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Zeatin-induced one-step in vitro cloning affects the vegetative growth of cranberry (Vaccinium macrocarpon Ait.) micropropagules over stem cuttings

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Abstract

Shoot proliferation and rooting of three cranberry (Vaccinium macrocarpon Ait.) cultivars Bergman, Pilgrim, and Stevens were obtained in vitro on a modified nutrient medium containing zeatin following a one-step procedure. Bergman and Stevens differed in terms of shoot height, leaf number per shoot, rooting frequency, root number per explant, and root length; this was manifested with various concentrations of zeatin. Shoots proliferated and roots developed best when nodal segments were cultured in the medium supplemented with very low concentration of zeatin (2–4 μM). Such zeatin-induced tissue culture (TC) shoots of Bergman, Pilgrim, and Stevens were rooted ex vitro and compared with those propagated by conventional softwood cuttings (SC) for growth and morphology over four growth seasons. Significant interactions for leaf number per upright were observed among the treatments. The cultivars differed in terms of runner number per plant, upright length, number of leaves per upright, and shoot vigor. The propagation method had an effect on morphology of cranberry plants. The TC plants produced more runners and uprights with more leaves per upright than the conventional cuttings. This increase in vegetative growth of in vitro-derived plants over stem cuttings varied among genotypes. In vitro culture on zeatin-containing nutrient medium apparently induces the juvenile branching characteristics that favored enhanced vegetative growth with more shoots and leaf production.

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Abbreviations

2iP:

N6-[2-Isopentenyl]adenine

PPF:

Photosynthetic photon flux

SC:

Stem cutting

TC:

Tissue culture

Zeatin:

[6-(4-Hydroxy-3methylbut-trans-2-enylamino) purine]

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Acknowledgments

Atlantic Cool Climate Crop Research Centre contribution no. 196. The author gratefully acknowledges the excellent technical help from Sarah Devine, Glen Chubbs, and from Darryl Martin.

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  1. Atlantic Cool Climate Crop Research Centre, Agriculture and Agri-Food Canada, 308 Brookfield Road, P.O. Box 39088, St. John’s, NL, Canada, A1E 5Y7

    Samir C. Debnath

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Correspondence to Samir C. Debnath.

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Debnath, S.C. Zeatin-induced one-step in vitro cloning affects the vegetative growth of cranberry (Vaccinium macrocarpon Ait.) micropropagules over stem cuttings. Plant Cell Tiss Organ Cult 93, 231–240 (2008). https://doi.org/10.1007/s11240-008-9366-0

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