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Actinomycetes isolated from medicinal plant rhizosphere soils: diversity and screening of antifungal compounds, indole-3-acetic acid and siderophore production

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Abstract

A total of 445 actinomycete isolates were obtained from 16 medicinal plant rhizosphere soils. Morphological and chemotaxonomic studies indicated that 89% of the isolates belonged to the genus Streptomyces, 11% were non-Streptomycetes: Actinomadura sp., Microbispora sp., Micromonospora sp., Nocardia sp, Nonomurea sp. and three isolates were unclassified. The highest number and diversity of actinomycetes were isolated from Curcuma mangga rhizosphere soil. Twenty-three Streptomyces isolates showed activity against at least one of the five phytopathogenic fungi: Alternaria brassicicola, Collectotrichum gloeosporioides, Fusarium oxysporum, Penicillium digitatum and Sclerotium rolfsii. Thirty-six actinomycete isolates showed abilities to produce indole-3-acetic acid (IAA) and 75 isolates produced siderophores on chrome azurol S (CAS) agar. Streptomyces CMU-PA101 and Streptomyces CMU-SK126 had high ability to produced antifungal compounds, IAA and siderophores.

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Acknowledgments

This work was supported by The Royal Golden Jubilee Ph.D. Program (PHD/0153/2546). We are grateful to Dr. Eric H. C McKenzie (Landcare Research, Private Bag 92170, Auckland, New Zealand) for improving the English text.

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Authors and Affiliations

  1. Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand

    Sutthinan Khamna & Saisamorn Lumyong

  2. Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo, Japan

    Akira Yokota

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  1. Sutthinan Khamna
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  2. Akira Yokota
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  3. Saisamorn Lumyong
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Correspondence to Saisamorn Lumyong.

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Khamna, S., Yokota, A. & Lumyong, S. Actinomycetes isolated from medicinal plant rhizosphere soils: diversity and screening of antifungal compounds, indole-3-acetic acid and siderophore production. World J Microbiol Biotechnol 25, 649–655 (2009). https://doi.org/10.1007/s11274-008-9933-x

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  • DOI: https://doi.org/10.1007/s11274-008-9933-x

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