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Acute Toxicity of Metaldehyde in the Invasive Rice Snail Pomacea canaliculata and Sublethal Effects on Tadpoles of a Non-target Species (Rhinella arenarum)

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Abstract

We investigated the effects of exposure to molluscicidal metaldehyde (MET) on golden apple snail (GAS) Pomacea canaliculata and Rhinella arenarum tadpoles by assessing mortality and/or other effects via: acute toxicity assays; B-esterase activities (acetilcholinesterase (AChE) and carboxilesterase (CbE)) and oxidative responses (glutathione-S-transferase (GST) and catalase (CAT)). The effect of sublethal concentrations of MET was also analysed by assessing biochemical changes and swimming parameters in tadpoles. The LC50 value in P. canaliculata was as 0.50 mg L−1 and in R. arenarun tadpoles, 229.7 mg L−1 at 48 h. The intestine of MET-exposed P. canaliculata exhibited a significant reduction in CbE and CAT activities, but not in AChE activity; hepatopancreas of GAS showed a decreased GST activity decreased with respect to control individuals. In addition, a significant reduction of CbE activities was detected in R. arenarum tadpoles exposed to MET, and AChE presented lower values than the control but without statistical differences. Antioxidant enzymes (GST and CAT) were significantly reduced in tadpoles exposed to MET compared with the control group. In addition, MET had a significant effect on the swimming behaviour of R. arenarum. Finally, since amphibian tadpoles and P. canaliculata often co-occur, other native amphibian species should be studied to elucidate the ecological risk of MET to amphibian populations.

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Acknowledgements

This study was supported in part by Secretaría de Ciencia y Tecnología (SECYT), Curso de Acción para la Investigación y Desarrollo (Programa de I+D Orientado a Problemas Sociales y Productivos, UNL) and Agencia Santafesina de Ciencia, Tecnología e Innovación (ASaCTeI).

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  1. Ecotoxicology Laboratory, Faculty of Biochemistry and Biological Sciences, National University of Littoral (FBCB-UNL), Paraje el Pozo s/n, 3000, Santa Fe, Argentina

    Andrés Maximiliano Attademo, Rafael Carlos Lajmanovich, Paola Mariela Peltzer & Celina María Junges

  2. National Council for Scientific and Technical Research (CONICET-FBCB-UNL), Paraje el Pozo s/n, 3000, Santa Fe, Argentina

    Andrés Maximiliano Attademo, Rafael Carlos Lajmanovich, Paola Mariela Peltzer & Celina María Junges

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Correspondence to Andrés Maximiliano Attademo.

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Animals used in this research have been treated according to ASIH et al. (2011) criteria and with approval from the animal ethics committee of the Faculty of Biochemistry and Biological Sciences. http://www.fbcb.unl.edu.ar/pages/investigacion/comite-deetica.php.

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Attademo, A.M., Lajmanovich, R.C., Peltzer, P.M. et al. Acute Toxicity of Metaldehyde in the Invasive Rice Snail Pomacea canaliculata and Sublethal Effects on Tadpoles of a Non-target Species (Rhinella arenarum). Water Air Soil Pollut 227, 400 (2016). https://doi.org/10.1007/s11270-016-3083-9

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