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In vitro propagation of Farsetia macrantha Blatt. & Hallb.: an endemic and threatened plant of Indian Thar Desert

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Abstract

Farsetia macrantha Blatt. & Hallb. is an endemic and critically endangered plant species of Indian Thar desert. Being an endemic and threatened plant species, there is need to develop an efficient in vitro regeneration protocol for its conservation. Since, there is no published report on in vitro regeneration of F. macrantha, therefore, present study was designed to develop an efficient micropropagation method of F. macrantha along with foliar micromorphological studies. Cotyledonary node taken from 15 to 20 days old seedlings was used as an explant for in vitro regeneration. MS medium supplemented with additives ascorbic acid (50 mg l−1), citric acid (25 mg l−1), l-arginine (25 mg l−1) and adenine sulphate (25 mg l−1) and 0.5 mg l−1 BA was best for shoot bud induction. Different types, concentrations and combinations of cytokinins (BA, Kin and mT) and auxin (IAA) tested for shoot multiplication, maximum number of shoots (25.10 ± 0.69) regenerated on MS medium containing additives and combination of two cytokinins 0.5 mg l−1 BA + 0.25 mg l−1 Kin. Microshoots were rooted successfully with highest rooting response (75.59%) on ½ MS medium containing 2.0 mg l−1 IBA. In vitro rooted plants were hardened with ~ 75% survival rate. Comparative foliar micromorphological studies conducted among in vitro grown and hardened plants suggest the developmental changes of micropropagated plants during acclimatization. The present in vitro regeneration protocol could be used for large-scale propagation and conservation of F. macrantha.

Key message

Farsetia macrantha is an endemic and critically endangered plant species of Indian Thar desert and presently it is restricted only to the Barmer district of Rajasthan, India. In the present study, an in vitro regeneration method has been developed first time for its conservation.

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Acknowledgements

Authors (DC and VK) gratefully acknowledges to the University Grant Commission (UGC), New Delhi and National Medicinal Plant Board (NMPB) (Pr. No. R&D/RAJ-01/2016-17) for financial support.

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Authors and Affiliations

  1. Biotechnology Unit, Department of Botany (UGC-Centre of Advanced Study), Jai Narain Vyas University, New Campus, Jodhpur, Rajasthan, 342001, India

    Dheeraj Choudhary, N. S. Shekhawat & Vinod Kataria

  2. Department of Environmental Science, Indira Gandhi National Tribal University, Amarkantak, Madhya Pradesh, 484887, India

    Manoj K. Rai

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  1. Dheeraj Choudhary
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  2. Manoj K. Rai
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  3. N. S. Shekhawat
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  4. Vinod Kataria
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Contributions

DC performed the experiments and analyzed the data. MKR gave input in experimental design and data analysis. DC and MKR wrote manuscript. NSS and VK supervised the experiments and edited final version of manuscript. All authors read and approved the final version of manuscript.

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Correspondence to Vinod Kataria.

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Communicated by Amita Bhattacharya.

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Choudhary, D., Rai, M.K., Shekhawat, N.S. et al. In vitro propagation of Farsetia macrantha Blatt. & Hallb.: an endemic and threatened plant of Indian Thar Desert. Plant Cell Tiss Organ Cult 142, 519–526 (2020). https://doi.org/10.1007/s11240-020-01876-5

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