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Antioxidant levels of common fruits, vegetables, and juices versus protective activity against in vitro ischemia/reperfusion

  • Urology - Original Paper
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Abstract

It is well known that antioxidants present in various fruits, vegetables, and juices have the potential to protect the urinary bladder from free radical damage. What is not well understood, however, is how well antioxidant activities detected by chemical methods such as the CUPRAC assay for total antioxidant activity (TAA) predict the level of physiological protection available. It is hypothesized that the level of antioxidant reactivity found by the CUPRAC assay will positively correlate with increased protection in a model of in vitro ischemia/reperfusion. To test this hypothesis, the CUPRAC assay was utilized to determine the antioxidant reactivity of a series of fruits, vegetables, and juices, and the results were compared to the protective ability of selected juices in an established in vitro rabbit bladder model of ischemia/reperfusion. The results of the CUPRAC test showed that cranberry juice had the highest level of antioxidant reactivity, blueberry juice had an intermediate activity, and orange juice had the lowest. It was determined, however, that contrary to the hypothesis, the orange juice was significantly more potent in protecting the bladder against ischemia/reperfusion damage than either blueberry or cranberry juice. Thus, it is concluded that chemical tests for TAA do not necessarily correlate with their physiological activity.

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Acknowledgments

This work was supported in part by the Office of Research and Development of the Department of Veterans Affairs and by the Capital Region Medical Research Foundation.

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Authors and Affiliations

  1. Department of Pharmaceutical Sciences, Albany College of Pharmacy and Health Sciences, Stratton VA Medical Center and Albany Medical College, 106 New Scotland Ave, Albany, NY, 12208, USA

    Holly Bean, Catherine Schuler, Robert E. Leggett & Robert M. Levin

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  1. Holly Bean
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  2. Catherine Schuler
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  3. Robert E. Leggett
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  4. Robert M. Levin
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Correspondence to Robert M. Levin.

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Bean, H., Schuler, C., Leggett, R.E. et al. Antioxidant levels of common fruits, vegetables, and juices versus protective activity against in vitro ischemia/reperfusion. Int Urol Nephrol 42, 409–415 (2010). https://doi.org/10.1007/s11255-009-9639-5

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  • DOI: https://doi.org/10.1007/s11255-009-9639-5

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