Abstract
Acetochlor is a widely used herbicide in maize fields; however, the ecological risk of its residue in the soil–plant system remains unknown. We investigated the dissipation dynamics of field dose acetochlor and clarified its impact on microbial biomass and community structure both in the rhizosphere and bulk soil over 1 month after its application. Soil microbial parameters such as quantities of culturable bacteria and fungi represented by colony-forming units, soil microbial biomass carbon (SMBC), and phospholipid fatty acids (PLFAs) were determined across different sampling times. The results showed that the dissipation half-lives of acetochlor were, respectively, 2.8 and 3.4 days in the rhizosphere and bulk soil, and 0.02–0.07 μg/g residual acetochlor could be detected in the soil 40 days after its application. Compared to the bulk soil, microbial communities in the rhizosphere soil were inclined to be affected by the application of acetochlor: SMBC content and bacterial growth were most likely to be increased; however, fungal growth was prone to be inhibited. The principal component analysis of PLFAs, as well as the comparisons of fungi/bacteria and cy17:0/C16:1ω9c ratios between different treatments over sampling time, revealed that the soil microbial community composition was significantly affected by acetochlor at its early application stage (at day 15); thereafter, the effects of acetochlor were attenuated or even could not be detected. Our results suggested that residual acetochlor did not confer a long-term impairment on viable bacterial groups in the rhizosphere and bulk soil.
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References
Bååth E (2003) The use of neutral lipid fatty acids to indicate the physiological conditions of soil fungi. Microb Ecol 45:373–383
Bååth E, Anderson TH (2003) Comparison of soil fungal/bacterial ratios in a pH gradient using physiological and PLFA-based techniques. Soil Biol Biochem 35:955–963
Baran N, Mouvet C, Dagnac T, Jeannot R (2004) Infiltration of acetochlor and two of its metabolites in two contrasting soils. J Environ Qual 33:241–249
Barriuso J, Marín S, Mellado RP (2010) Effect of the herbicide glyphosate on glyphosate-tolerant maize rhizobacterial communities: a comparison with pre-emergency applied herbicide consisting of a combination of acetochlor and terbuthylazine. Environ Microbiol 12:1021–1030
Baudoin E, Benizri E, Guckert A (2001) Metabolic fingerprint of microbial communities from distinct maize rhizosphere compartments. Eur J Soil Biol 37:85–93
Bell JM, Smith JL, Bailey VL, Bolton H (2003) Priming effect and C storage in semi-arid no-till spring crop rotations. Biol Fert Soils 37:237–244
Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Phys 37:911–917
Buyer JS, Kaufman DD (1996) Microbial diversity in the rhizosphere of corn grown under conventional and low-input systems. Appl Soil Ecol 5:21–27
Cai XY, Sheng GY, Liu WP (2007) Degradation and detoxification of acetochlor in soils treated by organic and thiosulfate amendments. Chemosphere 66:286–292
de Vries FT, Hoffland E, van Eekeren N, Brussaard L, Bloem J (2006) Fungal/bacterial ratios in grasslands with contrasting nitrogen management. Soil Biol Biochem 38:2092–2103
Devare M, Londoño RLM, Thies JE (2007) Neither transgenic Bt maize (MON863) nor tefluthrin insecticide adversely affect soil microbial activity or biomass: a 3-year field analysis. Soil Biol Biochem 39:2038–2047
Dictor MC, Baran N, Gautier A, Mouvet C (2008) Acetochlor mineralization and fate of its two major metabolites in two soils under laboratory conditions. Chemosphere 71:663–670
Esperschütz WJ, Buegger F, Winkler JB, Munch JC, Schloter M, Gattinger A (2009) Microbial response to exudates in the rhizosphere of young beech trees (Fagus sylvatica L.) after dormancy. Soil Biol Biochem 41:1976–1985
Feng HM, He HB, Bai Z, Wu YY, Guo BD, Zhang M, Zhang XD (2008) Microbial degradation of acetochlor in mollisol and the effectsof acetochlor on the characteristics of soil phospholipid fatty acids. Ying Yong Sheng Tai Xue Bao 19:1585–1590 (in Chinese)
Foley ME, Sigler V, Gruden CL (2008) A multiphasic characterization of the impact of the herbicide acetochlor on freshwater bacterial communities. ISME J 2:56–66
Frostegård Å, Bååth E (1996) The use of phospholipids fatty acid analysis to estimate bacterial and fungal biomass in soil. Biol Fert Soils 22:59–65
Helgason BL, Walley FL, Germida JJ (2010) Long-term no-till management affects microbial biomass but not community composition in Canadian prairie agroecosytems. Soil Biol Biochem 42:2192–2202
