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Potato dehydrins present high intrinsic disorder and are differentially expressed under ABA and abiotic stresses

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Abstract

Dehydrins (DHNs) correspond to late embryogenesis abundant proteins (LEA) of group 2, they are known as glycin rich proteins. Despite their expression during the late seed maturation stages, they are also involved in plant response to a number of abiotic stresses such as drought, salinity and cold. In the present study, we identified five full-length cDNAs encoding dehydrins (designated StDHN2a, StDHN1, TAS14, StDHN25 and StLEA27) isolated from potato. These dehydrins were composed of serine amino acids called S domain and lysine-rich segment corresponding to a K domain. Three DHNs (StDHN1, TAS14 and StLEA27) contained Y segments. In silico analysis showed that these StDHN sequences share high homology with other Solanum dehydrin proteins species. The analysis of gene expression using quantitative RT-PCR showed that they were upregulated by dehydration and salinity. Moreover, the search for putative regulatory element in the promoter sequence of dehydrin genes was investigated.

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Acknowledgments

This work was supported by the Tunisian Ministry of High Education and Scientific Research. The authors are grateful to Dr. Anne-Lise Haenni from the Institute Jacques Monod (France) for reading and improving the English.

Author contributions

SC, MC, and RJ carried out the experimental work. MNS and RGB participated in the data analysis and contributed in writing and revising the manuscript.

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Authors and Affiliations

  1. Laboratoire d’Amélioration des Plantes et Valorisation des Agroressources, Ecole Nationale d’Ingénieurs de Sfax, Route Soukra Km 4, B.P 1173, 3038, Sfax, Tunisia

    Safa Charfeddine, Mariam Charfeddine & Radhia Gargouri Bouzid

  2. Centre de Biotechnologie de Sfax, Route Sidi Mansour Km 4, B.P 1177, 3018, Sfax, Tunisia

    Mohammed Najib Saïdi

  3. Laboratoire des Biotechnologies végétales appliquées à l’amélioration des cultures, Faculté des sciences de Sfax, Route Soukra Km 4, B.P 1171, 3038, Sfax, Tunisia

    Rania Jbir

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  1. Safa Charfeddine
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Correspondence to Safa Charfeddine.

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Charfeddine, S., Charfeddine, M., Saïdi, M.N. et al. Potato dehydrins present high intrinsic disorder and are differentially expressed under ABA and abiotic stresses. Plant Cell Tiss Organ Cult 128, 423–435 (2017). https://doi.org/10.1007/s11240-016-1120-4

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