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In vitro haploid and dihaploid production via unfertilized ovule culture

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Abstract

Haploids and doubled haploids are very important in plant breeding, enabling the time needed to produce homozygous lines to be shortened compared with conventional breeding. In the present review, emphasis is given to haploid induction through unfertilized ovule/ovary culture. Attention is given to induction of haploid plants from female gametophyte culture through analysis of factors in the processes of gynogenesis, including genotype selection, stage of ovule development, pretreatment, and culture media containing nutritional components and phytohormones. The gynogenetic approach may be of great value in discovering novel genetic recombinations. Application of double haploids in genetics and plant breeding is also highlighted. This review also identifies some existing knowledge gaps where work may increase the efficiency of this process in different plant species.

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Correspondence to Jin-Feng Chen.

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Jin-Feng Chen and Li Cui contributed equally to this paper.

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Chen, JF., Cui, L., Malik, A.A. et al. In vitro haploid and dihaploid production via unfertilized ovule culture. Plant Cell Tiss Organ Cult 104, 311–319 (2011). https://doi.org/10.1007/s11240-010-9874-6

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