Hiller E, Čerňanský S, Krascsenits Z, Milička J (2009) Effect of soil and sediment composition on acetochlor sorption and desorption. Environ Sci Pollut Res 16:546–554
Jablonkai I (2000) Microbial and photolytic degradation of the herbicide acetochlor. Intern J Environ Anal Chem 78:1–8
Janniche GS, Lindberg E, Mouvet C, Albrechtsen HJ (2010) Mineralization of isoproturon, mecoprop and acetochlor in a deep unsaturated limestone and sandy aquifer. Chemosphere 81:823–831
Janssen PH, Yates PS, Grinton BE, Taylor PM, Sait M (2002) Improved culturability of soil bacteria and isolation in pure culture of novel members of the divisions Acidobacteria, Actinobacteria, Proteobacteria, and Verrucomicrobia. Appl Environ Microbiol 68:2391–2396
Kandeler E, Marschner P, Tscherko D, Gahoonia TS, Nielsen NE (2002) Microbial community composition and functional diversity in the rhizosphere of maize. Plant Soil 238:301–312
Keinanen MM, Korhonen LK, Lehtola MJ (2002) The microbial community structure of drinking water biofilms can be affected by phosphorus availability. Appl Environ Microb 68:434–439
Lengyel Z, Földényi R (2003) Acetochlor as a soil pollutant. Environ Sci Pollut Res 10:13–18
Li XY, Zhang HW, Zhou QX, Su ZC, Zhang CG (2005) Effects of acetochlor and methamidophos on fungal communities in black soils. Pedosphere 15:646–652
Li XY, Zhang HW, Wu MN, Su ZC, Zhang CG (2008) Impact of acetochlor on ammonia-oxidizing bacteria in microcosm soils. J Environ Sci-China 20:1126–1131
Li XY, Su ZC, Li X, Zhang CG, Zhang HW (2010) Assessing the effects of acetochlor on soil fungal communities by DGGE and clone library analysis. Ecotoxicology 19:1111–1116
Liang C, Jesus ED, Duncan DS, Jackson RD, Tiedje JM, Balser TC (2012) Soil microbial communities under model biofuel cropping systems in southern Wisconsin, USA: impact of crop species and soil properties. Appl Soil Ecol 54:24–31
Liphadzi KB, Al-Khatib K, Bensch CN, Stahlman PW, Dille JA, Todd T, Rice CW, Horak MJ (2005) Soil microbial and nematode communities as affected by glyphosate and tillage practices in a glyphosate-resistant cropping system. Weed Sci 53:536–545
Liu SY, Chen YP, Yu HQ, Zhang SJ (2005) Kinetics and mechanisms of radiation-induced degradation of acetochlor. Chemosphere 59:3–19
Lovell RD, Jarvis SC, Bardgett RD (1995) Soil microbial biomass and activity in long-term grassland: effects of management changes. Soil Biol Biochem 27:969–975
Ma QL, Rahman A, Holland PT, James TK, McNaughton DE (2004) Field dissipation of acetochlor in two New Zealand soils at two application rates. J Environ Qual 33:930–938
Marchand AL, Piutti S, Lagacherie B, Soulas G (2002) Atrazine mineralization in bulk soil and maize rhizosphere. Biol Fertil Soils 35:288–292
Martins PF, Carvalho G, Gratão PL, Dourado MN, Pileggi M, Araújo WL, Azevedo RA (2011) Effects of the herbicides acetochlor and metolachlor on antioxidant enzymes in soil bacteria. Process Biochem 46:1186–1195
Marx M, Buegger F, Gattinger A, Zsolnay Á, Munch JC (2007) Determination of the fate of 13C labelled maize and wheat exudates in an agricultural soil during a short-term incubation. Eur J Soil Sci 58:1175–1185
Mijangos I, Becerril JM, Albizu I, Epelde L, Garbisu C (2009) Effects of glyphosate on rhizosphere soil microbial communities under two different plant compositions by cultivation-dependent and -independent methodologies. Soil Biol Biochem 41:505–513
Mills MS, Hill IR, Newcombe AC, Simmons ND, Vaughan PC, Verity AA (2001) Quantification of acetochlor degradation in the unsaturated zone using two novel in situ field techniques: comparisons with laboratory-generated data and implications for groundwater risk assessments. Pest Manag Sci 57:351–359
Nemeth-Konda L, Füleky G, Morovjan G, Csokan P (2002) Sorption behaviour of acetochlor, atrazine, carbendazim, diazinon, imidacloprid and isoproturon on Hungarian agricultural soil. Chemosphere 48:545–552
Ocio JA, Brookes PC (1990) An evaluation of methods for measuring the microbial biomass in soils following recent additions of wheat straw, and the characterization of the biomass that develops. Soil Biol Biochem 22:685–694
Piutti S, Hallet S, Rousseaux S, Philippot L, Soulas G, Martin-laurent F (2002) Accelerated mineralisation of atrazine in maize rhizosphere soil. Biol Fert Soils 36:434–441
Priha O, Grayston SJ, Pennanen T, Smolander A (1999) Microbial activities related to C and N cycling and microbial community structure in the rhizospheres of Pinus sylvestris, Picea abies and Betula pendula seedlings in an organic and mineral soil. FEMS Microbiol Ecol 30:187–199
