Skip to main content

Advertisement

SpringerLink
Log in

Cytokinin and explant types influence in vitro plant regeneration of Leopard Orchid (Ansellia africana Lindl.)

  • Original Paper
  • Published:
Plant Cell, Tissue and Organ Culture (PCTOC) Aims and scope Submit manuscript

Abstract

The effects of explant and cytokinin types on in vitro plant regeneration of Ansellia africana were investigated. The exogenous addition of cytokinins is not required for the proliferation of new protocorms from Trimmed protocorm cluster (TPC) explants. To the contrary, nodal and shoot-tip explants produced a single shoot in response to the addition of cytokinins. Overall plant growth in terms of shoot length, leaf number, frequency of root organogenesis, root length, and fresh weight/plant were significantly higher in media containing meta-Topolin Riboside (mTR) in both nodal and shoot-tip explants. Thidiazuron (TDZ) and 6-benzyladenine (BA) induced stunted and hypertrophied shoots at their highest level (15 μM). In addition root differentiation and root growth were significantly lower on P668 media with TDZ and BA. Zeatin was capable of inducing a significantly higher root organogenesis frequency and root length in TPC explants as compared to other cytokinins. However, TPC explants produced a significantly greater number of longer shoots (>3 cm) on P668 media with mTR. Hyperhydric shoots were produced from TPC explants. The occurrence of hyperhydricity is discussed with respect to the culture vessel used in this study.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

Abbreviations

BA:

6-benzyladenine

DMRT:

Duncan’s multiple range test

mTR:

meta-topolin riboside

P668:

Phytotechnology medium

TDZ:

Thidiazuron

TPC:

Trimmed protocorm cluster

References

  • Amoo SO, Finnie JF, Van Staden J (2011) The role of meta-topolins in alleviating micropropagation problems. Plant Growth Regul 63:197–206

    Article  CAS  Google Scholar 

  • Bodhipadma K, Noichinda S, Padyencheun W, Khunthacharoen T, Chikhunthod U, Leung DWM (2010) Influence of preculture treatment and types of explants on shoot growth and in vitro flowering of feathered amaranth (Celosia argentea var. plumose). Plant Cell Tiss Organ Cult 105:465–469

    Article  Google Scholar 

  • Chen JT, Chang WC (2006) Direct somatic embryogenesis and plant regeneration from leaf explants of Phalaenopsis amabilis. Biol Plant 50:169–173

    Article  Google Scholar 

  • Chen YC, Chang C, Chang WC (2000) A reliable protocol for plant regeneration from callus culture of Phalaenopsis. In vitro Cell Dev Biol Plant 36:420–423

    Article  CAS  Google Scholar 

  • Chugh S, Guha S, Rao U (2009) Micropropagation of orchids: a review on the potential of different explants. Sci Hortic 122:507–520

    Article  CAS  Google Scholar 

  • Coste A, Vlase L, Halmagyi A, Deliu C, Coldea G (2011) Effects of plant growth regulators and elicitors on production of secondary metabolites in shoot cultures of Hypericum hirsutum and Hypericum maculatum. Plant Cell Tiss Organ Cult. doi: 10.1007/s11240-011-9919-5

  • Duncan G (2005) Bartholina burmanniana: Orchidaceae. Curtis’s Bot Mag 22:25–31

    Article  Google Scholar 

  • Ernst R (1994) Effect of thidiazuron on in vitro propagation of Phalaenopsis and Doritaenopsis (Orchidaceae). Plant Cell Tiss Org Cult 39:273–275

    Article  CAS  Google Scholar 

  • George EF (1993) Plant propagation by tissue culture, Part I, The technology. Exegetics Ltd., Basingstoke, UK

    Google Scholar 

  • Golding JS (2002) Southern African plant red data lists. Southern African botanical diversity network report No. 14. SABONET, Pretoria, South Africa

  • Gómez MP, Segura J (1995) Axillary shoot proliferation in cultures of explants from mature Juniperus oxycedrus trees. Tree Physiol 15:625–628

    PubMed  Google Scholar 

  • Grodzinski B, Boesel I, Horton K (1981) Effect of light and carbon dioxide on release of ethylene from leaves of Xanthium strumarium. Plant Physiol 67:272–273

    Google Scholar 

  • Hussey G (1976) In vitro release of axillary shoots from apical dominance in monocotyledonous plantlets. Ann Bot 40:1323–1325

    Google Scholar 

  • Ivanova M, Van Staden J (2010) Natural ventilation effectively reduces hyperhydricity in shoot cultures of Aloe polyphylla Schönland ex Pillans. Plant Growth Regul 60:143–150

