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The role of abscisic acid in plant tissue culture: a review of recent progress

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Abstract

Abscisic acid (ABA) plays a significant role in the regulation of many physiological processes of plants. It is often used in tissue culture systems to promote somatic embryogenesis and enhance somatic embryo quality by increasing desiccation tolerance and preventing precocious germination. ABA is also employed to induce somatic embryos to enter a quiescent state in plant tissue culture systems and during synthetic seed research. Application of exogenous ABA improves in vitro conservation and the adaptive response of plant cell and tissues to various environmental stresses. ABA can act as anti-transpirant during the acclimatization of tissue culture-raised plantlets and reduces relative water loss of leaves during the ex vitro transfer of plantlets even when non-functional stomata are present. This review focuses on the possible roles of ABA in plant tissue culture and recent developments in this area.

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Acknowledgments

The authors (M.K. Rai and Harish) wish to acknowledge to University Grants Commission (UGC), New Delhi for the award of the Dr. D.S. Kothari Post Doctoral Fellowship. We are also grateful to the Department of Biotechnology (DBT), Department of Science and Technology (DST), Council of Scientific and Industrial Research (CSIR) and University Grants Commission (UGC), New Delhi for financial support. Valuable suggestions by the anonymous reviewers for improving the manuscript are also very much appreciated.

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Authors and Affiliations

  1. Department of Botany, Biotechnology Centre, Jai Narain Vyas University, Jodhpur, Rajasthan, 342033, India

    Manoj K. Rai, N. S. Shekhawat,  Harish, Amit K. Gupta, M. Phulwaria, Kheta Ram & U. Jaiswal

  2. Department of Botany, Mahila Mahavidyalaya, Banaras Hindu University, Varanasi, Uttar Pradesh, 221005, India

    U. Jaiswal

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  1. Manoj K. Rai
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  2. N. S. Shekhawat
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  3. Harish
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  6. Kheta Ram
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  7. U. Jaiswal
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Correspondence to Manoj K. Rai.

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The work is dedicated to the first author’s late supervisor Prof. V.S. Jaiswal (1945–2007).

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Rai, M.K., Shekhawat, N.S., Harish et al. The role of abscisic acid in plant tissue culture: a review of recent progress. Plant Cell Tiss Organ Cult 106, 179–190 (2011). https://doi.org/10.1007/s11240-011-9923-9

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