Puglisi E, Fragoulis G, Ricciuti P, Cappa F, Spaccini R, Piccolo A, Trevisan M, Crecchio C (2009) Effects of a humic acid and its size-fractions on the bacterial community of soil rhizosphere under maize (Zea mays L.). Chemosphere 77:829–837
Ratcliff AW, Busse MD, Shestak CJ (2006) Changes in microbial community structure following herbicide (glyphosate) additions to forest soils. Appl Soil Ecol 34:114–124
Ringelberg DW, Sutton S, White DC (1997) Biomass, bioactivity and biodiversity: microbial ecology of the deep subsurface: analysis of ester-linked phospholipids fatty acids. FEMS Microbiol Rev 20:371–377
Singh N, Megharaj M, Kookana RS, Naidu R, Sethunathan N (2004) Atrazine and simazine degradation in Pennisetum rhizosphere. Chemosphere 56:257–263
Soil Survey Staff (2003) Soil taxonomy: a basic system of soil classification for making and interpreting soil surveys (Agriculture Handbook No. 436). Washington, D.C.: U.S. Government Printing Office
Su ZC, Zhang HW, Li XY, Zhang Q, Zhang CG (2007) Toxic effects of acetochlor, methamidophos and their combination on nifH gene in soil. J Environ Sci-China 19:864–873
Sundh I, Borga P, Milsson M (1995) Estimation of cell numbers of methanotrophic bacteria in boreal peatlands based on analysis of specific phospholipids fatty acids. FEMS Microbiol Ecol 18:103–112
Taylor JP, Wilson B, Mills MS, Burns RG (2002) Comparison of microbial numbers and enzymatic activities in surface soils and subsoils using various techniques. Soil Biol Biochem 34:387–401
Taylor JP, Mills MS, Burns RG (2005) Dissipation of acetochlor and its distribution in surface and sub-surface soil fractions during laboratory incubations. Pest Manag Sci 61:539–548
Vance ED, Brookes PC, Jenkinson DS (1987) An extraction method for measuring soil microbial biomass C. Soil Biol Biochem 19:703–707
Vestal JR, White DC (1989) Lipid analysis in microbial ecology. Bioscience 39:535–541
Virág D, Naár Z, Kiss A (2007) Microbial toxicity of pesticide derivatives produced with UV-photodegradation. B Environ Contam Tox 79:356–359
Weaver MA, Krutz LJ, Zablotowicz RM, Reddy KN (2007) Effects of glyphosate on soil microbial communities and its mineralization in a Mississippi soil. Pest Manag Sci 63:388–393
Wu MN, Zhang XL, Zhang HW, Zhang Y, Li XY, Zhou QX, Zhang CG (2009) Soil pseudomonas community structure and its antagonism towards Rhizoctonia solani under the stress of acetochlor. B Environ Contam Tox 83:313–317
Xiao NW, Jing BB, Ge F, Liu XH (2006a) The fate of herbicide acetochlor and its toxicity to Eisenia fetida under laboratory conditions. Chemosphere 62:1366–1373
Xiao NW, Song Y, Ge F, Liu XH, Ou-Yang ZY (2006b) Biomarkers responses of the earthworm Eisenia fetida to acetochlor exposure in OECD soil. Chemosphere 65:907–912
Xu J, Yang M, Dai J, Cao H, Pan C, Qiu X, Xu M (2008) Degradation of acetochlor by four microbial communities. Bioresource Technol 99:7797–7802
Ye C (2003) Environmental behavior of the herbicide acetochlor in soil. B Environ Contam Tox 71:919–923
Yoshitomi KJ, Shann JR (2001) Corn (Zea mays L.) root exudates and their impact on 14C-pyrene mineralization. Soil Biol Biochem 33:1769–1776
Zabaloy MC, Garland JL, Gómez MA (2008) An integrated approach to evaluate the impacts of the herbicides glyphosate, 2,4-D and metsulfuron-methyl on soil microbial communities in the Pampas region, Argentina. Appl Soil Ecol 40:1–12
Zelles L (1999) Fatty acid patterns of phospholipids and lipopolysaccharides in the characterisation of microbial communities in soil: a review. Biol and Fert Soils 29:111–129
Zhang HW, Zhang Q, Zhou QX, Zhang CG (2003) Binary-joint effects of acetochlor, methamidophos, and copper on soil microbial population. B Environ Contam Tox 71:746–754
Acknowledgments
The authors would like to acknowledge the financial support for this study as research projects of the National Natural Science Foundation of China (nos. 41130524 and 41071161). We thank two anonymous reviewers for their great effort in improving the original manuscript.
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Bai, Z., Xu, HJ., He, HB. et al. Alterations of microbial populations and composition in the rhizosphere and bulk soil as affected by residual acetochlor. Environ Sci Pollut Res 20, 369–379 (2013). https://doi.org/10.1007/s11356-012-1061-3
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DOI: https://doi.org/10.1007/s11356-012-1061-3