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Different behavior of Staphylococcus epidermidis in intracellular biosynthesis of silver and cadmium sulfide nanoparticles: more stability and lower toxicity of extracted nanoparticles

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Abstract

Chemical reagents that are used for synthesis of nanoparticles are often toxic, while biological reagents are safer and cost-effective. Here, the behavior of Staphylococcus epidermidis (ATCC 12228) was evaluated for biosynthesis of silver nanoparticles (Ag-NPs) and cadmium sulfide nanoparticles (CdS-NPs) using TEM images intra- and extracellularly. The bacteria only biosynthesized the nanoparticles intracellularly and distributed Ag-NPs throughout the cytoplasm and on outside surface of cell walls, while CdS-NPs only formed in cytoplasm near the cell wall. A new method for purification of the nanoparticles was used. TEM images of pure CdS-NPs confirmed biosynthesis of agglomerated nanoparticles. Biosynthetic Ag-NPs were more stable against bright light and aggregation reaction than synthetic Ag-NPs (prepared chemically) also biosynthetic Ag-NPs displayed lower toxicity in in vitro assays. CdS-NPs indicated no toxicity in in vitro assays. Biosynthetic nanoparticles as product of the detoxification pathway may be safer and more stable for biosensors.

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Acknowledgments

This research was supported financially (No. 931498) by the Biotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences (MUMS), Mashhad, Iran.

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Authors and Affiliations

  1. Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

    Zohreh Rezvani Amin & Bibi Sedigheh Fazly Bazzaz

  2. Department of Pharmaceutical Control, School of Pharmacy, Mashhad University of Medical Sciences, P.O. Box 91775-1365, Mashhad, Iran

    Zahra Khashyarmanesh & Bibi Sedigheh Fazly Bazzaz

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  1. Zohreh Rezvani Amin
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Correspondence to Bibi Sedigheh Fazly Bazzaz.

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Rezvani Amin, Z., Khashyarmanesh, Z. & Fazly Bazzaz, B.S. Different behavior of Staphylococcus epidermidis in intracellular biosynthesis of silver and cadmium sulfide nanoparticles: more stability and lower toxicity of extracted nanoparticles. World J Microbiol Biotechnol 32, 140 (2016). https://doi.org/10.1007/s11274-016-2110-8

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