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Cyclic dipeptides produced by fungus Eupenicillium brefeldianum HMP-F96 induced extracellular alkalinization and H2O2 production in tobacco cell suspensions

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Abstract

Extracellular alkalinization and H2O2 production are important early events during induced systemic resistance (ISR) establishment in plants. In a screen for metabolites as potential ISR activators from 98 fungal isolates associated with marine sponge Hymeniacidon perleve, the crude metabolites of fungus Eupenicillium brefeldianum HMP-F96 induced significant extracellular alkalinization coupled with H2O2 production in tobacco cell suspensions. A combined bioactivity and 1H NMR-guided fractionation approach was used to disclose the chemical determinants responsible for the activities. Eight cyclic dipeptides were purified from the fermentation broth of the strain and were structurally characterized by NMR and MS experiments. This study represents the first report of the occurrence of cyclic dipeptides in E. brefeldianum and of their activities of inducing extracellular alkalinization and H2O2 production in tobacco cell suspensions.

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Acknowledgments

This study was financially supported by National Natural Science Foundation of China (31471814), National High Technology Research and Development Program of China (863 Program, 2011AA09070404) and the National Science and Technology Pillar Program (2011BAE06B04).

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Authors and Affiliations

  1. Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, 110016, China

    Xiaoqi Chen, Nan Wang, Xiao Wang & Jiangchun Hu

  2. University of Chinese Academy of Sciences, Beijing, 100049, China

    Xiaoqi Chen

  3. Shenyang Pharmaceutical University, Shenyang, 110016, China

    Yanhua Mou, Junhong Ling & Xiao Wang

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  1. Xiaoqi Chen
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  2. Yanhua Mou
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  4. Nan Wang
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Correspondence to Nan Wang.

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Chen, X., Mou, Y., Ling, J. et al. Cyclic dipeptides produced by fungus Eupenicillium brefeldianum HMP-F96 induced extracellular alkalinization and H2O2 production in tobacco cell suspensions. World J Microbiol Biotechnol 31, 247–253 (2015). https://doi.org/10.1007/s11274-014-1759-0

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