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Assessment of functional and genetic diversity of aerobic endospore forming Bacilli from rhizospheric soil of Phyllanthus amarus L.

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Abstract

Fifty two aerobic and endospore forming Bacilli (AEFB) strains were recovered from rhizospheric soil of Phyllanthus amarus. Morphological, biochemical and molecular characterization by 16S rDNA gene sequencing has shown that these bacterial strains belong to six different genera of AEFB i.e. Bacillus, Brevibacillus, Lysinibacillus, Paenibacillus, Terribacillus and Jeotgalibacillus. Analysis of their PGP activities has shown that 92.30 % strains produced indole acetic acid hormone, 86.53 % of the strains solubilized Phosphate and 44.23 % strains produced siderophore. Chitinase production activity was shown by 42.30 % of the strains and 21.15 % of the strains produced 1-amino cyclopropane-1-carboxylate (ACC) deaminase. 46.15 % of isolates have shown antagonistic activity against common fungal pathogen of the plant i.e. Corynespora cassiicola. Among all of the isolated strains B. Cereus JP44SK22 and JP44SK42 have shown all of the six plant growth promoting traits tested. B. megaterium strains (JP44SK18 and JP44SK35), Lysinibacillus sphaericus strains (JP44SK3 and JP44SK4) and Brevibacillus laterosporus strain JP44SK51 have also shown multiple PGP activities except ACC deaminase production activity. In the present study bacterial strain belonging to genera Jeotgalibacillus sp. JP44SK37 has been reported first time as a member of rhizospheric soil habitat and has also shown PGP activities. It can be concluded that Rhizosphere of P. amarus has harboured a good diversity of AEFB bacterial strains having a lot of biofertilizing and biocontrol abilities.

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Acknowledgments

Council of Scientific and industrial Research (CSIR), India is acknowledged to provide Senior Research Fellowship to author Sangeeta Kadyan. Authors are also thankful to UGC, New Delhi for the award of UGC-SAP grant and Department of Science and Technology, New Delhi for providing financial grants under DST-FIST programme.

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  1. Department of Genetics, M. D. University, Rohtak, 124001, Haryana, India

    Sangeeta Kadyan, Manju Panghal, Sandeep Kumar, Khushboo Singh & Jaya Parkash Yadav

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  1. Sangeeta Kadyan
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  2. Manju Panghal
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  3. Sandeep Kumar
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Correspondence to Jaya Parkash Yadav.

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11274_2013_1323_MOESM1_ESM.jpg

ESM Fig. 1: Colony morphology pictures of 52 AEFB strains (A to AZ) B. marisflavi JP44SK40, B. megaterium JP44SK1, B. megaterium JP44SK2, Lysinibacillus sphaericus JP44SK3, L. sphaericus JP44SK4, B. flexus JP44SK19, B. megaterium strain JP44SK21, B. firmus JP44SK20, Brevibacillus laterosporus JP44SK51, B. cereus JP44SK22, B. aryabhattai JP44SK38, B. megaterium JP44SK39, Brevibacillus laterosporus JP44SK41, B. megaterium JP44SK5, B. licheniformis JP44SK6, Paenibacillus sp. JP44SK7, B. mycoides JP44SK8, B. mycoides JP44SK9, B. subtilis subsp. spizizenii JP44SK23, B. subtilis subsp. spizizenii JP44SK24, B. simplex JP44SK25, B. simplex JP44SK26, B. cereus JP44SK27, B. cereus JP44SK42, B. cereus JP44SK43, Jeotgalibacillus sp. JP44SK56, B. aryabhattai JP44SK11, B. megaterium JP44SK10, Lysinibacillus xylanilyticus JP44SK52, B. simplex JP44SK12, B. simplex JP44SK13, B. aquimaris JP44SK28, B. simplex JP44SK29, B. simplex JP44SK30, B. simplex JP44SK31, B. simplex JP44SK32, B. cereus JP44SK44, B. cereus JP44SK45, Terribacillus saccharophilus JP44SK46, B. arsenicus JP44SK14, B. marisflavi JP44SK15, B. firmus JP44SK16, Bacillus sp. JP44SK17, B. megaterium JP44SK18, B. cereus JP44SK34, B. cereus JP44SK33, B. megaterium JP44SK35, B. mycoides JP44SK36, B. cereus JP44SK37, Terribacillus goriensis JP44SK47, B. cereus JP44SK49, B. mycoides JP44SK50 (JPEG 229 kb)

ESM Fig. 2(A, B): Antagonistic activity pictures of six bacterial strains (JPEG 45 kb)

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Kadyan, S., Panghal, M., Kumar, S. et al. Assessment of functional and genetic diversity of aerobic endospore forming Bacilli from rhizospheric soil of Phyllanthus amarus L.. World J Microbiol Biotechnol 29, 1597–1610 (2013). https://doi.org/10.1007/s11274-013-1323-3

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