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Evaluation of Acinetobacter sp. B9 for Cr (VI) resistance and detoxification with potential application in bioremediation of heavy-metals-rich industrial wastewater

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Abstract

Present work demonstrates Cr (VI) detoxification and resistance mechanism of a newly isolated strain (B9) of Acinetobacter sp. Bioremediation potential of the strain B9 is shown by simultaneous removal of major heavy metals including chromium from heavy-metals-rich metal finishing industrial wastewater. Strain B9 tolerate up to 350 mg L−1 of Cr (VI) and also shows level of tolerance to Ni (II), Zn (II), Pb (II), and Cd (II). The strain was capable of reducing 67 % of initial 7.0 mg L−1 of Cr (VI) within 24 h of incubation, while in presence of Cu ions 100 % removal of initial 7.0 and 10 mg L−1 of Cr (VI) was observed with in 24 h. pH in the range of 6.0–8.0 and inoculum size of 2 % (v/v) were determined to be optimum for dichromate reduction. Fourier transform infrared spectroscopy and transmission electron microscopy studies suggested absorption or intracellular accumulation and that might be one of the major mechanisms behind the chromium resistance by strain B9. Scanning electron microscopy showed morphological changes in the strain due to chromium stress. Relevance of the strain for treatment of heavy-metals-rich industrial wastewater resulted in 93.7, 55.4, and 68.94 % removal of initial 30 mg L−1 Cr (VI), 246 mg L−1 total Cr, and 51 mg L−1 Ni, respectively, after 144 h of treatment in a batch mode.

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Acknowledgments

The financial support provided by University Grants Commission (UGC), Govt. of India in the form of Senior Research Fellowship (SRF) to AB is gratefully acknowledged. We also acknowledge electron microscope facility at All India Institute of Medical Sciences (AIIMS), New Delhi for SEM and Advanced Instrumentation Research Facility at Jawaharlal Nehru University, New Delhi for TEM and FTIR facilities.

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  1. University School of Environment Management, Guru Gobind Singh Indraprastha University, Sector 16-C, Dwarka, New Delhi, 110078, India

    Amrik Bhattacharya & Anshu Gupta

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  1. Amrik Bhattacharya
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  2. Anshu Gupta
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Correspondence to Anshu Gupta.

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Responsible editor: Robert Duran

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Bhattacharya, A., Gupta, A. Evaluation of Acinetobacter sp. B9 for Cr (VI) resistance and detoxification with potential application in bioremediation of heavy-metals-rich industrial wastewater. Environ Sci Pollut Res 20, 6628–6637 (2013). https://doi.org/10.1007/s11356-013-1728-4

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  • DOI: https://doi.org/10.1007/s11356-013-1728-4

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