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Immune responses elicited in mice with recombinant Lactococcus lactis expressing F4 fimbrial adhesin FaeG by oral immunization

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Abstract

Enterotoxigenic Escherichia coli (ETEC) is a major pathogenic agent causing piglet diarrhea. The major subunit and adhesin FaeG of F4+ ETEC is an important virulence factor with strong immunogenicity. To determine whether Lactococcus lactis (L. lactis) could effectively deliver FaeG to the mucosal immune system, recombinant L. lactis expressing FaeG was constructed, and immune responses in mice following oral route delivery of recombinant L. lactis were explored. The production of FaeG expressed in L. lactis was up to approximately 10% of soluble whole-cell proteins, and recombinant FaeG (rFaeG) possessed good immunoreactivity by Western blot analysis. Oral immunization with recombinant L. lactis expressing FaeG induced F4-specific mucosal and systemic immune responses in the mice. In addition, high dose recombinant L. lactis or co-administration of high dose recombinant L. lactis with CTB enhanced the immune responses. These results suggested that L. lactis expressing FaeG was a promising candidate vaccine against ETEC.

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Acknowledgments

We gratefully acknowledge the support and help from Song Gao, Guolan Bao, Cun Zhang, Minhua Liu, Bo Deng, Xin Tao, Ying Xu. Xiufang Yuan, Xiaoming Men, Fang Li, Lei Zhang.

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Authors and Affiliations

  1. State Key Lab of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, China

    Shujie Liu & Ziwei Xu

  2. Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, China

    Shujie Liu, Yongming Li, Ziwei Xu & Yicheng Wang

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  1. Shujie Liu
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  2. Yongming Li
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  3. Ziwei Xu
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  4. Yicheng Wang
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Correspondence to Ziwei Xu.

Additional information

This work was supported by Project from National Natural Science Foundation, China (30871799), and Key Science and Technology Special Projects of Zhejiang Provice, China (2005C12031, 2008C02003-2).

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Liu, S., Li, Y., Xu, Z. et al. Immune responses elicited in mice with recombinant Lactococcus lactis expressing F4 fimbrial adhesin FaeG by oral immunization. Vet Res Commun 34, 491–502 (2010). https://doi.org/10.1007/s11259-010-9418-x

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