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Biodegradation of chlorpyrifos by bacterial consortium isolated from agriculture soil

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Abstract

Organophosphorous pesticides are widely used in agriculture to control major insect pests. Chlorpyrifos is one of the major organophosphorous pesticides which is used to control insects including termites, beetles. The widespread use of these pesticides is hazardous to the environment and also toxic to mammals, thus it is essential to remove the same from the environment. From the chlorpyrifos contaminated soil nine morphologically different bacterial strains, one actinomycete and two fungal strains were isolated. Among those isolates four bacterial strains which were more efficient were developed as consortium. The four bacterial isolates namely Pseudomonas putida (NII 1117), Klebsiella sp., (NII 1118), Pseudomonas stutzeri (NII 1119), Pseudomonas aeruginosa (NII 1120) present in the consortia were identified on the basis of 16S rDNA analysis. The intracellular fractions of the consortium exhibited more organophosphorus hydrolase activity (0.171 ± 0.003 U/mL/min). The degradation studies were carried out at neutral pH and temperature 37°C with chlorpyrifos concentration 500 mg L−1. LC-mass spectral analysis showed the presence of metabolites chlopyrifos-oxon and Diethylphosphorothioate. These results highlight an important potential use of this consortium for the cleanup of chlorpyrifos contaminated pesticide waste in the environment.

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Fig. 1
Fig. 2

Abbreviations

CP:

Chlorpyrifos

TCP:

3,5,6-trichloro-2-pyridinol

OPH:

Organophosphorous hydrolase

OPs:

Organophosphates

MSM:

Minimal salt medium

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Acknowledgments

The authors are thankful to Dr. T. Kumar Mass spectra analyst for the interpretation of mass spectra results and the facilities provided by SRM University to support this work.

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Authors and Affiliations

  1. Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, 603203, India

    Sonia Jiwal & Pallabi Rout

  2. Department of Genetic Engineering, School of Bioengineering, SRM University, Kattankulathur, 603203, India

    Chitrambalam Sasikala & Mohandass Ramya

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  1. Chitrambalam Sasikala
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  2. Sonia Jiwal
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  3. Pallabi Rout
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  4. Mohandass Ramya
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Correspondence to Mohandass Ramya.

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Sasikala, C., Jiwal, S., Rout, P. et al. Biodegradation of chlorpyrifos by bacterial consortium isolated from agriculture soil. World J Microbiol Biotechnol 28, 1301–1308 (2012). https://doi.org/10.1007/s11274-011-0879-z

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  • DOI: https://doi.org/10.1007/s11274-011-0879-z

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