    Article  CAS  Google Scholar 

  • Jones OP, Hopgood ME, O’Farrel D (1977) Propagation in vitro of M.26 apple rootstocks. J Hortic Sci 52:235–238

    Google Scholar 

  • Kukulczanka K, Wojciechowska U (1983) Propagation of two Dendrobium species by in vitro culture. Acta Hortic 131:105–110

    Google Scholar 

  • Lai C-C, Lin H-M, Nalawade SM, Fang W, Tsay H-S (2005) Hyperhydricity in shoot cultures of Scrophularia yoshimurae can be effectively reduced by ventilation of culture vessels. J Plant Physiol 162:355–361

    Article  PubMed  CAS  Google Scholar 

  • Lane WD (1978) Regeneration of apple plants from shoot meristem tips. Plant Sci Lett 13:281–285

    Article  CAS  Google Scholar 

  • Magyar-Tábori K, Dobránski J, Teixeira da silva JA, Bulley SM, Hudák I (2010) The role of cytokinins in shoot organogenesis in apple. Plant Cell Tiss Organ Cult 101:251–267

    Article  Google Scholar 

  • Mostafa SE, Karam NS, Shibli RA, Alali FQ (2010) Micropropagation and production of arbutin in oriental strawberry tree (Arbutus andrachne L.). Plant Cell Tiss Organ Cult 103:111–121

    Article  Google Scholar 

  • Murdad R, Hwa KS, Seng CK, Latip MA, Aziz ZA, Ripin R (2006) High frequency multiplication of Phalaenopsis gigantea using trimmed bases protocorms technique. Sci Hortic 111:73–79

    Article  CAS  Google Scholar 

  • Parker CW, Letham DS, Gollnow BI, Summons RE, Duke CC, Macleod JK (1978) Regulators of cell division in plant tissues XXV. Metabolism of zeatin by lupin seedlings. Planta 142:239–251

    Article  CAS  Google Scholar 

  • Sriskandarajah S, Mullins MG (1981) Micropropagation of Granny Smith apple: factors affecting root formation in vitro. J Hortic Sci 56:71–76

    Google Scholar 

  • Strnad M (1997) The aromatic cytokinins. Physiol Plant 101:674–688

    Article  CAS  Google Scholar 

  • Valero-Aracama C, Kane ME, Wilson SB, Philman NL (2010) Substitution of benzyladenine with meta-topolin during shoot multiplication increases acclimatization of difficult- and easy-to acclimatize sea oats (Uniola paniculata L.) genotypes. Plant Growth Regul 60:43–49

    Article  CAS  Google Scholar 

  • Vasudevan R, Van Staden J (2010) In vitro asymbiotic seed germination and seedling growth of Ansellia africana Lindl. Sci Hortic 123:496–504

    Article  CAS  Google Scholar 

  • Wang Y, Luo JP, Wei ZJ, Zhang JC (2009) Molecular cloning and expression analysis of a cytokinin oxidase (DhCKX) gene in Dendrobium huoshanense. Mol Biol Rep 36:1331–1338

    Article  PubMed  CAS  Google Scholar 

  • Webster CA, Jones OP (1991) Micropropagation of some cold-hardy dwarfing rootstocks for apple. J Hortic Sci 66:1–6

    CAS  Google Scholar 

  • Werbrouck SPO, Van der Jeugt B, Dewitte W, Prinsen E, Van Onckelen HA, Debergh PC (1995) The metabolism of benzyladenine in S. floribundum Schott ‘Petite’ in relation to acclimatisation problems. Plant Cell Rep 14:662–665

    Article  CAS  Google Scholar 

  • Werbrouck SPO, Strnad M, Van Onckelen H, Debergh P (1996) Meta-topolin, an alternative to benzyladenine in tissue culture? Physiol Plant 98:291–297

    Article  CAS  Google Scholar 

  • Werner EM, Boe AA (1980) In vitro propagation of Malling 7 apple rootstock. Hortic Sci 15:509–510

    Google Scholar 

Download references

Acknowledgments

The authors thank the National Research Foundation (NRF), Pretoria for financial support.

Author information

Authors and Affiliations

  1. Research Centre for Plant Growth and Development, School of Biological and Conservation Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, Scottsville, 3209, South Africa

    Rajeswari Vasudevan & Johannes Van Staden

Authors
  1. Rajeswari Vasudevan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Johannes Van Staden
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Johannes Van Staden.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Vasudevan, R., Van Staden, J. Cytokinin and explant types influence in vitro plant regeneration of Leopard Orchid (Ansellia africana Lindl.). Plant Cell Tiss Organ Cult 107, 123–129 (2011). https://doi.org/10.1007/s11240-011-9964-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11240-011-9964-0

Keywords

Search

Navigation