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Pyramiding of male-sterile genes in Cryptomeria japonica D. Don with the aid of closely linked markers

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Abstract

Gene pyramiding is a breeding method used to combine multiple useful genes. Although several genes have been pyramided in certain crops, gene pyramiding has not previously been applied to forest trees. In this study, we used the markers closely linked to the two male-sterile genes MS1 and MS2 for the effective development of individuals doubly heterozygous for these two genes. This is the first example of gene pyramiding through marker-assisted selection (MAS) in forest trees. The markers gSNP06239, which is closely linked to the MS1 gene, and estSNP00695, which is closely linked to MS2, were used in MAS. On the basis of the linkage phase between the markers and male-sterile loci, we selected five F1 individuals (S3-64 from Shindai-3 × Kamikiri-31, S3-70 from Shindai-3 × Kamikiri-38, S3-77 from Shindai-3 × Kamikiri-47, S1-22 from Shindai-1 × Nakakubiki-4, and S1-56 from Shindai-1 × Setsugai-20) as parents for artificial crossing. The 268 seedlings obtained from six artificial cross combinations were used in this study. Chi-squared tests showed no significant deviation from the expected Mendelian ratios of genotypes, indicating that MAS using markers closely linked to the male-sterile genes worked very well. Fifteen individuals that showed unexpected genotypes were probably recombinants, because the map distances between the male-sterile locus and the DNA markers were 4.1 cM (gSNP06239 to MS1) and 6.9 cM (estSNP00695 to MS2). Development of markers more closely linked to the male-sterile loci will facilitate precise gene pyramiding in the future.

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Acknowledgements

The authors would like to thank M. Koshiba, Y. Komatsu, M. Wakao, and T. Tadama for assistance with laboratory work and the sampling of leaves. We also thank Y. Sato for artificial crossing. This work was supported by a Science and Technology Research Promotion Program for Agriculture, Forestry, Fisheries, and Food Industry.

Data archiving statement

The information for SNP markers used in this study were deposited into DDBJ (accession numbers: AB760463-760466 and AB890286-890289).

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Authors and Affiliations

  1. Graduate School of Science and Technology, Niigata University, 8050, Igarashi 2-Nocho, Nishi-ku, Niigata, 950-2181, Japan

    Yoshinari Moriguchi

  2. Niigata Prefectural Forest Research Institute, 2249-5 Unotoro, Murakami, Niigata, 958-0264, Japan

    Satoko Totsuka & Junji Iwai

  3. Department of Forest Genetics, Forestry and Forest Products Research Institute, 1 Matsunosato, Tsukuba, Ibaraki, 305-8687, Japan

    Asako Matsumoto & Saneyoshi Ueno

  4. Faculty of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki, 305-8572, Japan

    Yoshihiko Tsumura

Authors
  1. Yoshinari Moriguchi
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  2. Satoko Totsuka
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  3. Junji Iwai
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  4. Asako Matsumoto
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  5. Saneyoshi Ueno
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  6. Yoshihiko Tsumura
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Corresponding author

Correspondence to Yoshinari Moriguchi.

Additional information

Communicated by P. Ingvarsson

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Moriguchi, Y., Totsuka, S., Iwai, J. et al. Pyramiding of male-sterile genes in Cryptomeria japonica D. Don with the aid of closely linked markers. Tree Genetics & Genomes 13, 61 (2017). https://doi.org/10.1007/s11295-017-1149-5

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  • DOI: https://doi.org/10.1007/s11295-017-1149-5

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