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The interferon antagonistic activities of the V proteins of NDV correlated with their virulence

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Abstract

Protein V of Newcastle disease virus (NDV) serves as interferon (IFN) antagonist, and NDV stains with different pathogenicity show different abilities in inhibition IFN expression. To further reveal the relationship between viral virulence and their IFN-antagonistic activity derived from protein V, six NDV strains with three different pathotypes were used in this study and their V gene were cloned into eukaryotic expression vector. The V gene derived from different NDV strains were expressed in same level in cells after transfection according to the results from Western blotting. And these proteins showed different interferon-antagonistic activities based on interferon expression using Luciferase Reporter Assay and ELISA. The expression of IFN and viral virulence index, mean death time, have a good linear relationship indicating a good correlation between viral virulence and IFN antagonism of their V Protein.

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Data Availability

All data generated or analyzed during this study are included in this published article.

Abbreviations

IFN:

Interferon

NDV:

Newcastle disease virus

ND:

Newcastle disease

SV:

Sendai virus

SV5:

Simian virus 5

CDV:

Canine distemper virus

RPV:

Rinderpest virus

RSV:

Respiratory syncytial virus

NP:

Nucleocapsid protein

P:

Phosphoprotein

M:

Matrix protein

F:

Fusion protein

HN:

Hemagglutinin–neuraminidase protein

L:

RNA-dependent RNA polymerase

CTD:

C-terminal domain

R:

Arginine

E:

Glutamic acid

I:

Isoleucine

P:

Proline

A:

Alanine

IFN:

Interferon

STAT1:

Signal transducer and activator of transcription

MDA-5:

Melanoma differentiation-associated protein 5

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Acknowledgements

We thank Dr Shengli Chen and Huafang Hao for providing us with the plasmids harboring V genes.

Funding

This work was funded by the National Natural Science Foundation of China (No. 31672581).

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Authors and Affiliations

  1. College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, Shaanxi, People’s Republic of China

    Xinglong Wang, Ruiyi Dang & Zengqi Yang

Authors
  1. Xinglong Wang
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  2. Ruiyi Dang
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  3. Zengqi Yang
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Contributions

XW performed the experiments and made analysis of the data. RD and ZY participated preparation including discussion and editing.

Corresponding authors

Correspondence to Xinglong Wang or Zengqi Yang.

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The authors declare they have no conflict of interests.

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All the authors consent to publish.

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Edited by Zhen F. Fu.

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Wang, X., Dang, R. & Yang, Z. The interferon antagonistic activities of the V proteins of NDV correlated with their virulence. Virus Genes 55, 233–237 (2019). https://doi.org/10.1007/s11262-019-01637-3

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  • DOI: https://doi.org/10.1007/s11262-019-01637-3

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