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High-density linkage maps for Citrus sunki and Poncirus trifoliata using DArTseq markers

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Abstract

The construction of a high-resolution genetic map of citrus would be of great value to breeders and to associate genomic regions with characteristics of agronomic interest. Here, we describe a novel high-resolution map of citrus using a population derived from a controlled cross between Citrus sunki (female parent) and Poncirus trifoliata (male parent). The genetic linkage maps were constructed using DArTseq markers and a pseudo-testcross strategy; only markers showing the expected segregation ratio were considered. To investigate synteny, all markers from both linkage maps were aligned with the genome of Citrus sinensis. The C. sunki map has a total of 2778 molecular markers and a size of 2446.6 cM, distributed across ten linkage groups. The map of P. trifoliata was built with 3084 markers distributed in a total of nine linkage groups, with a total size of 2411.6 cM. These maps are the most saturated linkage maps available for C. sunki and P. trifoliata and have high genomic coverage. We also demonstrated that the maps reported here are closely related to the reference genome of C. sinensis.

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Funding

This study was carried out with the financial support of the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) (processes no. 11/18605-0 and 2014/50880-0) and Instituto Nacional de Ciência e Tecnologia (INCT) de Genômica para Melhoramento de Citros (process no. 573848/08-4).

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Authors and Affiliations

  1. Centro APTA Citros Sylvio Moreira, Instituto Agronômico (IAC), CP 04, Cordeirópolis, SP, 13490-970, Brazil

    Maiara Curtolo, Tatiany Aparecida Teixeira Soratto, Marco Aurélio Takita, Marcos Antonio Machado & Mariângela Cristofani-Yaly

  2. Cidade Universitária Zeferino Vaz, Universidade Estadual de Campinas (Unicamp), Rua Monteiro Lobato, 255, Campinas, SP, 13083-862, Brazil

    Maiara Curtolo

  3. Centro de Ciências Agrárias, Universidade Federal de São Carlos (UFSCar), Rodovia Anhanguera, km 174, Araras, SP, 13600-970, Brazil

    Tatiany Aparecida Teixeira Soratto & Rodrigo Gazaffi

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  1. Maiara Curtolo
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  2. Tatiany Aparecida Teixeira Soratto
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  3. Rodrigo Gazaffi
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  4. Marco Aurélio Takita
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  5. Marcos Antonio Machado
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  6. Mariângela Cristofani-Yaly
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Correspondence to Mariângela Cristofani-Yaly.

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Communicated by C. Chen

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Curtolo, M., Soratto, T.A.T., Gazaffi, R. et al. High-density linkage maps for Citrus sunki and Poncirus trifoliata using DArTseq markers. Tree Genetics & Genomes 14, 5 (2018). https://doi.org/10.1007/s11295-017-1218-9

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  • DOI: https://doi.org/10.1007/s11295-017-1218-9

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