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Cotton TCTP1 gene encoding a translationally controlled tumor protein participates in plant response and tolerance to aphids

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Abstract

Cotton aphid (Aphis gossypii Glover) is one of the most important economic pests in the world. Long-term unreasonable usage of insecticides has made cotton aphid developing insecticide-resistance, which frequently leads to serious occurrences of cotton aphid in many regions. It is regarded effective and environmentally friendly to control aphids through utilizing plant resistance. In this study, a translationally controlled tumor protein gene, GhTCTP1, was isolated in cotton. It belongs to TCTP subfamily and encodes a protein of 168 amino acids. GhTCTP1 expression was suppressed in cotton plants under cotton aphid attack, but its expression level was up-regulated in the wounded cotton leaves. The choice test and no-choice test demonstrated that overexpression of GhTCTP1 in Arabidopsis enhanced plant resistance to green peach aphid (Myzus persicae). Quantitative RT-PCR analysis revealed that the defense response genes related to salicylic acid signaling pathway were activated in the GhTCTP1 overexpressing transgenic plants. Content of total amino acids was decreased, and phenylalanine ammonialyase activity was altered in leaves of the transgenic Arabidopsis plants, compared with those in wild type. Furthermore, the callose amount in transgenic Arabidopsis leaves was more than that of wild type. These data suggested that GhTCTP1 might be involved in regulation of plant tolerance to aphids, and can be potentially applied in improving aphid-resistance of crops by genetic manipulation.

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Abbreviations

TCTP:

Translationally controlled tumour protein

GFP:

Green fluorescent protein

GPA:

Green peach aphid

POD:

Peroxidase

SOD:

Superoxide dismutase

PAL:

Phenylalanine ammonialyase

RT-PCR:

Reverse-transcriptase polymerase chain reaction

SA:

Salicylic acid

JA:

Jasmonic acid

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Acknowledgments

This work was supported by the projects from the Ministry of Agriculture of China (Grant No. 2014ZX08009-027B, 2014ZX08009003-004), Hubei Key Laboratory of Genetic Regulation and Integrative Biology (Grant No. 201008) and Scientific Research Foundation of Hubei Province (Grant No. 2013CFA119).

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  1. Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan, 430079, China

    Jian-Min Zhang, Liu-Feng Ma, Wen Li, Jie Zhang, Deng-Di Li & Xue-Bao Li

  2. College of Agriculture, Yangtze University, Jingzhou, 434023, China

    Jian-Min Zhang

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  1. Jian-Min Zhang
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  2. Liu-Feng Ma
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Correspondence to Xue-Bao Li.

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Zhang, JM., Ma, LF., Li, W. et al. Cotton TCTP1 gene encoding a translationally controlled tumor protein participates in plant response and tolerance to aphids. Plant Cell Tiss Organ Cult 117, 145–156 (2014). https://doi.org/10.1007/s11240-014-0426-3

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