Abstract
The effect of salinity on the non-enzymic and enzymic antioxidant activity, shoot proliferation and nutrient accumulation was studied in in vitro cultures of the rootstock CAB-6P (Prunus cerasus L.). Three concentrations (0, 30 and 60 mM) of NaCl or CaCl2 were added to a modified MS medium. Between the two salt treatments used, only the explants treated with CaCl2 presented significant decrease in growth parameters. The concentrations of Na+ and Cl− in the explants treated with NaCl were increased, as NaCl in the culture medium increased. Furthermore, in the explants treated with CaCl2 the concentrations of Ca2+ and Cl− were increased while that of K+ decreased, as CaCl2 concentration increased. The activity of peroxidase in leaves as well as the number of its anionic isoforms was increased under 30 mM CaCl2 as well as 60 mM NaCl or CaCl2. On the contrary, increasing salinity, from 0 to 60 mM CaCl2, resulted in a reduction of the catalase activity in leaves followed by disappearance of the only one catalase isoform that was detected in leaves (60 mM CaCl2). In the stems of the explants treated with NaCl the peroxidase activity was reduced. In the stems and leaves of the explants grown in saline substrate the non-enzymic antioxidant activity was significantly increased. The results suggest that the stems and leaves of CAB-6P explants presented variable antioxidant responses that were depended on the salt form used. The contribution of enzymic and non-enzymic protection mechanisms to the adaptation of CAB-6P explants under salinity stress is discussed.
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Abbreviations
- BA:
-
6-benzylaminopurine
- CAT:
-
Catalase
- DMAB:
-
3-dimethylamino benzoic acid
- FRAP:
-
Ferric reducing antioxidant power
- IBA:
-
Indolebutyric acid
- MBTH:
-
3-methyl-2-benzothiazoline hydrazone hydrochloride monohydrate
- MS:
-
Murashige Skoog
- POD:
-
Peroxidase
- PVPP:
-
Polyvinylpolypyrrolidone
- ROS:
-
Reactive oxygen species
- SOD:
-
Superoxide dismutase
- TPTZ:
-
2,4,6- tripyridyl-s-triazine
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Chatzissavvidis, C., Veneti, G., Papadakis, I. et al. Effect of NaCl and CaCl2 on the antioxidant mechanism of leaves and stems of the rootstock CAB-6P (Prunus cerasus L.) under in vitro conditions. Plant Cell Tiss Organ Cult 95, 37–45 (2008). https://doi.org/10.1007/s11240-008-9411-z
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DOI: https://doi.org/10.1007/s11240-008-9411-z