Abstract
Two PCR-based techniques, RAPD and ISSR, were utilized for determination of genetic relationship of 24 strawberry cultivars used in breeding program at the Research Institute of Pomology and Floriculture in Poland. Polymorphism of investigated genotypes was observed in reactions with 23 out of 48 tested RAPD primes and 41 from 90 tested ISSR primers. Relationship, determined on the base of polymorphic products analysis and showed in the form of dendrograms (UPGMA percent method), was generally similar for both techniques, although similarity values based on ISSR data were higher than those based on RAPD. The parallel use of two data sets seems to allow for precise estimation of cultivars relationship and diminishing mistakes connected with methods' technical limitations.
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References
Arnau G, Lallemand J & Bourgoin M (2003) Fast and reliable strawberry cultivar identification using inter simple sequence repeat (ISSR) amplification. Euphytica 129: 69–79
Dale A (1996) A key and vegetative descriptions of thirty-two common strawberry varieties grown in North America. Adv. Strawberry Res. 15: 1–12
Degani C, Rowland LJ, Levi A, Hortynski JA & Galleta GJ (1998) DNA fingerprinting of strawberry (Fragaria ananassa) cultivars using randomly polymorphic DNA (RAPD) markers. Euphytica 102: 247–253
Degani C, Rowland LJ, Saunders JA, Hokanson SC, Ogden EL, Golan-Goldhirsh A & Galletta GJ (2001) A comparison of genetic relationship measures in strawberry (Fragaria × annanassa Duch.) based on AFLPs, RAPDs, and pedigree data. Euphytica 117: 1–12
Doyle JI & Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12: 13–15
Godwin ID, Aitken EA & Smith LW (1997) Application of inter simple sequence repeat (ISSR) markers to plant genetics. Electrophoresis 18: 1524–1528
Graham J, McNicol RJ, Greig K & Van de Ven WTG (1994) Identification of red raspberry cultivars and an assessment to their relatedness using fingerprinting produced by random primers. J. Hort. Sci. 69: 123–130
Graham J, McNicol RJ & McNicol JW (1996) A comparison of methods for the estimation of genetic diversity in strawberry cultivars. Theor. Appl. Genet. 93: 402–406
Joshi SP, Gupta VS, Aggarwal PK, Ranjekar PK & Brar DS (2000) Genetic diversity and phylogenetic relationship as revealed by inter-simple sequence repeat (ISSR) polymorphism in the genus Oryza. Theor. Appl. Genet. 100: 1311–1320
Landry BS, Li RQ, Cheung WY & Granger RL (1994) Phylogeny analysis of 25 apple rootstocks using RAPD markers and tactical tagging. Theor. Appl. Genet. 89: 847–852
Landry BS, Rongqi L & Khanizadeh S (1997) Classification of 75 strawberry cultivars and breeding lines using RAPD markers. Acta Hortic. 439: 101–105
Levi A & Rowland IJ (1997) Identifying blueberry cultivars and evaluating their genetic relationships using randomly amplified polymorphic DNA (RAPD) and simple sequence repeats (SSR) anchored primers. J. Am. Soc. Hort. Sci. 122: 74–78
Meunier JR & Grimont PAD (1993) Factors affecting reproducibility of random amplified polymorphic DNA fingerprinting. Res. Microbiol. 144: 373–379
Moreno S, Gogorcena Y & Ortiz JM (1995) The use of RAPD markers for identification of cultivated grapevine (Vitis vinifera L.). Sci. Hort. 62: 237–243
McGregor CE, Lambert CA, Greyling MM, Louw JH & Warnich L (2000) A comparative assessment of DNA fingerprinting techniques (RAPD, ISSR, AFLP and SSR) in tetraploid potato (Solanum tuberosum L.) germplasm. Euphytica 113: 135–144
Nybom H (1991) Application of DNA fingerprinting in plant breeding. Proc. Intern. Symp. DNA fingerprinting, Bern. 1991 (pp. 294–311)
Patzak J, Oriniaková P, Matoušek J & Svoboda P (1999) Czech hop characterization using RAPD method and genetic distance analysis of selected genotypes. Plant Prod. 45: 165–172
Patzak J (2001) Comparison of RAPD, STS, ISSR and AFLP molecular methods used for assessment of genetic diversity in hop (Humulus lupulus L.). Euphytica 121: 9–18
Powell W, Morgante M, Andre C, Hanafey M, Vogel J, Tingey S & Rafalski A (1996) The comparison of RAPD, RAPD, AFLP and SSR (microsatellite) markers for germoplasm nalysis. Mol. Breed. 2: 225–238
Reddy MP, Sarla N, Neeraja CN & Siddiq EA (2000) Assessing genetic variation among Asian A-genome Oryza species using inter simple sequence repeat (ISSR) polymorphism. Fourth International Rice Genetics Symposium, 22–27 October 2000 Abstracts (p. 212.) IRRI, Philippines
Skroch PW & Nienhuis J (1995) Impact of scoring error and reproducibility of RAPD data on RAPD-based estimates of genetic distance. Theor. Appl. Genet. 91: 1086–1091
Spangelo LPS, Hsu SS, Fejer SO & Watkins R (1971) Inbred line X tester analysis and the potential of inbreeding in strawberry breeding. Can. J. Genet. Cytol. 13: 460–469
Williams JGK, Kubelik AR, Livak KJ, Rafalski JA & Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl. Acids. Res. 18: 6531–6535
Zietkiewicz E, Rafalski A & Labuda D (1994) Genome fingerprinting by simple sequence repeat (SSR) – anchored polymerase chain reaction amplification. Genomics 20: 176–183
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Kuras, A., Korbin, M. & żurawicz, E. Comparison of suitability of RAPD and ISSR techniques for determination of strawberry (Fragaria × ananassa Duch.) relationship. Plant Cell, Tissue and Organ Culture 79, 189–193 (2004). https://doi.org/10.1007/s11240-004-0659-7
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DOI: https://doi.org/10.1007/s11240-004-0659-7