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Combination antioxidant effect of α-tocoferol and erdosteine in ischemia–reperfusion injury in rat model

  • Urology - Original Paper
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An Erratum to this article was published on 16 October 2009

Abstract

Introduction

Renal ischemia/reperfusion (I/R) which is an important cause of renal dysfunction is inevitable in renal transplantation, surgical revascularization of the renal artery, partial nephrectomy and treatment of suprarenal aortic aneurysms.

Aim

The purpose of this study was to investigate the efficacy of α-tocopherol and erdosteine combination in the reduction in injury induced by ROS in a rat model of renal ischemia–reperfusion.

Materials and methods

Thirty-six- male Wistar albino rats weighing 200–250 g were utilized for this study. Rats were divided into six groups, and each group was consistent of six rats: (1) sham-operated (control), (2) ischemia group (3) I/R group, (4) I/R/α-tocoferol group (5) I/erdosteine group (6). I/R/α-tocoferol and erdosteine group. Biochemically tissue MDA, XO and SOD activities, light and electron microscopic findings were evaluated.

Results

The erdosteine and α-tocoferol significantly reversed the effect of protein oxidation and lipid peroxidation induced by I/R shown by the decreased levels of MDA and XO activities. Both MDA and XO levels were found to be lower in group 6 compared to single agent treatment groups, and this was significantly different. All treatment groups showed increased SOD activity, which accounts for their oxidative properties. The mean Paller score of the combination treatment group (group 6) was lower than all groups except the sham group (3.67 ± 1.2), and this finding was statistically significant (0.05). Our results showed that the antioxidant pretreatment with α-tocopherol and erdosteine combination reduced lipid peroxidation of renal cellular membranes in a model of normothermic renal ischemia–reperfusion in rats. Combination of erdosteine and α-tocopherol has a synergistic effect of protection against oxidative processes. Long-term use of α-tocopherol seems to have a greater effect on the prevention of IR injury. However, further investigations are needed for the clinical applications of our findings.

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Acknowledgments

This study was supported by the Selcuk University Research Fund.

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Authors and Affiliations

  1. Department of Urology, Selcuk University Meram Medical Faculty, Konya, Turkey

    Talat Yurdakul & Mehmet Mesut Pişkin

  2. Department of Urology, Selcuk University Selcuklu Medical Faculty, Konya, Turkey

    Haluk Kulaksizoglu

  3. Department of Pathology, Selcuk University Meram Medical Faculty, Konya, Turkey

    Mustafa Cihat Avunduk

  4. Department of Histology and Embriology, Ankara University Medical Faculty, Ankara, Turkey

    Esra Ertemli

  5. Department of Pharmacology, Selcuk University Selcuklu Medical Faculty, Konya, Turkey

    Hülagü Barişkaner

  6. Department of Biochemistry, Selcuk University Meram Medical Faculty, Konya, Turkey

    Sadik Büyükbaş & Volkan Kocabas

Authors
  1. Talat Yurdakul
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  3. Mehmet Mesut Pişkin
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Corresponding author

Correspondence to Haluk Kulaksizoglu.

Additional information

An erratum to this article can be found at http://dx.doi.org/10.1007/s11255-009-9662-6

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Yurdakul, T., Kulaksizoglu, H., Pişkin, M.M. et al. Combination antioxidant effect of α-tocoferol and erdosteine in ischemia–reperfusion injury in rat model. Int Urol Nephrol 42, 647–655 (2010). https://doi.org/10.1007/s11255-009-9641-y

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