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Induction of streptomycin-resistant plantlets in Solanum surattense through in vitro mutagenesis

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Abstract

The species Solanum surattense Burm.f. has importance in ayurvedic medicine and also as vegetable. Streptomycin-resistant plantlets were induced showing chloroplast encoded mutants in S. surattense from mutagenised (ethyl methane sulphonate and gamma-rays) cotyledon explants. Chloroplast encoded – streptomycin resistant – shoots were developed from green (unbleached) sectors of the cotyledons. The streptomycin-resistant plants were similar to parental plants in morphology and ploidy level (2n=2x=24). Reciprocal crosses between streptomycin-resistant and the original streptomycin sensitive plants have shown the non-Mendelian transmission under the control of chloroplast – DNA. These antibiotic resistant plants are useful in designing biochemical selection schemes aimed at somatic hybrid/cybrid recovery in S. surattense.

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Abbreviations

BAP:

6-benzylamino purine

cp-DNA:

chloroplast DNA

EMS:

ethyl methane sulphonate

IAA:

indole-3-acetic acid

MS:

Murashige and Skoog

NEU:

nitroso ethyl urea

NMU:

nitroso methyl urea

RM:

regeneration medium

SR:

streptomycin resistant

SS:

streptomycin sensitive

References

  • MH Chen WP Yang CC Chen YK Lao TY Lin (1993) ArticleTitlePoint mutation in the chloroplast sp 12 genes from Nicotiana plumbaginifolia confers, Streptomycin resistance Plant Mol. Biol. 23 179–183

    Google Scholar 

  • BD Davis PC Tai BJ Wallace (1974) Complex interactions of antibiotics with the ribosome M Nomura A Tissueres P Lengeys (Eds) Ribosomes Cold Springer Harbour Laboratory New York 771–791

    Google Scholar 

  • D Edwards (1980) Antimicrobiol Drug Action MacMillion Pvt. Co. London 193–216

    Google Scholar 

  • T Etzold CC Fritz J Schell PH Schreir (1987) ArticleTitleA point mutation in the chloroplast 16s of a RNA gene streptomycin resistant Nicotiana tabacum FEBS Lett. 219 343–346

    Google Scholar 

  • M Farooqui AV Rao T Jayasree A Sadanandam (1997) ArticleTitleInduction of atrazine resistance and somatic embryogenesis in Solanum melongena Theor. Appl. Genet. 95 702–705

    Google Scholar 

  • R Fluhr A Dvora E Galun M Edelman (1985) ArticleTitleEfficient induction and selection of chloroplast encoded antibiotic resistant mutants in Nicotiana Proc. Natl. Acad. Sci. 82 1485–1489

    Google Scholar 

  • H Fromm E Galun M Edelman (1989) ArticleTitleA novel site for streptomycin resistance in the 530 loop of chloroplast 16s ribosomal RNA Plant Mol. Biol. 12 499–505

    Google Scholar 

  • S Galili H Fromm D Auis M Edelman E Galun (1989) ArticleTitleRibosomal protein S12 and a site for streptomycin resistance in Nicotiana chloroplast Mol. Gen. Genet. 218 289–292

    Google Scholar 

  • CE Jansen EAM Snel MJE Akerboom HJJ Nijkamp J Hille (1990) ArticleTitleInduction of streptomycin resistance in the wild tomato Lycopersicon peruvianum Mol. Gen. Genet. 220 261–268

    Google Scholar 

  • P Maliga (1984) ArticleTitleIsolation and characterization of mutants in plant cell culture Ann. Rev. Plant Physiol. 35 519–542

    Google Scholar 

  • PF McCabe AM Timmons PJ Dix (1989) ArticleTitleA simple procedure for the isolation of streptomycin resistant plants in Solanaceae Mol. Gen. Genet. 216 132–137

    Google Scholar 

  • P Medgyesy (1990) Selection and analysis of cytoplasmic hybrids PJ Dix (Eds) Plant Cell Line Selection Velt Weinheim 287–316

    Google Scholar 

  • P Medgyesy L Menczel P Maliga (1980) ArticleTitleThe use of cytoplasmic streptomycin resistance chloroplast transfer Nicotiana tabacum into Nicotiana sylvestris and isolation of their somatic hybrids Mol. Gen. Genet. 179 693–698

    Google Scholar 

  • T Murashige F Skoog (1962) ArticleTitleRevised medium for rapid growth and bio-assays with tobacco tissue cultures Physiol. Plant. 15 473–497

    Google Scholar 

  • PK Pawar CS Pawar BA Narkhede NP Teli SR Bhalsing VL Maheshwari (2002) ArticleTitleA technique for rapid micropropagation of Solanum surattense Burm.f. Ind. J. Biotech. 1 201–204

    Google Scholar 

  • RN Prasad HC Chaturvedi (1978) ArticleTitleIn vitro induction of shoots and formation of plantlets from segments of leaf, stem and root of Solanum xanthocarpum schrad & Wendl Ind. J. Exp. Biol. 16 1121–1122

    Google Scholar 

  • RN Prasad AK Sharma HC Chaturvedi (1998) ArticleTitleAndrogenic stable somaclonal variant of Solanum surattense Burm.f Ind. J. Exp. Biol. 36 1007–1008

    Google Scholar 

  • AV Rao A Farooqui T Jayasree RV Ramana A Sadanandam (1993) ArticleTitleEMS-induced streptomycin resistance in Solanum melongena L Theor. Appl. Gent. 87 527–530

    Google Scholar 

  • AV Rao Ch Venu A Sadanandam (1997) ArticleTitleSelection of streptomycin and kanamycin resistance using nitrosomethylurea and Agrobacterium in Solanum sisymbrifolium Ind. J. Exp. Biol. 35 188–192

    Google Scholar 

  • A Sadanandam MA Farooqui (1991) ArticleTitleInduction and selection of lincomycin-resistant plants in Solanum melongena L Plant Sci. 79 237–239

    Google Scholar 

  • Sivarajan VV & Balachandran I (1999) Ayurvedic Drugs and Their Plant Sources. Oxford and IBH Publishing Co. Pvt. Ltd.

  • K Subhash P Venkataiah P Bhaskar (1996) ArticleTitleInduction of streptomycin-resistant plantlets in Capsicum annuum L. through mutagenesis in vitro Plant Cell Rep. 16 111–113

    Google Scholar 

  • Z Svab EC Harper JDG Jones P Maliga (1990) ArticleTitleAminoglycoside-3″-adenyltransferase, confers resistance to spectinomycin and streptomycin in Nicotiana tabacum Plant Mol. Biol. 14 197–205

    Google Scholar 

  • KY To CC Chem YK Lai (1989) ArticleTitleIsolation and char- acterization of streptomycin-resistant mutants in Nicotiana plumbaginifolia Theor. Appl. Genet. 78 81–86

    Google Scholar 

  • KY To YK Lai TY Feng CC Chem (1992) ArticleTitleRestriction endonuclease analysis of chloroplast DNA from streptomycin resistant mutants of Nicotiana plumbaginifolia Genome 35 220–224

    Google Scholar 

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Correspondence to N. Rama Swamy.

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Swamy, N.R., Ugandhar, T., Praveen, M. et al. Induction of streptomycin-resistant plantlets in Solanum surattense through in vitro mutagenesis. Plant Cell Tiss Organ Cult 80, 201–207 (2005). https://doi.org/10.1007/s11240-004-0737-x

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