Abstract
Guadua angustifolia Kunth was successfully propagated in vitro from axillary buds. Culture initiation, bud sprouting, shoot and plant multiplication, rooting and acclimatization, were evaluated. Best results were obtained using explants from greenhouse-cultivated plants, following a disinfection procedure that comprised the sequential use of an alkaline detergent, a mixture of the fungicide Benomyl and the bactericide Agri-mycin, followed by immersion in sodium hypochlorite (1.5% w/v) for 10 min, and culturing on Murashige and Skoog medium containing 2 ml l−1 of Plant Preservative Mixture®. Highest bud sprouting in original explants was observed when 3 mg l−1 N6-benzylaminopurine (BAP) was incorporated into the culture medium. Production of lateral shoots in in vitro growing plants increased with BAP concentration in culture medium, up to 5 mg l−1, the highest concentration assessed. After six subcultures, clumps of 8–12 axes were obtained, and their division in groups of 3–5 axes allowed multiplication of the plants. Rooting occurred in vitro spontaneously in 100% of the explants that produced lateral shoots. Successful acclimatization of well-rooted clumps of 5–6 axes was achieved in the greenhouse under mist watering in a mixture of soil, sand and rice hulls (1:1:1).
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Abbreviations
- BAP:
-
N6-benzylaminopurine
- Ca(OCl)2 :
-
calcium hypochlorite
- PGR:
-
plant growth regulator
- PPM:
-
Plant Preservative Mixture
- NaOCl:
-
sodium hypochlorite
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Acknowledgements
We thank the management of the Estación Experimental Los Diamantes, Ministerio de Agricultura y Ganadería, Guápiles, Costa Rica for providing the plant material. Assistance of A.C. Calvo-Retana during the initial steps of this work is gratefully acknowledged. This investigation was financed with funds from the European Commission project EU INCO ICA4-CT-2001-10091. Authors also wish to thank anonymous reviewers and managing editor for helpful comments.
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Jiménez, V., Castillo, J., Tavares, E. et al. In vitro propagation of the neotropical giant bamboo, Guadua angustifolia Kunth, through axillary shoot proliferation. Plant Cell Tiss Organ Cult 86, 389–395 (2006). https://doi.org/10.1007/s11240-006-9120-4
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DOI: https://doi.org/10.1007/s11240-006-9120-4