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DNA-binding and photocleavage studies of metallofluorescein–porphyrin complexes of zinc(II) and copper(II)

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Abstract

The DNA-binding behaviors of the fluorescein–porphyrinatozinc(II) complex Zn(Fl-PPTPP) (Fl-PPTPP = 5-(4-fluoresceinpropyloxy)phenyl-10,15,20-triphenylporphyrin) and fluorescein–porphyrinatocopper(II) complex Cu(Fl-PPTPP) with calf thymus DNA (CT-DNA) were investigated by UV–Vis absorption titrations, fluorescence spectra, viscosity measurements, thermal denaturation and circular dichroism. The results suggest that both complexes interact with CT-DNA by intercalation. In addition, their photocleavage reactions with pBR322 supercoiled plasmid DNA were investigated. Both complexes exhibit significant DNA cleavage activity, and singlet oxygen may play an important role in these reactions.

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Acknowledgments

We gratefully acknowledge the financial support for this work by Guangdong Pharmaceutical University (No. 43540119) and the National Natural Science Foundation of P. R. China (No. 81102753 &21101033).

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Authors and Affiliations

  1. School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, 510006, People’s Republic of China

    Jiazheng Lu, Weijian Pan, Rongwei He, Shuqiang Jin, Xiangwen Liao & Haiwei Guo

  2. The Peoples’ Hospital of Shiling Huadu District, Guangzhou, 510850, Guangdong, People’s Republic of China

    Bin Wu

  3. School of Medicine Chemistry and Chemical Engineering, Guangdong Pharmaceutical University, Guangzhou, 510006, People’s Republic of China

    Ping Zhao

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  1. Jiazheng Lu
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  2. Weijian Pan
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  5. Xiangwen Liao
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Correspondence to Jiazheng Lu or Ping Zhao.

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Lu, J., Pan, W., He, R. et al. DNA-binding and photocleavage studies of metallofluorescein–porphyrin complexes of zinc(II) and copper(II). Transition Met Chem 37, 497–503 (2012). https://doi.org/10.1007/s11243-012-9615-2

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  • DOI: https://doi.org/10.1007/s11243-012-9615-2

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