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Comparative analysis of Pinus pinea and Pinus pinaster dehydrins under drought stress

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Abstract

Maritime pine (Pinus pinaster) and stone pine (P. pinea) are two of the most drought-resistant Mediterranean trees. Several studies have analysed the response to drought stress at the molecular level in maritime pine, including the identification of drought-induced genes, transcriptomic analysis during dehydration or in-depth characterisation and diversity studies of specific candidate genes. On the contrary, much less information is available for stone pine, and notwithstanding being a closely related species, significant differences in the transcription profile of several genes during drought, evaluated using microarrays, were reported recently for these two species. In this study, we focus on P. pinea dehydrins, one of the most important gene families expressed in response to drought. We have identified eight dehydrin genes in P. pinea, orthologous to the ones previously described in P. pinaster, and have compared their transcription profiles under drought stress. For this purpose, we imposed a severe and prolonged drought treatment to P. pinea seedlings and analysed the expression pattern of proteins from the dehydrin family in needles, stems and roots. The complete open reading frames of these genes were amplified from cDNA and genomic DNA, and their intron/exon structures were determined. qRT-PCR was performed to analyse their expression pattern in needles, stems and roots during the drought treatment. Remarkable differences between the two species have been detected in the transcript patterns of five out of the eight genes, which could be related with the different behaviours described for these species under drought stress.

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Acknowledgments

The authors would like to thank Dr. Martin Venturas, as well as the two anonymous reviewers and the editor for their helpful suggestions on the manuscript. This work has been funded through the projects AGL2006-03242/FOR (Spanish Ministry of Education and Science), CCG07-UPM/AMB-1932 and CCG10-UPM/AMB-5038 (Madrid Regional Government-UPM).

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I state that this work is all original research carried out by the authors. All authors agree with the contents of the manuscript and its submission to the journal. No part of the research has been published in any form elsewhere. The manuscript is not being considered for publication elsewhere whilst it is being considered for publication in this journal. Any research in the paper not carried out by the authors is fully acknowledged in the manuscript. All sources of funding are acknowledged in the manuscript. The authors declare that they have no competing interests.

Data archiving

The sequences of P. pinea dehydrin genes obtained in this study were submitted to GenBank with the following accessions numbers: KM033825–KM033832 for genomic DNA and KM033836–KM033838, KM033840–KM033842 for mRNA.

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  1. GENFOR Grupo de investigación en Genética y Fisiología Forestal, Universidad Politécnica de Madrid, Ciudad Universitaria s/n, 28040, Madrid, Spain

    Pedro Perdiguero, Álvaro Soto & Carmen Collada

  2. Unidad Mixta de Genómica y Ecofisiología Forestal, INIA/UPM, Madrid, Spain

    Pedro Perdiguero, Álvaro Soto & Carmen Collada

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  1. Pedro Perdiguero
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  2. Álvaro Soto
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Correspondence to Pedro Perdiguero.

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Communicated by S. C. González-Martínez

This article is part of the Topical Collection on Genome Biology

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Perdiguero, P., Soto, Á. & Collada, C. Comparative analysis of Pinus pinea and Pinus pinaster dehydrins under drought stress. Tree Genetics & Genomes 11, 70 (2015). https://doi.org/10.1007/s11295-015-0899-1

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