Abstract
The species Solanum surattense Burm.f. has importance in ayurvedic medicine and also as vegetable. Streptomycin-resistant plantlets were induced showing chloroplast encoded mutants in S. surattense from mutagenised (ethyl methane sulphonate and gamma-rays) cotyledon explants. Chloroplast encoded – streptomycin resistant – shoots were developed from green (unbleached) sectors of the cotyledons. The streptomycin-resistant plants were similar to parental plants in morphology and ploidy level (2n=2x=24). Reciprocal crosses between streptomycin-resistant and the original streptomycin sensitive plants have shown the non-Mendelian transmission under the control of chloroplast – DNA. These antibiotic resistant plants are useful in designing biochemical selection schemes aimed at somatic hybrid/cybrid recovery in S. surattense.
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Abbreviations
- BAP:
-
6-benzylamino purine
- cp-DNA:
-
chloroplast DNA
- EMS:
-
ethyl methane sulphonate
- IAA:
-
indole-3-acetic acid
- MS:
-
Murashige and Skoog
- NEU:
-
nitroso ethyl urea
- NMU:
-
nitroso methyl urea
- RM:
-
regeneration medium
- SR:
-
streptomycin resistant
- SS:
-
streptomycin sensitive
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Swamy, N.R., Ugandhar, T., Praveen, M. et al. Induction of streptomycin-resistant plantlets in Solanum surattense through in vitro mutagenesis. Plant Cell Tiss Organ Cult 80, 201–207 (2005). https://doi.org/10.1007/s11240-004-0737-x
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DOI: https://doi.org/10.1007/s11240-004-0737-x