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Pi-starvation is mitigated in Medicago truncatula plants with upregulated auxin transport through auxin–strigolactone interaction

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Abstract

One of the most important micronutrients required for normal development of plants is phosphorus. In terms of phosphorus starvation, plants have developed plasticity for overcoming stress, expressed in the formation of shortened and thickened cluster roots. Both phytohormones auxin and strigolactone participate in the plant response associated with the phosphate signaling. The present study revealed the interaction and correlation between auxin and strigolactones in Medicago truncatula plants with modified auxin transport under extreme conditions of phosphate deficiency and excess. In general, drastic changes in the root architecture were observed in both overexpression and RNAi-lines. In terms of phosphate deprivation the number of lateral roots has gained and the length of main root was reduced. Relative transcript level of MtLAX3 gene and the two strigolactone genes MtMAX2 and MtMAX3 was upregulated in OE/RNAi-lines and wild type plants in terms of phosphorus starvation compare to same lines in normal condition.

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Acknowledgements

This study was supported by a Grant from National Science Fund at the Ministry of Education and Science of the Republic of Bulgaria, Project DM06/2 “Hormonal crosstalk between auxin and strigolactones in Medicago truncatula plants with modified auxin transport”. The authors are grateful for technical help of Kety Krastanova.

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  1. AgroBioInstitute, Blvd. Dragan Tzankov 8, 1164, Sofia, Bulgaria

    Miglena Revalska & Anelia Iantcheva

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  1. Miglena Revalska
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  2. Anelia Iantcheva
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Contributions

MR designed the research and performed the experiments. MR and AI analysed the data and results. MR drafted the manuscript. AI revised the manuscript. All authors read and approved the manuscript.

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Correspondence to Anelia Iantcheva.

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Additional information

Communicated by Sergio J. Ochatt.

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Supplementary material 1 (DOCX 15 KB)

Supplemental Figure 1

. Relative expression level of MtLAX3 in T0 and T1 M. truncatula MtLAX3-OE and MtLAX3-RNAi compared to WT plants (JPG 2522 KB)

Supplemental Figure 2

. Descriptive statistics of Two-Way ANOVA and “Interaction plot” between the two factors “treatment” and “genotype” for main root length (MRL) (JPG 7443 KB)

Supplemental Figure 3

. Descriptive statistics of Two-Way ANOVA and “Interaction plot” between the two factors “treatment” and “genotype” for lateral root number (LRN) (JPG 7243 KB)

Supplemental Figure 4

. Descriptive statistics of Two-Way ANOVA and “Interaction plot” between the two factors “treatment” and “genotype” for relative expression level of MtLAX3 (JPG 5269 KB)

Supplemental Figure 5

. Descriptive statistics of Two-Way ANOVA and “Interaction plot” between the two factors “treatment” and “genotype” for relative expression level of MtMAX2 (JPG 5315 KB)

Supplemental Figure 6

. Descriptive statistics of Two-Way ANOVA and “Interaction plot” between the two factors “treatment” and “genotype” for relative expression level of MtMAX3 (JPG 5587 KB)

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Revalska, M., Iantcheva, A. Pi-starvation is mitigated in Medicago truncatula plants with upregulated auxin transport through auxin–strigolactone interaction. Plant Cell Tiss Organ Cult 133, 405–415 (2018). https://doi.org/10.1007/s11240-018-1393-x

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