the development of antifouling agent.

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Acknowledgements

The authors are thankful to the Vice-Chancellor and the Registrar of the Periyar University for providing the necessary facilities and our sincere thanks to UGC-MRP (F.No. 37-303/2009 (SR)) for financial assistance through major research project.

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Authors and Affiliations

  1. Centre for Drug Discovery and Development, Sathyabama University, Jeppiar Nagar, Chennai, 600 119, Tamil Nadu, India

    Venugopal Gopikrishnan & Manikkam Radhakrishnan

  2. Actinobacterial Research Laboratory, Department of Microbiology, Periyar University, Periyar Palkalai Nagar, Salem, 636 011, Tamil Nadu, India

    Thangavel Shanmugasundaram, Raasaiyah Pazhanimurugan & Ramasamy Balagurunathan

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  1. Venugopal Gopikrishnan
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  2. Manikkam Radhakrishnan
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  5. Ramasamy Balagurunathan
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Correspondence to Ramasamy Balagurunathan.

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Gopikrishnan, V., Radhakrishnan, M., Shanmugasundaram, T. et al. Antibiofouling potential of quercetin compound from marine-derived actinobacterium, Streptomyces fradiae PE7 and its characterization. Environ Sci Pollut Res 23, 13832–13842 (2016). https://doi.org/10.1007/s11356-016-6532-5

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