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Ultraviolet Rays Promote Development of Photosystem II Photochemical Activity and Accumulation of Phenolic Compounds in the Tea Callus Culture (Camellia sinensis)

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Abstract

Effect of UV-B rays (280–320 nm) on photosynthetic electron transport and production of phenolic compounds in tea (Camellia sinensis L.) callus culture grown in white light was investigated. When white light was supplemented with UV radiation, the culture growth was retarded and morphological characteristics were modified. These conditions promoted the formation of chlorophyll-bearing cells and altered the ability of cultured cells to accumulate phenolic compounds, including flavans specific to Camellia sinensis. By the end of the culturing cycle (on the 45th day), the total content of phenolic compounds in the culture grown under supplementary UV irradiation was almost 1.5 times higher than in the control culture. The UV rays greatly stimulated photosystem II (PSII) activity in phototrophic cells of the callus culture, which was indicated by a large increase in the ratio of variable chlorophyll fluorescence to maximal fluorescence. This ratio was as low as 0.19 in cells cultured in white light and increased to 0.53 in the cell culture grown under white and UV light. The kinetics of dark relaxation of chlorophyll variable fluorescence, related to reoxidation of PSII primary acceptor, contained either two or three components, depending on the absence or presence of UV radiation, respectively. An artificial electron acceptor of PSI, methyl viologen modified the kinetics of dark decay of chlorophyll variable fluorescence in a characteristic manner, implying that photosynthetic electron transport was mediated by PSI and PSII in both treatments (culturing in white light with and without UV-B). It is concluded that stimulatory effect of UV rays on the parameters examined in phototrophic regions of Camellia tissue culture is determined by photoexcitation of a regulatory pigment that absorbs quanta in blue and long-wave UV spectral regions.

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Abbreviations

PSI and PSII:

photosystems I and II

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Authors and Affiliations

  1. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya ul. 35, Moscow, 127276, Russia

    N. V. Zagoskina, A. K. Alyavina, T. O. Gladyshko, P. V. Lapshin, E. A. Egorova & N. G. Bukhov

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  1. N. V. Zagoskina
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  2. A. K. Alyavina
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  3. T. O. Gladyshko
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  4. P. V. Lapshin
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  5. E. A. Egorova
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__________

Translated from Fiziologiya Rastenii, Vol. 52, No. 6, 2005, pp. 830–838.

Original Russian Text Copyright © 2005 by Zagoskina, Alyavina, Gladyshko, Lapshin, Egorova, Bukhov.

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Zagoskina, N.V., Alyavina, A.K., Gladyshko, T.O. et al. Ultraviolet Rays Promote Development of Photosystem II Photochemical Activity and Accumulation of Phenolic Compounds in the Tea Callus Culture (Camellia sinensis). Russ J Plant Physiol 52, 731–739 (2005). https://doi.org/10.1007/s11183-005-0109-3

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