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The cyanobacterium Synechocystis sp. PUPCCC 62: a potential candidate for biotransformation of Cr(VI) to Cr(III) in the presence of sulphate

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Abstract

The cyanobacterium Synechocystis sp., an isolate from polluted water of Satluj river, India, was found resistant to chromium(VI) up to 200 nmol mL−1. In this study, it has been demonstrated that this organism takes up Cr(VI) through a phosphate transporter. The organism removed 250 nmol Cr(VI), 210 nmol phosphate and 180 nmol sulphate mg−1 protein from a buffer solution in 8 h. Cr(VI) uptake by the organism decreased to 135 nmol Cr(VI) removed per milligram protein in the presence of 200 nmol phosphate mL−1, but the same concentration of sulphate did not affect the Cr(VI) uptake. Similarly, the presence of Cr(VI) in the solution affected the phosphate uptake but not sulphate uptake by the test organism. The kinetic studies on Cr(VI) uptake in the presence of phosphate revealed that phosphate and Cr(VI) acted as competitive inhibitors for one another. Phosphate-starved cells of the organism removed more amount of Cr(VI) than the basal medium-grown cells. The uptake of Cr(VI) as well as phosphate by the organism was observed to be a light-dependent process. Cinnamic acid, a phosphate transporter inhibitor, inhibited Cr(VI) uptake by the organism. Results clearly demonstrated that the test organism takes up chromate ions by phosphate transporter and not by the sulphate transporter. This organism is thus a potential candidate for the bioremediation of Cr(VI) from Cr(VI) and sulphate-laden water.

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Acknowledgments

The authors are thankful to the Head of the Department of Botany, and the Coordinator, SAP-II of UGC, Punjabi University, Patiala for laboratory facilities. Shahnaz gratefully acknowledges the financial support in the form of the Maulana Azad National Fellowship by University Grants Commission, New Delhi.

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  1. Department of Botany, Punjabi University, Patiala, 147 002, Punjab, India

    Shahnaz Parveen, J. I. S. Khattar & D. P. Singh

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  1. Shahnaz Parveen
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  2. J. I. S. Khattar
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Correspondence to J. I. S. Khattar.

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Parveen, S., Khattar, J.I.S. & Singh, D.P. The cyanobacterium Synechocystis sp. PUPCCC 62: a potential candidate for biotransformation of Cr(VI) to Cr(III) in the presence of sulphate. Environ Sci Pollut Res 22, 10661–10668 (2015). https://doi.org/10.1007/s11356-015-4260-x

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