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Combined effect of Cd and Pb spiked field soils on bioaccumulation, DNA damage, and peroxidase activities in Trifolium repens

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Abstract

The present study was designed to investigate the combined effects of Cd and Pb on accumulation and genotoxic potential in white clover (Trifolium repens). For this purpose, T. repens was exposed to contaminated soils (2.5–20 mg kg−1 cadmium (Cd), 250–2000 mg kg−1 lead (Pb) and a mixture of these two heavy metals) for 3, 10 and 56 days. The resulting bioaccumulation of Cd and Pb, DNA damage (comet assay) and peroxidase activities (APOX and GPOX) were determined. The exposure time is a determinant factor in experiments designed to measure the influence of heavy metal contamination. The accumulation of Cd or Pb resulting from exposure to the two-metal mixture does not appear to depend significantly on whether the white clover is exposed to soil containing one heavy metal or both. However, when T. repens is exposed to a Cd/Pb mixture, the percentage of DNA damage is lower than when the plant is exposed to monometallic Cd. DNA damage is close to that observed in the case of monometallic Pb exposure. Peroxidase activity cannot be associated with DNA damage under these experimental conditions.

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Acknowledgments

The present study was supported by a grant from ADEME (Agence De l’Environnement et de la Maîtrise de l’Energie) and Anses (Agence nationale de sécurité sanitaire de l’alimentation, de l’environnement et du travail). Fabien Bernard was supported by a doctoral fellowship from ADEME, IRENI (Institut de Recherche en ENvironnement Industriel) and RégionNord/Pas-de-Calais. The authors gratefully thank Dr Franck Marot from ADEME (Agence De l’Environnement et de la Maitrise de l’Energie) for his financial support and helpful discussions.

Conflict of interest

The authors declare that there are no conflicts of interest.

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Authors and Affiliations

  1. Laboratoire des Sciences Végétales et Fongiques (EA 4483), Faculté des Sciences Pharmaceutiques et Biologiques, Université Lille Droit et Santé, F-59006, Lille Cedex, France

    C. Lanier, F. Bernard, S. Dumez, J. Leclercq, I. Devred, D. Cuny & A. Deram

  2. LGCgE (EA 4515), Université Lille Sciences et Technolohgies, Cité scientifique, bâtiment SN3-Rdc, F-59655, Villeneuve d’Ascq, France

    F. Bernard, S. Lemière & F. Vandenbulcke

  3. Laboratoire de Toxicologie Génétique (EA 4483), Institut Pasteur de Lille, 1 rue du Pr Calmette BP245, F-59019, Lille Cedex, France

    F. Nesslany & A. Platel

  4. Faculté Ingénierie et Management de la Santé (EA 4483), Université Lille Droit et Santé, 42 rue Ambroise Paré, F-59120, Loos, France

    C. Lanier & A. Deram

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  1. C. Lanier
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  2. F. Bernard
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  3. S. Dumez
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Correspondence to C. Lanier.

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Lanier, C., Bernard, F., Dumez, S. et al. Combined effect of Cd and Pb spiked field soils on bioaccumulation, DNA damage, and peroxidase activities in Trifolium repens . Environ Sci Pollut Res 23, 1755–1767 (2016). https://doi.org/10.1007/s11356-015-5414-6

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