Abstract
The present study is focused on the effect of chitin derivatives against human cancer cell lines RD and Hep2. As an outcome from this research, chitin was cytotoxic at IC50 = 400 μg/ml and 200 μg/ml against Hep2 cells and RD cells lines, respectively. Irradiated chitin had an IC50 value of 450 μg/ml for Hep2 and an IC50 of 200 μg/ml for RD. The lowest IC50 is attributed to chitosan, 300 μg/ml in Hep2 and 190 μg/ml in RD.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
A.O.A.C. (1990) In: Horritz E (ed) Official methods of analysis (15th ed.). Washington, DC: Association of Official Analytical Chemists, method#930.15 p69, method #942.05 p70, method #962.09 p80
Aspden TJ, Mason JDT, Jones NS, Lowe J, Skaugrud O, Ilum L (1997) Chitosan as nasal delivery systems: the effect of chitosan solutions on in vitro in vivo mucociliary transport rates in human turbinates and volunteers. J Pharm Sci 86:509–513
Baxter A, Dillon M, Taylor KDA, Roberts GAF (1992) Improved method for IR determination of the degree of N-acetylation of chitosan. Inter J Biol Macromol 14:166–169
Benhabiles MS, Abdi N, Drouiche N, Lounici H, Pauss A, Goosen MFA, Mameri N (2012) Protein recovery by ultrafiltration during isolation of chitin from shrimp shells Parapenaeus longirostris. Food Hydrocoll 32(1):28–34
Benhabiles MS, Salah R, Lounici H, Drouiche N, Goosen MFA, Mameri N (2012) Antibacterial activity of chitin, chitosan and its oligomers prepared from shrimp shell waste. Food Hydrocoll 29:48–56
Brugnerotto J, Lizardi J, Goyoolea F, Arguelles-Monal W, Desbrières J, Rinaudo M (2001) An infrared investigation in relation with chitin and chitosan characterization. Polymer 42:3569–3580
Cuesta A, Esteban MA, Meseguer J (2003) Tumouricidal activity of gilthead seabream (Sparus aurata L.) natural cytotoxic cells: the role played in vitro and in vivo by retinol acetate. Fish Shellfish Immunol 14:133–144
Dziril M, Grib H, Laribi-Habchi H, Drouiche N, Abdi N, Lounici H, Pauss A, Mameri N (2015) Chitin oligomers and monomers production by coupling γ radiation and enzymatic hydrolysis. J Ind Eng Chem. doi:10.1016/j.jiec.2014.12.015
Fernandez-Kim SO (2004) Physicochemical and functional properties of crawfish chitosan as affected by different processing protocols. Master Thesis, Department of Food Science. Seoul National University
Fine J (1935) The Biuret method of estimating albumin and globulin in serum and urine. Biochem J 29:799–803
Gamzazade AI, Nasibov SM (2002) Formation and properties of polyelectrolyte complexes of chitosan hydrochloride and sodium dextransulfate. Carbohydr Polym 50:339–343
Hurt AP, Getti G, Coleman NJ (2014) Bioactivity and biocompatibility of a chitosan-tobermorite composite membrane for guided tissue regeneration. Int J Biol Macromol 64:11–16
Kasaai MRA (2008) Review of several reported procedures to determine the degree of N-acetylation for chitin and chitosan using infrared spectroscopy. Carbohydr Polym 71:497–508
Khan TA, Peh KK, Chang HS (2002) Reporting degree of deacetylation of chitosan : the influence of analytical methods. J Pharm Pharm Sci 5:205–212
Kurita K, Tomita K, Tada T, Ishii S, Nishimura SI, Shimoda K (2003) Squid chitin as a potential alternative chitin source: deacetylation behavior and characteristic. J Polym Sci, Part A: Polym Chem 31:485–491
Laribi-Habchi H, Bouanane-Darenfed A, Drouiche N, Pauss A, Mameri N (2015) Purification, characterization, and molecular cloning of an extracellular chitinase from Bacillus licheniformis stain LHH100 isolated from wastewater samples in Algeria. Int J Biol Macromol 72:1117–1128
Lee SJ, Kim SW, Chung H, Park YT, Choi YW, Cho Y-H, Yoon MS (2005) Bioadhesive drug delivery system using glyceryl monooleate for the Intravesical administration of paclitaxel. Chemotherapy 51:311–318
Mirada H, Yaghobi N, Amanpour S, Ahmadi H, Mohagheghi MA, Hormozi F (2002) Preparation of chitosan derived from shrimp’s shell of Persian Gulf as a blood hemostasis agent. Iran Polym J 11:63–68
Mukherjee DP (2001) Method for producing chitin or chitosan. United States Patents, 6,310,188
No HK, Meyers SP (1995) Preparation and characterization of chitin and chitosan: a review. J Aquat Food Prod Technol 4:27–52
Rao MS, Muňoz J, Stevens WF (2000) Critical factors in chitin production by fermentation of shrimp biowaste. Appl Microbiol Biotechnol 54(808–327):813
Sabnis S, Block LH (1997) Improved infrared spectroscopic method for the analysis of degree of N-acetylation of chitosan. Polym Bull 39:67–71
Salah R, Michaud P, Mati F, Harrat Z, Lounici H, Abdi N, Drouiche N, Mameri N (2013) Anticancer activity of chemically prepared shrimp low molecular weight chitin evaluation with the human monocyte leukaemia cell line, THP-1. Int J Biol Macromol 52:333–339
Zemmouri H, Kadouche S, Lounici H, Hadioui M, Mameri N (2011) Use of chitosan in coagulation flocculation of raw water of Keddara and BeniAmrane dams. Water Sci Technol 11:202–210
Author information
Authors and Affiliations
Corresponding author
Additional information
Responsible editor: Philippe Garrigues
Rights and permissions
About this article
Cite this article
Bouhenna, M., Salah, R., Bakour, R. et al. Effects of chitin and its derivatives on human cancer cells lines. Environ Sci Pollut Res 22, 15579–15586 (2015). https://doi.org/10.1007/s11356-015-4712-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11356-015-4712-3